LOCUS       sequence-28315-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Nam HS, Benezra R
  TITLE     High levels of Id1 expression define B1 type adult neural stem 
            cells.
  JOURNAL   Cell Stem Cell. 2009 Nov 6. 5(5):515-26.
  PUBMED    19896442
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2009 Nov 6. 5(5):515-26."
COMMENT     Sequence Label: pIRES-Venus
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(37..288)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     428..447
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     687..708
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctttaggtga cctatagaat agggccctct agattacttg tacagctcgt ccatgccgag
       61 agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg
      121 gtctttgctc agggcggact ggtagctcag gtagtggttg tcgggcagca gcacggggcc
      181 gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgccgc cgtcctcgat
      241 gttgtggcgg atcttgaagt tggccttgat gccgttcttc tgcttgtcgg cggtgatata
      301 gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt
      361 gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc
      421 ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc
      481 ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcgggcgaa
      541 gcactgcagg ccgtagccca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc
      601 ggtggtgcag atcagcttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc
      661 ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac
      721 ccccggtgaa cagctcctcg cccttgctca ccatgg
//
