LOCUS       sequence-283107-        2378 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2903
FEATURES             Location/Qualifiers
     source          1..2378
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagtgcc ggcggctttg atccctccga cccagctggt cccccctcaa
       61 cccccaatct ccacgtctgc ttcctcctct ggcaccacca cctccacctc ttcggcgact
      121 tcgtctccgg ctccttccat cggacccccg gcgtcctctg ggccaactct gttccgcccg
      181 gagcccatcg cttcggcggc ggcggcggcg gccacagtca cctctaccgg cggcggcggc
      241 ggcggcggcg gcagcggagg cggcggcggc agcagcggca acggaggcgg cggtggcggc
      301 ggcggcggtg gcagcaactg caaccccaac ctggcggccg cgagcaacgg cagcggcggc
      361 ggcggcggcg gcatcagcgc tggcggcggc gtcgcttcca gcacccccat caacgccagc
      421 accggcagca gcagcagcag cagtagcagc agcagcagca gcagcagtag tagcagcagc
      481 agcagtagca gcagcagctg cggccccctc cccgggaaac ccgtgtactc aaccccgtcc
      541 ccagtggaaa acacccctca gaataatgag tgcaaaatgg tggatctgag gggggccaaa
      601 gtggcttcct tcacggtgga gggctgcgag ctgatctgcc tgccccaggc tttcgacctg
      661 ttcctgaagc acttggtggg gggcttgcat acggtctaca ccaagctgaa gcggctggag
      721 atcacgccgg tggtgtgcaa tgtggaacaa gttcgcatcc tgaggggact gggcgccatc
      781 cagccaggag tgaaccgctg caaactcatc tccaggaagg acttcgagac cctctacaat
      841 gactgcacca acgcaagttc tagacctgga aggcctccta agaggactca aagtgtcacc
      901 tccccagaga actctcacat catgccgcat tctgtccctg gtctcatgtc tcctgggata
      961 attccaccaa caggtctgac agcagccgct gcagcagctg ctgctgctac caatgcagct
     1021 attgctgaag caatgaaggt gaaaaaaatc aaattagaag ccatgagcaa ctatcatgcc
     1081 agtaataacc aacatggagc agactctgaa aacggggaca tgaattcaag tgtcggcagc
     1141 agtgatggtt cctgggataa ggaaacactg ccctcttccc catcccaggg acctcaggcc
     1201 tctataaccc acccccgcat gcctggagca cgtagccttc cacttagtca tcctctcaac
     1261 catcttcagc agagccacct tctgccaaat ggactggaac ttccttttat gatgatgccc
     1321 caccctctaa ttcctgtcag cctacctcca gcatctgtca ccatggcaat gagccagatg
     1381 aaccacctca gcaccattgc aaatatggca gcagcagcac aagttcagag tcccccatcc
     1441 agagttgaga catcagttat taaggagcgt gttcctgata gcccctcacc tgccccctct
     1501 ctggaggagg ggagaaggcc tggcagtcac ccatcatcac atcgcagcag cagcgtgtcc
     1561 agctcccctg ctcggactga gagctcttct gacagaatcc cggtccatca gaatgggttg
     1621 tccatgaacc agatgctgat gggcttatca ccaaatgtac ttcctgggcc caaagaggga
     1681 gatttggccg gtcatgacat gggacatgag tcaaaaagga tgcatattga aaaagatgag
     1741 accccgcttt ctacaccaac cgcaagagac agccttgaca aactctctct aactgggcat
     1801 ggacaaccac tgcctccagg ttttccatct ccttttctgt ttcctgatgg actgtcttcc
     1861 atcgagactc ttctgactaa catacagggg ctgttgaaag ttgccataga taatgccaga
     1921 gctcaagaga aacaggtcca actggaaaaa actgagctga agatggattt tttaagggaa
     1981 agagaactaa gggaaacact tgagaagcag ttggctatgg aacaaaagaa tagagccata
     2041 gttcaaaaga ggctaaagaa ggagaagaag gcaaagagaa aattgcagga agcacttgag
     2101 tttgagacga aacggcgtga acaagcagaa cagacgctaa aacaggcagc ttcaacagat
     2161 agtctcaggg tcttaaatga ctctctgacc ccagagatag aggctgaccg cagtggcggc
     2221 agaacagatg ctgaaaggac aatacaagat ggaagactgt atttgaaaac tactgtcatg
     2281 tactagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     2341 tgtggaaagg acgatataga cagtcccgcc tttcaatg
//
