LOCUS       sequence-283206-        2030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0329
FEATURES             Location/Qualifiers
     source          1..2030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1147..1158)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accgccacca tgctggagaa ctacagcaac ttggtctctg tggggtatca tgttagcaaa
       61 ccagatgtga ttttcaaatt ggagcaagga gaagagccat ggatagtgga ggaattctca
      121 aatcagaact acccagacat tgatgatgcc ttagagaaga acaaggaaat ccaagataaa
      181 catttgacac aaactgtatt cttcagcaac aaaacactga ttacagaaag agagaatgta
      241 tttgggaaaa cacttaatct gggcatgaat agtgttccct caagaaaaat gccctataaa
      301 tgtaatccag gaggaaacag tttgaaaact aattcagaag taattgttgc aaagaaaagc
      361 aaagaaaaca gaaagattcc tgatggatac agtggatttg ggaagcatga gaaaagtcat
      421 ttgggaatga aaaaatacag atacaatcca atgaggaaag ccagcaatca aaacgaaaat
      481 cttattctgc accagaacat tcagattttg aaacaaccgt ttgactataa taaatgtggg
      541 aaaaccttct tcaagagggc aattctcatt acacaaaagg ggagacagac tgaaaggaaa
      601 ccaaatgaat gtaatgaatg taggaaaacc ttttctaaga gatctaccct cattgtacat
      661 cagagaattc atacagggga gaaaccgtat gtttgtagtg attgtaggaa aacttttcgt
      721 gtgaagacaa gcctcactcg acaccgaaga attcatactg gagagagacc ctatgaatgc
      781 agtgaatgca ggaaaacctt cattgacaaa tctgccctta ttgtacacca gaaaattcat
      841 ggaggggaga aatcctatga gtgtaatgaa tgtggaaaga ccttttttcg gaagtcagcc
      901 ctggctgaac atttcaggtc acacacaggg gagaagcctt acgaatgcaa ggaatgtgga
      961 aatgccttca gcaagaaatc gtatcttgtt gtacatcaaa gaactcacag aggagagaag
     1021 ccaaatgaat gtaaggaatg tgggaaaacc ttcttctgtc agtcagccct tactgcgcat
     1081 cagagaattc acacagggga aaaaccctat gaatgtagtg aatgtgagaa aaccttcttt
     1141 tgtcaatctg ccctcaatgt gcatcgaaga agtcatacag gagagaagcc ctatgaatgc
     1201 agtcaatgtg gaaaattttt atgtacgaaa tcagccctca ttgcacatca gataactcat
     1261 agaggaaaga agtcttatga atgtaatgaa tgtgggaaat ttttctgcca taagtcaaca
     1321 ctcactatac atcagagaac acacacagga gagaaacatg gtgtgtttaa taaatgtggt
     1381 agaatctcca ttgtgaagtc aaactgcagt cagtgtaaga gaatgaacac aaaggagaat
     1441 ctttatgagt gtagtgaaca tgggcatgcc gtcagcaaaa actcacacct cattgtacat
     1501 cagagaacta tatgggagag accatatgaa tgcaatgaat gtgggagaac ctactgcagg
     1561 aagtcagccc tgactcacca tcagagaaca cacacaggac agagacccta tgagtgtaat
     1621 gaatgtggga aaaccttctg tcagaagttc tcctttgttg aacatcagcg aactcacact
     1681 ggggagaaac catatgaatg taatgaatgt gggaaatcct tctgccataa gtcagccttc
     1741 agagtccata gaagaattca cacaggagag aaaccatatg aatgtaatca atgtgggaaa
     1801 acctaccgtc gcctgtggac tctcactgaa catcagaaaa tacacacagg agagaaacct
     1861 tatgaatgta acaaatgtga gaaaacattt cgccacaaat caaactttct tttacatcag
     1921 aaatcccaca aggaatagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1981 ctttatatat cttgtggaaa ggacgaccac ggtaaccgca aagagcacgt
//
