LOCUS       sequence-283176-        2093 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0027
FEATURES             Location/Qualifiers
     source          1..2093
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccctgtg ttatggaact ttctggggct accctaagat gctggaagct
       61 gccaatctca tggagggcct agtggatatc ggcccttggg tcactcttcc cagaggacag
      121 cctgaggtgt tggagtgggg cctcccaaag gatcaggact cagtggcctt tgaggatgtg
      181 gctgtgaact tcacccatga ggagtgggct ttgctgggtc catcacagaa gaatctctac
      241 agagatgtga tgcgagaaac cattaggaac ctgaactgta taggaatgaa atgggaaaac
      301 cagaacattg atgatcagca ccaaaatctc aggagaaatc caaggtgtga tgtggtagag
      361 agatttggta aaagtaaaga tggtagtcag tgtggagaaa ccttaagcca gattcgaaat
      421 agtattgtaa acaagaacac tcccgccaga gtagatgcat gtggaagcag tgtgaatgga
      481 gaagtcataa tgggtcattc atccctgaat tgctacatca gagttgatac tggacacaaa
      541 caccgggagt gtcatgaata tgcagagaag tcatatacac ataagcagtg tgggaaaggc
      601 ttaagttatc gccactcctt tcaaacatgt gaaaggcctc acactggaaa gaaaccctat
      661 gattgtaagg aatgtggaaa aaccttcagt tctcctggaa accttcgaag acatatggta
      721 gtaaaaggtg gagatggacc ttataaatgt gaattgtgtg ggaaagcctt tttttggccc
      781 agtttattac gtatgcatga aagaactcac actggagaga aaccatatga atgtaagcag
      841 tgttctaaag ccttccctgt ttacagttcc tatctaagac atgaaaaaat acacactggg
      901 gagaaaccgt atgaatgtaa gcagtgttct aaagccttcc ctgattacag ttcatatcta
      961 agacatgaaa gaactcacac tggagagaaa ccctacaaat gtaaacaatg tgggaaagcc
     1021 ttcagtgttt ccggttccct tcgagtacat gaaagaattc acactggaga gaaaccctat
     1081 acatgtaaac agtgtgggaa agcgttttgt catcttggaa gctttcaaag acacatgata
     1141 atgcacagtg gagatggacc tcataaatgt aagatatgtg ggaaaggctt tgattttcct
     1201 ggttcagcac gaattcatga aggaactcac actctagaga aaccctatga atgtaagcaa
     1261 tgtgggaaat tgttatctca tcgctcaagc tttcgaagac acatgatggc acacactgga
     1321 gatggccctc ataaatgcac agtatgtggg aaagcctttg attctcctag tgtatttcaa
     1381 agacatgaaa ggactcacac tggagagaaa ccctatgaat gcaagcaatg tgggaaagcc
     1441 ttccgtactt ccagttccct tcgaaaacat gaaacaacac acactggaga gcaaccctat
     1501 aaatgtaaat gtggaaaagc ttttagtgat ttattttcct ttcaaagtca tgaaacaaca
     1561 cacagtgaag aggagcctta tgaatgtaag gagtgtggga aagcatttag ttcttttaaa
     1621 tacttttgtc gccatgaaag gactcacagt gaagaaaaat cttatgagtg tcaaatttgt
     1681 ggcaaagcct tcagtcgttt cagttactta aaaactcatg aaaggactca cacggcagag
     1741 aagccatatg aatgtaagca atgcaggaaa gcattctttt ggccctcttt ccttctaaga
     1801 catgaaagga ctcacactgg agaaagaccc tatgaatgta aacactgtgg taaagccttc
     1861 agtcgttcca gtttctgtcg agaacatgaa agaactcaca ctggagagaa gccctatgaa
     1921 tgtaaggaat gtgggaaagc cttcagttct ctcagttcct ttaatagaca taaaaggaca
     1981 cactggaagg atattctata gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     2041 tggctttata tatcttgtgg aaaggacgac tgcagattga gcgggacaaa caa
//
