LOCUS       sequence-283101-        1886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2900
FEATURES             Location/Qualifiers
     source          1..1886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggccaa catgtaccgg gtcggagatt atgtctactt tgagaattcc
       61 tccagcaacc catacctaat aagaaggata gaagaactca acaagactgc aagtggcaac
      121 gtggaagcaa aagtagtatg cttttataga cgacgtgata tttccaacac acttataatg
      181 ctcgcagata agcatgctaa agaaattgag gaagaatctg aaacaacagt tgaggctgac
      241 ttgaccgata agcagaaaca tcagttgaaa catagggaac tctttttgtc acgccagtat
      301 gaatctctgc ccgcaacaca tatcagggga aagtgcagtg ttgcccttct gaatgagaca
      361 gaatcagtat tgtcatatct tgataaggag gataccttct tctactcatt ggtctatgac
      421 ccctcattga aaacactatt agctgacaaa ggtgaaatca gagtgggacc tagatatcaa
      481 gcagacattc cagaaatgct gttagaagga gaatcagatg agagggaaca atcaaaattg
      541 gaagttaaag tttgggatcc aaatagccca cttacggatc gacagattga ccagttttta
      601 gttgtagcac gtgctgttgg gacattcgcc agagccctgg attgcagcag ttctgtgagg
      661 cagcctagtt tgcatatgag tgctgctgca gcttcccgag acatcacctt gtttcacgct
      721 atggatacat tgtatagaca cagctatgat ttgagcagtg ccattagtgt cttagtacca
      781 ctcggaggac ctgttttatg cagagatgaa atggaggaat ggtcagcctc tgaagctagc
      841 ttatttgaag aggcactgga aaaatatggc aaagacttca atgacatacg gcaagatttt
      901 cttccttgga aatcattgac tagcatcatt gaatattatt acatgtggaa aactactgac
      961 agatatgtgc aacagaaacg tctaaaagca gcagaagctg agagtaaact gaaacaagta
     1021 tatatcccaa cctacagcaa accaaatccc aaccaaatat ccactagtaa tgggaagcct
     1081 ggtgctgtga atggagctgt ggggaccacg ttccagcctc agaatcctct cttagggaga
     1141 gcctgtgaga gctgctatgc tacacagtct caccagtggt attcttgggg cccacctaat
     1201 atgcagtgta gattatgtgc aatttgttgg ctttattgga aaaaatatgg aggcttgaaa
     1261 atgcccaccc agtcagaaga agagaagtta tctcctagcc caactacaga ggaccctcgt
     1321 gttagaagtc acgtgtcccg ccaggccatg cagggaatgc cagtccgaaa cactgggagt
     1381 ccaaagtctg cagtgaagac ccgccaagct ttcttccttc atactacata tttcacaaaa
     1441 tttgctcgtc aggtctgcaa aaataccctc cggctgcggc aggcagcaag acggccgttt
     1501 gttgctatta attatgctgc cattagggca gaatatgcag acagacatgc tgaactatct
     1561 ggaagtccac tgaaaagcaa aagcactagg aagcctttgg catgtatcat tgggtattta
     1621 gagatccatc ctgcaaagaa acctaatgta attcgatcta caccaagcct gcaaacccca
     1681 actaccaagc ggatgctaac aactccaaat cacacatctc tgagcattct ggggaaaaga
     1741 aactacagtc atcacaatgg tctggatgaa ctcacgtgct gtgtgtcaga ctagtaatga
     1801 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1861 gatcaaaggc tcaacacctg ataggt
//
