LOCUS       sequence-283116-        1817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2907
FEATURES             Location/Qualifiers
     source          1..1817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgtctc cgcatctcca gtgatctctg caacttccag cggcgccggc
       61 gtcccggggg gcttattccg ggccgaaccc ctgtactcga ctcccagaga gccccctcgt
      121 cttactccta atatgatcaa tagtttcgtg gttaataacc acagcaacag tgccggaggc
      181 ggcggcaggg gcaacaccaa caccaacgag tgccgcatgg tcgacatgca cgggatgaag
      241 gtggcttcgt tcctgatgga cggccaggaa ctgatctgcc tgccgcaagt ctttgatctt
      301 tttctcaagc acctggtggg aggcttgcac actgtgtaca ccaagctgaa gagactggat
      361 atatcccccg tggtgtgtac tgttgagcag gtccggatcc tccgcgggct gggggccatc
      421 cagcccgggg taaaccgctg caaactcatc accaggaaag acttcgaaac tttgttcacc
      481 gattgcacca atgccagttc aagacccggc aggcccccta agcgttcttt gggagtgttg
      541 caggaaaatg cccgccttct gacccatgca gtcccaggcc tcttatcgcc aggacttatc
      601 actccgacag gtataacagc tgcagcgatg gctgaggcga tgaaacttca gaagatgaag
      661 cttatggcta tgaacactct tcagggaaat ggaagccaaa atgggaccga atcagagcct
      721 gatgatctta attctaacac aggtggaagt gaatcctcct gggataaaga taagatgcag
      781 tctccatttg ctgcacctgg accccaacat ggaattgctc atgcagccct agctggccag
      841 ccaggcattg ggggtgctcc aaccctcaat ccactgcagc agaaccacct gctaaccaat
      901 agactggatc tgccatttat gatgatgcct catcccctac ttccagtcag cttacctcct
      961 gcatcagttg ccatggcaat gaatcagatg aaccatctca atactattgc caacatggct
     1021 gctgcagcac agattcacag tccactctcc agagctggta cctctgttat aaaggagcgg
     1081 atcccagaga gtccttctcc tgctccttct ctagaagaga atcatcgtcc tgggagccag
     1141 acctcttccc acaccagcag cagtgtgtcc agctctccct ctcagatgga tcatcatttg
     1201 gaaagaatgg aagaggtacc agttcaaatt ccaataatga agtcaccctt ggacaagata
     1261 cagctgactc ctgggcaggc attgcccgct ggattccctg gaccattcat ttttgctgat
     1321 agtctgtcct ccgtggagac tctgttgacc aacattcagg gtctgctgaa agttgctttg
     1381 gataatgctc gcatccagga gaagcagatt caacaagaaa agaaggagct gcgactggag
     1441 ctctatagag agagagaaat tagagaaaac cttgaaagac aacttgcagt tgagcttcaa
     1501 agcagaacta ctatgcaaaa gcgcctgaag aaggagaaaa aaaccaagag aaaattgcag
     1561 gaagccttgg aatttgaatc aaagcgccgg gagcaagtgg agcaggcact taagcaagcc
     1621 accactagtg acagtggcct gaggatgtta aaagatactg gaattccaga tattgaaata
     1681 gaaaacaatg ggactcctca tgatagtgct gctatgcaag cctagtaatg aactagtccg
     1741 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgattccgtg
     1801 gatcgcacgg gcaatta
//
