LOCUS       sequence-283149-        1607 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2922
FEATURES             Location/Qualifiers
     source          1..1607
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatgaatt tcatcctttc atcgaagcac ttctgcccca cgtccgagcc
       61 tttgcctaca catggttcaa cctgcaggcc cgaaaacgaa aatacttcaa aaaacatgaa
      121 aagcgtatgt caaaagaaga agagagagcc gtgaaggatg aattgctaag tgaaaaacca
      181 gaggtcaagc agaagtgggc atctcgactt ctggcaaagt tgcggaaaga tatccgaccc
      241 gaatatcgag aggattttgt tcttacagtt acagggaaaa aacctccatg ttgtgttctt
      301 tccaacccag accagaaagg caagatgcga agaattgact gcctccgcca ggcagataaa
      361 gtctggaggt tggaccttgt tatggtgatt ttgtttaaag gtattccgct ggaaagtact
      421 gatggcgagc gccttgtaaa gtccccacaa tgctctaatc cagggctctg tgtccaaccc
      481 catcacatag gggtttctgt taaggaactc gatttatatt tggcatactt tgtgcatgca
      541 gcagattcaa gtcaatctga aagtcccagc cagccaagtg acgctgacat taaggaccag
      601 ccagaaaatg gacatttggg cttccaggac agttttgtca catcaggtgt ttttagtgtc
      661 actgagctag taagagtgtc acagacacca atagctgcag gaactggccc aaatttttct
      721 ctctcagatt tggaaagttc ttcatactac agcatgagtc caggagcaat gaggaggtct
      781 ttacccagca catcctctac gagctccaca aagcgcctca agtctgtgga ggatgaaatg
      841 gacagtcctg gtgaggagcc attttataca ggccaagggc gctccccagg aagtggcagt
      901 cagtcaagtg gatggcatga agtggagcca ggaatgccat ctccaaccac actgaagaag
      961 tcggagaagt ctggtttcag cagcccctcc ccttcacaga cctcctccct gggaacggcg
     1021 ttcacacagc atcaccgacc tgtcattaca ggacccagag caagtccgca tgcaacacca
     1081 tcgactcttc atttcccgac atcacccatt atccagcagc ctgggcctta cttctcacac
     1141 ccagccatcc gctatcaccc tcaggagacg ctgaaagaat ttgtccaact tgtctgccct
     1201 gatgctggtc agcaggctgg acaggtgggg ttcctcaatc ccaatgggag cagccaaggc
     1261 aaggtgcaca acccattcct tcccacccca atgttgccac cgccaccgcc accaccgatg
     1321 gccaggcctg tgcctctgcc ggtgccagac acaaagcctc caaccacgtc aacagaagga
     1381 ggtgcagcct cccccacgtc accaacctac tcgacaccca gcacctcccc cgcaaaccga
     1441 ttcgtcagtg ttggaccacg ggatccaagc tttgtaaata tccctcaaca gacacagtcc
     1501 tggtacctgg gatagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1561 tatatatctt gtggaaagga cgactaaagc gggacgctaa gttacgg
//
