LOCUS       sequence-283110-        1901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2905
FEATURES             Location/Qualifiers
     source          1..1901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgtctc cgcatctcca gtgatctctg caacttccag cggcgccggc
       61 gtcccggggg gcttattccg ggccgaaccc ctgtactcga ctcccagaga gccccctcgt
      121 cttactccta atatgatcaa tagtttcgtg gttaataacc acagcaacag tgccggaggc
      181 ggcggcaggg gcaacaccaa caccaacgag tgccgcatgg tcgacatgca cgggatgaag
      241 gtggcttcgt tcctgatgga cggccaggaa ctgatctgcc tgccgcaagt ctttgatctt
      301 tttctcaagc acctggtggg aggcttgcac actgtgtaca ccaagctgaa gagactggat
      361 atatcccccg tggtgtgtac tgttgagcag gtccggatcc tccgcgggct gggggccatc
      421 cagcccgggg taaaccgctg caaactcatc accaggaaag acttcgaaac tttgttcacc
      481 gattgcacca atgccagaag gaagaggcaa atgacaagaa aacaagctgt taacagttca
      541 agacccggca ggccccctaa gcgttctttg ggagtgttgc aggaaaatgc ccgccttctg
      601 acccatgcag tcccaggcct cttatcgcca ggacttatca ctccgacagg tataacagct
      661 gcagcgatgg ctgaggcgat gaaacttcag aagatgaagc ttatggctat gaacactctt
      721 cagggaaatg gaagccaaaa tgggaccgaa tcagagcctg atgatcttaa ttctaacaca
      781 ggtggaagtg aatcctcctg ggataaagat aagatgcagt ctccatttgc tgcacctgga
      841 ccccaacatg gaattgctca tgcagcccta gctggccagc caggcattgg gggtgctcca
      901 accctcaatc cactgcagca gaaccacctg ctaaccaata gactggatct gccatttatg
      961 atgatgcctc atcccctact tccagtcagc ttacctcctg catcagttgc catggcaatg
     1021 aatcagatga accatctcaa tactattgcc aacatggctg ctgcagcaca gattcacagt
     1081 ccactctcca gagctggtac ctctgttata aaggagcgga tcccagagag tccttctcct
     1141 gctccttctc tagaagagaa tcatcgtcct gggagccaga cctcttccca caccagcagc
     1201 agtgtgtcca gctctccctc tcagatggat catcatttgg aaagaatgga agaggtacca
     1261 gttcaaattc caataatgaa gtcacccttg gacaagatac agctgactcc tgggcaggca
     1321 ttgcccgctg gattccctgg accattcatt tttgctgata gtctgtcctc cgtggagact
     1381 ctgttgacca acattcaggg tctgctgaaa gttgctttgg ataatgctcg catccaggag
     1441 aagcagattc aacaagaaaa gaaggagctg cgactggagc tctatagaga gagagaaatt
     1501 agagaaaacc ttgaaagaca acttgcagtt gagcttcaaa gcagaactac tatgcaaaag
     1561 cgcctgaaga aggagaaaaa aaccaagaga aaattgcagg aagccttgga atttgaatca
     1621 aagcgccggg agcaagtgga gcaggcactt aagcaagcca ccactagtga cagtggcctg
     1681 aggatgttaa aagatactgg aattccagat attgaaatag aaaacaatgg gactcctcat
     1741 gatagtgctg ctatgcaagg aggtaactat tactgtttag aaatggcaca acagttgtat
     1801 tcagcctagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1861 tcttgtggaa aggacgacgt tactaccgat gttctattgg c
//
