LOCUS       sequence-283185-        1808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0041
FEATURES             Location/Qualifiers
     source          1..1808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggactgtt gacatttagg gatgtggcca tagaattctc tctggaagaa
       61 tggcaatgcc tggacactgc acagcggaat ttatataaaa atgtgatttt agagaactac
      121 agaaacctgg tcttcctggg tattgctgtc tctaagcaag acctgatcac ctgtctggag
      181 caagaaaaag agcctttgac tgtgaagaga catgagatgg tgaatgaacc cccagtaatg
      241 tgttctcatt ttgcccaaga gttttggcca gagcagaaca taaaagattc ttttgaaaaa
      301 gtgacactga gaagatatga aaaatgtgga aatgataatt ttcagttaaa aggctgtaaa
      361 agtgtggatg aatgtaagtt gcacaaggga ggttataatg gacttaacca atgtttacca
      421 actatgcaga gcaaaatgtt tcaatgtgat aaatatgtga aagtctttaa taaattttca
      481 cattcagata gacataagat aaaacatatg gaaaataaac ctttcaaatg taaagaatgt
      541 ggcagatcat tttgcatgct ttcacaccta actcgacatg aaagaaatta taccaaggtg
      601 aatttctgca aatgtgaaga atgtgaaaaa gctgttaacc aatcttcaaa gcttactaaa
      661 cataaaagaa tttatacttg tgagaaactc tacaaatgtc aagaatgtga cagaactttt
      721 aaccaattct caaaccttac tgaatataaa aaagattatg ctcgagagaa accatacaaa
      781 tgtgaagaat gtggcaaagc ctttaaccag tcctcacacc ttactacaca taagataatt
      841 catacaggag agaaacccta caaatgtgaa gaatgtggca aagcctttaa ccagttctca
      901 aatcttacta cacataaaaa aattcatact ggagagcaac cctacatatg tgaagaatgt
      961 ggcaaggctt ttacccaatc ctcaaccctt actacacata agagaattca tactggagaa
     1021 aaaccctaca aatgtgaaga atgtggaaaa gcttttaacc gatcctcaaa acttactgaa
     1081 cataaaaaca ttcatactgg agagcaaccc tacaaatgtg aggaatgcgg caaagctttt
     1141 aaccgatcct caaatcttac ggaacatagg aaaattcata ccgaagagaa accctacaaa
     1201 tgtaaagaat gtggcaaagc ttttaaacac tcctcagccc ttactacaca taagagaatt
     1261 cacactggag agaaacccta caaatgtgaa gaatgtggca aagcttttaa ccgatcctca
     1321 aaacttactg aacataagaa acttcatact ggaaagaaac cctacaaatg tgaagaatgt
     1381 ggcaaagctt ttatccaatc ctcaaaactt actgaacata agaaaattca ttctggagag
     1441 ataccctaca agtgtgaaga atgtggcaaa gcttttaaac actcctcatc ccttactaca
     1501 cataaaagaa ttcatactgg ggagaaaccc tacaaatgtg aagaatgtgg caaagctttt
     1561 agccgatcct caaaacttac tgaacataag ataattcata ctggagagaa accctataaa
     1621 tgtgagagat gtgacaaagc ttttaaccaa tctgcaaacc ttactaaaca taaaaaaata
     1681 catactggag agaaactaca gaactggaat gtgtagtaat gaactagtcc gtaacttgaa
     1741 agtatttcga tttcttggct ttatatatct tgtggaaagg acgatcacac ttgataggaa
     1801 gccaacta
//
