LOCUS       sequence-283188-        1952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0229
FEATURES             Location/Qualifiers
     source          1..1952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaactcgt aacattcagg gatgtggcca tagaattctc ccctgaagag
       61 tggaaatgtc tggaccctgc ccagcagaat ttgtatagag atgtgatgtt ggagaactac
      121 aggaacctgg tctccctggc tatatgttct cctttcagcc aagacctttc accagtgcag
      181 gggatagaag attcattcca caaacttata ctgaaaagat acgagaaatg tggacatgag
      241 aatttacaat taagaaaagg ctgtaaacgt gtgaatgagt gtaaggtgca gaaaggagtt
      301 aataatggag tttaccagtg cttgtcaact acccagagca aaatatttca atgtaataca
      361 tgtgttaaag tttttagtaa attttcaaat tcaaacaaac ataagataag acatactgga
      421 gagaaaccct ttaaatgtac agaatgtggc agatcgtttt acatgtcaca cctaactcaa
      481 catacaggaa ttcatgctgg agagaaaccc tacaaatgtg aaaaatgtgg caaagccttt
      541 aataggtcca catcacttag taaacataag agaattcata ctggagagaa accctacaca
      601 tgtgaagaat gtggcaaagc ctttagacgg tccacagttc tgaacgaaca taagaaaatt
      661 catactggag agaaacccta caaatgtgaa gaatgtggca aagcctttac aaggtccaca
      721 acactgaatg aacacaagaa aattcatact ggagagaaac cctacaaatg taaagaatgt
      781 ggcaaagcct ttagatggtc cacaagcctg aatgaacata agaatattca tactggagag
      841 aaaccctaca aatgtaaaga atgtggcaaa gcctttagac agtccaggag cctgaatgaa
      901 cataaaaata ttcatactgg cgaaaaaccc tacacatgtg aaaaatgtgg caaagctttt
      961 aaccaatcct caagtcttat tatacacagg agcattcatt ctgaacaaaa actttacaaa
     1021 tgtgaagaat gtggcaaagc ctttacttgg tcctcatccc ttaataaaca taagagaatt
     1081 catactggag agaaacccta cacatgtgaa gaatgtggca aagcttttta taggtcctca
     1141 caccttgcta aacataagag aattcatact ggagagaaac cctacacgtg cgaagaatgt
     1201 ggcaaagctt ttaaccaatc ctcaactctt atattacaca agagaatcca ttctgggcaa
     1261 aaaccttaca aatgtgaaga atgtggcaaa gcctttacac ggtccacaac actgaacgaa
     1321 cataagaaaa ttcatactgg cgagaaaccc tacaaatgtg aagaatgtgg caaagctttc
     1381 atatggtccg caagcctgaa tgaacataag aatattcata ctggagagaa accctacaaa
     1441 tgtaaagaat gtggcaaagc ttttaaccaa tcctcaggcc ttattataca caggagcatt
     1501 cattctgaac aaaaacttta caaatgtgaa gaatgtggca aagcctttac tcggtccaca
     1561 gccctgaatg aacataagaa aattcattct ggagagaaac cctacaaatg caaagaatgt
     1621 ggcaaagcct ataacttatc ctcaaccctt actaaacata agagaattca tactggagag
     1681 aaacccttca catgtgaaga atgtggcaaa gccttcaatt ggtcctcatc ccttactaaa
     1741 cataagataa ttcatactgg agagaaatcc tacaaatgtg aagaatgtgg caaagctttt
     1801 aatcggccct caacccttac tgtacacaag cgaattcata ctggcaagga acatagttag
     1861 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1921 aaggacgatg tgtgacccgg cctcttcaaa ta
//
