LOCUS       sequence-283200-        2213 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0327
FEATURES             Location/Qualifiers
     source          1..2213
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1330..1341)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accgccacca tgtgtgtttt cttacatctc ctttcttaca taaaagatac cagtttcatt
       61 ccaggacctg actgtgaact tcacccaaga ggaatggcag caactggacc ctgctcagag
      121 gctcctgtac agggatgtga tgctggagaa ctacagcaac ttggtctctg tggagtctct
      181 gatgtccaaa gatcttggtc caagttctgt aatttcccat taacagggta tcatgttagc
      241 aaaccagatg tgattttcaa attggagcaa ggagaagagc catggatagt ggaggaattc
      301 tcaaatcaga actacccaga cattgatgat gccttagaga agaacaagga aatccaagat
      361 aaacatttga cacaaactgt attcttcagc aacaaaacac tgattacaga aagagagaat
      421 gtatttggga aaacacttaa tctgggcatg aatagtgttc cctcaagaaa aatgccctat
      481 aaatgtaatc caggaggaaa cagtttgaaa actaattcag aagtaattgt tgcaaagaaa
      541 agcaaagaaa acagaaagat tcctgatgga tacagtggat ttgggaagca tgagaaaagt
      601 catttgggaa tgaaaaaata cagatacaat ccaatgagga aagccagcaa tcaaaacgaa
      661 aatcttattc tgcaccagaa cattcagatt ttgaaacaac cgtttgacta taataaatgt
      721 gggaaaacct tcttcaagag ggcaattctc attacacaaa aggggagaca gactgaaagg
      781 aaaccaaatg aatgtaatga atgtaggaaa accttttcta agagatctac cctcattgta
      841 catcagagaa ttcatacagg ggagaaaccg tatgtttgta gtgattgtag gaaaactttt
      901 cgtgtgaaga caagcctcac tcgacaccga agaattcata ctggagagag accctatgaa
      961 tgcagtgaat gcaggaaaac cttcattgac aaatctgccc ttattgtaca ccagaaaatt
     1021 catggagggg agaaatccta tgagtgtaat gaatgtggaa agaccttttt tcggaagtca
     1081 gccctggctg aacatttcag gtcacacaca ggggagaagc cttacgaatg caaggaatgt
     1141 ggaaatgcct tcagcaagaa atcgtatctt gttgtacatc aaagaactca cagaggagag
     1201 aagccaaatg aatgtaagga atgtgggaaa accttcttct gtcagtcagc ccttactgcg
     1261 catcagagaa ttcacacagg ggaaaaaccc tatgaatgta gtgaatgtga gaaaaccttc
     1321 ttttgtcaat ctgccctcaa tgtgcatcga agaagtcata caggagagaa gccctatgaa
     1381 tgcagtcaat gtggaaaatt tttatgtacg aaatcagccc tcattgcaca tcagataact
     1441 catagaggaa agaagtctta tgaatgtaat gaatgtggga aatttttctg ccataagtca
     1501 acactcacta tacatcagag aacacacaca ggagagaaac atggtgtgtt taataaatgt
     1561 ggtagaatct ccattgtgaa gtcaaactgc agtcagtgta agagaatgaa cacaaaggag
     1621 aatctttatg agtgtagtga acatgggcat gccgtcagca aaaactcaca cctcattgta
     1681 catcagagaa ctatatggga gagaccatat gaatgcaatg aatgtgggag aacctactgc
     1741 aggaagtcag ccctgactca ccatcagaga acacacacag gacagagacc ctatgagtgt
     1801 aatgaatgtg ggaaaacctt ctgtcagaag ttctcctttg ttgaacatca gcgaactcac
     1861 actggggaga aaccatatga atgtaatgaa tgtgggaaat ccttctgcca taagtcagcc
     1921 ttcagagtcc atagaagaat tcacacagga gagaaaccat atgaatgtaa tcaatgtggg
     1981 aaaacctacc gtcgcctgtg gactctcact gaacatcaga aaatacacac aggagagaaa
     2041 ccttatgaat gtaacaaatg tgagaaaaca tttcgccaca aatcaaactt tcttttacat
     2101 cagaaatccc acaaggaata gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     2161 tggctttata tatcttgtgg aaaggacgaa cctcctcgaa cgatgcacag ttg
//
