LOCUS       sequence-283197-        2825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0322
FEATURES             Location/Qualifiers
     source          1..2825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtgacatt caaggatgtt gctgtggtct tcactgagga ggagctgggg
       61 ctgctggact ctgtccagag gaagctgtac cgagatgtga tgctggagaa cttcaggaac
      121 ctgctcttag tagcacatca gcccttcaag ccagacctaa tatcccagct ggagagagaa
      181 gaaaagcttt tgatggtgga gacagaaacc ccaagagatg gatgttcagg aaggaagaat
      241 caacaaaaga tggagagtat tcaggaagta acagtaagct acttttcccc caaagagctt
      301 tcctcccgtc agacctggca acaaagtgca ggtgggttaa tcaggtgtca agatttcctg
      361 aaagtttttc aagggaagaa ttctcagttg caagaacaag gtaattccct cggccaggtt
      421 tgggcaggaa taccagttca gatttctgaa gataagaact atatattcac tcatataggg
      481 aatggctcca attatataaa aagtcaaggg tatccatctt ggagggcaca tcattcttgg
      541 aggaaaatgt atctgaaaga gtcacataat tatcagtgta gatgtcagca aatttccatg
      601 aaaaatcatt tctgtaagtg tgacagtgtc agttggctct cacatcacaa tgataaactg
      661 gaagtacaca gaaaagaaaa ctacagctgc catgactgtg gagaagatat catgaaggta
      721 tcattactta atcaggagtc aattcaaaca gaggagaagc cctatccatg tactgggtat
      781 agaaaagcct tcagtaatga ctccagctct gaggttcatc agcagttcca cttggaaggg
      841 aagccctata catacagttc atgtggaaag ggctgtaatt atagttcact tcttcatatt
      901 catcaaaata ttgagagaga agatgatatt gagaattcac atctgaaatc ctatcagaga
      961 gtgcatacag aggagaaacc atgcaaatgt ggtgaatatg gtgagaactt caatcactgt
     1021 tcccctctta acacttatga acttatccac acaggtgaga tgtcctatag gcacaacatt
     1081 tatgagaaag ccttcagtca tagcttagac cttaatagta tttttagggt ccatactagg
     1141 gatgaacccc atgaatatga ggaaaatgag aatgtcttta atcagagttc atgtcttcaa
     1201 gtccatcaaa aaatccacac tgaagagaaa ctatacacag atatagagta tggaaagagt
     1261 ttcatttgta gttcaaatct tgacattcag catagggttc atatggaaga gaattcatat
     1321 aattctgagg agtgtggtaa tggcttcagt ctggcctcac attttcagga ccttcagata
     1381 gtccacacta aggaacaacc atataaacgc tatgtgtgta gtaacagctt cagccataat
     1441 ttatatcttc aaggtcatcc aaaaattcac attggagaga aaccacgtaa ggagcatggg
     1501 aatggcttca actggagctc aaaacttaaa gatcatcaga gagtccacac tggacagaag
     1561 ccatacaaat gcaatatatg cggcaaaggt ttcaatcata gatcagttct gaatgttcat
     1621 cagagagtcc acacaggaga gaaaccttat aaatgcgagg aatgtgataa gggattcagt
     1681 cggagttcat atcttcaagc ccatcagaga gtccacactg gagaaaaacc ttataaatgt
     1741 gaggaatgtg ggaaggggtt cagtcgaaat tcataccttc aaggccatca gagagttcac
     1801 actggagaaa aaccatacaa gtgtgaggag tgtgggaagg gcttcagtcg gagttcacac
     1861 cttcaaggcc atcagagagt ccacactgga gaaaaaccat tcaaatgtga ggaatgtggg
     1921 aaggggttca gttggagctt taatcttcaa attcatcaga gggttcacac aggagaaaaa
     1981 ccctataaat gtgaagaatg tggtaaaggc ttcagtaagg cctcaacact tttggcccat
     2041 cagagggtcc acacgggaga gaagccatac caatgtgatg agtgtggtaa gagtttcagt
     2101 cagagatcat accttcagag tcatcagagt gtccattctg gagaaagacc atatatatgt
     2161 gaggtatgtg gaaagggctt cagtcagaga gcatatcttc aaggtcatca gagagtccac
     2221 actagagtga aaccgtataa atgtgagatg tgtgggaagg gctttagtca gagttcgcgc
     2281 cttgaagcac atcggagggt tcacacagga gggaaaccat acaaatgtga ggtgtgtaca
     2341 aagggtttca gtgagagttc acgccttcaa gcacaccaaa gggttcatgt ggaagggaga
     2401 ccctataaat gtgaacagtg tggtaagggt ttcagtgggt attcaagtct tcaagcccat
     2461 cacagagtcc acacaggaga gaaaccatac aaatgtgagg tatgtggaaa gggcttcagt
     2521 cagagatcaa atcttcaggc tcaccagaga gtccacacag gagagaaacc atacaaatgt
     2581 gatgcatgtg gtaagggttt ccgttggagc tcaggtcttc tcattcatca aagagtccat
     2641 agtagtgata aattctataa aagcgaagac tatggtaagg actacccttc atcagagaat
     2701 ctacacagaa atgaagattc tgttttgttt tagtaatgaa ctagtccgta acttgaaagt
     2761 atttcgattt cttggcttta tatatcttgt ggaaaggacg acttcggaag gtctattggg
     2821 agatc
//
