LOCUS       sequence-283095-        4844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2886
FEATURES             Location/Qualifiers
     source          1..4844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggccca gtcccacagc tccacgacca ctgaaaagaa aaaagttgag
       61 aattccatag tgaagtgctc cactcgaaca gatgtgagcg agaaagccgt tgcctccagc
      121 accacttcta atgaggatga aagtcctgga cagacttatc acagagagag aagaaacgca
      181 atcactatgc agccacagaa tgtccagggg ctcagcaaag tcagtgagga accttcaaca
      241 tcgagtgacg agagggcctc attgatcaag aaagagatcc atgggtccct gccacacgtg
      301 gcggagccct ctgtgccgta ccgcgggacg gtgtttgcca tggaccccag gaatggttac
      361 atggagcccc actaccaccc tcctcatctt ttccctgcct tccatcctcc tgtaccaatt
      421 gatgccagac atcatgaggg ccgttaccat tacgatccat ctccgattcc tccattgcat
      481 atgacttccg ccttatctag tagccctacg tatccggacc tgcccttcat taggatctcc
      541 ccacaccgga accccactgc tgcttccgag tctcccttca gccctccaca tccctacatt
      601 aatccctaca tggactatat ccgctccttg cacagcagcc catcgctctc catgatctca
      661 gcaacccgtg ggctgagccc tacagatgcg ccccatgcag gagtcagccc agcagaatac
      721 tatcatcaga tggccctgct aactggccag cgcagcccct atgcagacat tattccctca
      781 gctgccaccg ccggcacggg ggccatccac atggaatatc ttcatgctat ggatagcacc
      841 agattctcca gccccaggct gtcagccagg ccgagccgaa aacgtacact gtccatatca
      901 ccactctccg atcatagctt tgaccttcag accatgataa ggacgtctcc caactccttg
      961 gtcacgattc tcaataattc ccgtagcagc tcttcagcaa gtggctccta tggtcactta
     1021 tctgcaagtg caatcagccc tgccttgagc ttcacctact cttccgcgcc cgtctctctc
     1081 cacatgcatc agcagatcct aagccgacaa cagagcttag gttcagcctt tggacacagc
     1141 cctccactca tccaccctgc cccaactttt ccaacacaga ggcctattcc agggatccct
     1201 acggttctga accccgtcca ggtcagctcc ggcccttctg agtcctcaca gaacaagccc
     1261 acgagtgagt ctgcagtgag cagcactggt gacccgatgc acaacaagag gtccaagatc
     1321 aaacccgatg aagacctccc cagcccaggg gctcgggggc agcaggaaca gcccgaagga
     1381 acaacccttg tcaaggagga aggggacaaa gatgaaagca aacaggagcc tgaagtcatc
     1441 tatgagacaa actgccactg ggaaggctgc gcgagggagt tcgacaccca agagcagctt
     1501 gtgcaccata taaataacga ccatattcat ggagagaaga aggagttcgt gtgcaggtgg
     1561 ctggactgct caagagagca gaaacccttc aaagcccagt atatgttggt agtgcatatg
     1621 agaagacaca cgggcgagaa gcctcacaaa tgcacttttg aaggttgcac aaaggcctac
     1681 tcgagactag aaaacttgaa aacacacttg agatctcaca ctggagagaa accatacgtc
     1741 tgtgagcacg aaggttgcaa caaggctttc tcaaatgcct ctgatcgcgc caaacaccaa
     1801 aacagaacgc attccaatga gaaaccatat gtgtgcaaaa tcccaggctg cactaagcgt
     1861 tacacagacc caagctccct ccggaaacat gtgaagacag tgcatggccc agaggctcat
     1921 gtcaccaaga agcagcgagg ggacatccat cctcggccgc cacccccgag agattccggc
     1981 agccattcac agtccaggtc gcctggccga ccgactcagg gagcccttgg tgagcagcag
     2041 gacctcagca acactacctc aaagcgggaa gaatgcctcc aggtgaaaac cgtcaaggca
     2101 gagaagccaa tgacatctca gccaagccct ggtggtcagt cttcatgcag cagccaacag
     2161 tcccccatca gcaactattc caacagtggg ctcgagcttc ctctgaccga tggaggtagt
     2221 ataggagacc tcagtgccat cgatgaaacc ccaatcatgg actcaaccat ttccactgca
     2281 accacagccc ttgctttgca agccaggaga aacccggcag ggaccaaatg gatggagcac
