LOCUS       sequence-283179-        1949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0028
FEATURES             Location/Qualifiers
     source          1..1949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggactcagt ggcctttgag gatgtggctg tgaacttcac ccatgaggag
       61 tgggctttgc tgggtccatc acagaagaat ctctacagag atgtgatgcg agaaaccatt
      121 aggaacctga actgtatagg aatgaaatgg gaaaaccaga acattgatga tcagcaccaa
      181 aatctcagga gaaatccaag gtgtgatgtg gtagagagat ttggtaaaag taaagatggt
      241 agtcagtgtg gagaaacctt aagccagatt cgaaatagta ttgtaaacaa gaacactccc
      301 gccagagtag atgcatgtgg aagcagtgtg aatggagaag tcataatggg tcattcatcc
      361 ctgaattgct acatcagagt tgatactgga cacaaacacc gggagtgtca tgaatatgca
      421 gagaagtcat atacacataa gcagtgtggg aaaggcttaa gttatcgcca ctcctttcaa
      481 acatgtgaaa ggcctcacac tggaaagaaa ccctatgatt gtaaggaatg tggaaaaacc
      541 ttcagttctc ctggaaacct tcgaagacat atggtagtaa aaggtggaga tggaccttat
      601 aaatgtgaat tgtgtgggaa agcctttttt tggcccagtt tattacgtat gcatgaaaga
      661 actcacactg gagagaaacc atatgaatgt aagcagtgtt ctaaagcctt ccctgtttac
      721 agttcctatc taagacatga aaaaatacac actggggaga aaccgtatga atgtaagcag
      781 tgttctaaag ccttccctga ttacagttca tatctaagac atgaaagaac tcacactgga
      841 gagaaaccct acaaatgtaa acaatgtggg aaagccttca gtgtttccgg ttcccttcga
      901 gtacatgaaa gaattcacac tggagagaaa ccctatacat gtaaacagtg tgggaaagcg
      961 ttttgtcatc ttggaagctt tcaaagacac atgataatgc acagtggaga tggacctcat
     1021 aaatgtaaga tatgtgggaa aggctttgat tttcctggtt cagcacgaat tcatgaagga
     1081 actcacactc tagagaaacc ctatgaatgt aagcaatgtg ggaaattgtt atctcatcgc
     1141 tcaagctttc gaagacacat gatggcacac actggagatg gccctcataa atgcacagta
     1201 tgtgggaaag cctttgattc tcctagtgta tttcaaagac atgaaaggac tcacactgga
     1261 gagaaaccct atgaatgcaa gcaatgtggg aaagccttcc gtacttccag ttcccttcga
     1321 aaacatgaaa caacacacac tggagagcaa ccctataaat gtaaatgtgg aaaagctttt
     1381 agtgatttat tttcctttca aagtcatgaa acaacacaca gtgaagagga gccttatgaa
     1441 tgtaaggagt gtgggaaagc atttagttct tttaaatact tttgtcgcca tgaaaggact
     1501 cacagtgaag aaaaatctta tgagtgtcaa atttgtggca aagccttcag tcgtttcagt
     1561 tacttaaaaa ctcatgaaag gactcacacg gcagagaagc catatgaatg taagcaatgc
     1621 aggaaagcat tcttttggcc ctctttcctt ctaagacatg aaaggactca cactggagaa
     1681 agaccctatg aatgtaaaca ctgtggtaaa gccttcagtc gttccagttt ctgtcgagaa
     1741 catgaaagaa ctcacactgg agagaagccc tatgaatgta aggaatgtgg gaaagccttc
     1801 agttctctca gttcctttaa tagacataaa aggacacact ggaaggatat tctatagtaa
     1861 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1921 gacgagccta cgggactttc ccatcctac
//
