LOCUS       sequence-283209-        2141 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0330
FEATURES             Location/Qualifiers
     source          1..2141
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1258..1269)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accgccacca tggaaaatga gaaggagata ccagtttcat tccaggacct gactgtgaac
       61 ttcacccaag aggaatggca gcaactggac cctgctcaga ggctcctgta cagggatgtg
      121 atgctggaga actacagcaa cttggtctct gtggggtatc atgttagcaa accagatgtg
      181 attttcaaat tggagcaagg agaagagcca tggatagtgg aggaattctc aaatcagaac
      241 tacccagaca ttgatgatgc cttagagaag aacaaggaaa tccaagataa acatttgaca
      301 caaactgtat tcttcagcaa caaaacactg attacagaaa gagagaatgt atttgggaaa
      361 acacttaatc tgggcatgaa tagtgttccc tcaagaaaaa tgccctataa atgtaatcca
      421 ggaggaaaca gtttgaaaac taattcagaa gtaattgttg caaagaaaag caaagaaaac
      481 agaaagattc ctgatggata cagtggattt gggaagcatg agaaaagtca tttgggaatg
      541 aaaaaataca gatacaatcc aatgaggaaa gccagcaatc aaaacgaaaa tcttattctg
      601 caccagaaca ttcagatttt gaaacaaccg tttgactata ataaatgtgg gaaaaccttc
      661 ttcaagaggg caattctcat tacacaaaag gggagacaga ctgaaaggaa accaaatgaa
      721 tgtaatgaat gtaggaaaac cttttctaag agatctaccc tcattgtaca tcagagaatt
      781 catacagggg agaaaccgta tgtttgtagt gattgtagga aaacttttcg tgtgaagaca
      841 agcctcactc gacaccgaag aattcatact ggagagagac cctatgaatg cagtgaatgc
      901 aggaaaacct tcattgacaa atctgccctt attgtacacc agaaaattca tggaggggag
      961 aaatcctatg agtgtaatga atgtggaaag accttttttc ggaagtcagc cctggctgaa
     1021 catttcaggt cacacacagg ggagaagcct tacgaatgca aggaatgtgg aaatgccttc
     1081 agcaagaaat cgtatcttgt tgtacatcaa agaactcaca gaggagagaa gccaaatgaa
     1141 tgtaaggaat gtgggaaaac cttcttctgt cagtcagccc ttactgcgca tcagagaatt
     1201 cacacagggg aaaaacccta tgaatgtagt gaatgtgaga aaaccttctt ttgtcaatct
     1261 gccctcaatg tgcatcgaag aagtcataca ggagagaagc cctatgaatg cagtcaatgt
     1321 ggaaaatttt tatgtacgaa atcagccctc attgcacatc agataactca tagaggaaag
     1381 aagtcttatg aatgtaatga atgtgggaaa tttttctgcc ataagtcaac actcactata
     1441 catcagagaa cacacacagg agagaaacat ggtgtgttta ataaatgtgg tagaatctcc
     1501 attgtgaagt caaactgcag tcagtgtaag agaatgaaca caaaggagaa tctttatgag
     1561 tgtagtgaac atgggcatgc cgtcagcaaa aactcacacc tcattgtaca tcagagaact
     1621 atatgggaga gaccatatga atgcaatgaa tgtgggagaa cctactgcag gaagtcagcc
     1681 ctgactcacc atcagagaac acacacagga cagagaccct atgagtgtaa tgaatgtggg
     1741 aaaaccttct gtcagaagtt ctcctttgtt gaacatcagc gaactcacac tggggagaaa
     1801 ccatatgaat gtaatgaatg tgggaaatcc ttctgccata agtcagcctt cagagtccat
     1861 agaagaattc acacaggaga gaaaccatat gaatgtaatc aatgtgggaa aacctaccgt
     1921 cgcctgtgga ctctcactga acatcagaaa atacacacag gagagaaacc ttatgaatgt
     1981 aacaaatgtg agaaaacatt tcgccacaaa tcaaactttc ttttacatca gaaatcccac
     2041 aaggaatagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     2101 tcttgtggaa aggacgactg tactaagttg gagtggtcga g
//