     2341 gtaaaactag aaaggctaaa acaagtgaat ggaatgtttc cgcgactgaa ccccattcta
     2401 ccccctaaag cccctgcggt ctctcctctc ataggaaatg gcacacagtc caacaacacc
     2461 tgcagcttgg gtgggcccat gacgcttctc ccgggcagaa gcgacctctc tggggtggac
     2521 gtcactatgc tgaacatgct caacagaagg gacagcagcg ccagcaccat cagctcggcc
     2581 tacctgagca gccgccgctc ctcagggatc tcgccctgct tctccagccg ccgctccagc
     2641 gaggcgtcac aggccgaggg ccggccgcag aacgtgagcg tggccgactc ctacgacccc
     2701 atctccaccg acgcctcgcg ccgctccagc gaagccagcc agagcgacgg cctgcccagc
     2761 ctgctcagcc tcacgcccgc ccagcagtac cgcctcaagg ccaagtacgc ggctgccaca
     2821 ggagggccgc cgccgacgcc cctgcccaac atggagagga tgagcctgaa gacgcgcctg
     2881 gcgctgctcg gggatgccct cgagcctggc gtggccctgc ctccagttca tgccccgagg
     2941 aggtgcagcg acgggggagc ccacggctac gggcggcgcc acctgcagcc gcacgatgcg
     3001 ccgggccacg gcgtgaggag ggccagcgac ccggtgcgga caggctccga gggcctggcc
     3061 ctgcctcgtg tgccgcgctt cagcagcctc agcagctgca accccccggc gatggccacg
     3121 tccgcggaga agcgcagtct cgtgcttcag aattacacgc ggcccgaggg cggccagtcc
     3181 cgaaacttcc actcgtcccc ctgtcctccc agcatcaccg agaacgtcac cctggagtcc
     3241 ctgaccatgg acgctgatgc caacctgaac gatgaggatt tcctgccgga cgacgtggtg
     3301 cagtatttaa attcccagaa ccaagcaggg tacgagcagc acttccccag cgccctcccg
     3361 gacgacagca aagtgcccca cgggcccggt gactttgacg cgcccgggct gccagacagc
     3421 cacgctggcc agcagttcca tgccctcgag cagccctgcc ccgagggcag caaaaccgac
     3481 ctgcccattc agtggaacga agtcagctcc ggaagcgccg acctgtcctc ctccaagctc
     3541 aagtgtgggc cgcggcccgc tgtgccgcag actcgcgcct ttgggttctg caacggcatg
     3601 gtcgtccacc cgcagaaccc cttgaggagc gggcctgctg ggggctatca gaccctcggg
     3661 gagaacagca acccctacgg tggcccagag cacttgatgc tccacaacag ccccggaagt
     3721 ggcaccagtg gaaacgcctt ccatgaacag ccctgtaagg ccccgcagta tgggaactgt
     3781 ctcaacaggc agccagtggc ccctggtgca ctcgacggtg cctgtggtgc cgggattcaa
     3841 gcctcaaagc tgaagagcac ccccatgcaa gggagcgggg gccagctgaa tttcggcctg
     3901 ccggtagcgc caaatgagtc agctggcagc atggtgaatg gcatgcagaa ccaggaccca
     3961 gtgggacagg ggtacctggc tcaccagctc ctcggcgaca gcatgcagca cccgggggca
     4021 ggccgccccg gtcagcagat gcttgggcag attagtgcta cctcacacat caacatctac
     4081 caagggccag agagctgcct gccaggggct cacggcatgg gcagccagcc gtcaagcttg
     4141 gcagttgtca ggggctacca gccatgtgcc agctttgggg gcagcaggcg ccaggctatg
     4201 ccgagggaca gccttgctct gcagtcagga cagctcagtg acacaagtca gacctgcagg
     4261 gtgaatggta tcaagatgga gatgaaaggg cagccccatc cgctgtgctc taatctgcag
     4321 aattactctg gtcagttcta tgaccaaacc gtgggcttca gtcagcaaga cacgaaagct
     4381 ggttcattct ctatttcaga cgccagctgc ctgctacagg ggaccagcgc caaaaactct
     4441 gagttacttt ccccaggtgc taatcaggtg acaagcacag tggacagcct cgacagccat
     4501 gacctggaag gggtacagat tgacttcgat gccatcatag acgatgggga ccactccagc
     4561 ctgatgtcgg gggccctgag cccaagtatc attcagaacc tttcccatag ctcctcccgc
     4621 ctcaccacgc ctcgggcgtc cctcccattc ccagcgctgt ccatgagcac caccaacatg
     4681 gctatcgggg acatgagttc tttgctgacc tccctagcgg aagaaagcaa attccttgca
     4741 gttatgcaat agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     4801 atatcttgtg gaaaggacga cacaattgcc ataatcggaa ctgt
//
