LOCUS       sequence-283233-        2519 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0444
FEATURES             Location/Qualifiers
     source          1..2519
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatgaaga tgggcttgaa ttacaacaag agccaaactc attttttgat
       61 gcaacaggag ctgatggtac acacatggat ggtgatcaaa ttgttgtgga agtacaagaa
      121 actgtttttg tttcagatgt tgtggattca gacataactg tgcataactt tgttcctgat
      181 gacccagatt cagttgtaat ccaagatgtt attgaggacg ttgttataga agatgttcag
      241 tgcccagata tcatggaaga agcagatgtg tctgaaacgg tcatcattcc tgagcaagtg
      301 ctggactcag atgtaactga agaagtttct ttagcacatt gcacagtccc agatgatgtt
      361 ttagcttctg acattacttc agcctcaatg tctatgccag aacacgtctt gacgggtgat
      421 tctatacatg tgtctgacgt tggacatgtt ggacatgttg gacatgttga acatgtggtt
      481 catgatagtg tagtggaagc agaaattgtc actgatcctc tgactaccga cgtagtttca
      541 gaagaagtat tggtagcaga ctgtgcctct gaagcagtca tagatgccaa tgggatccct
      601 gtggaccagc aggatgatga caaaggcaac tgtgaggact accttatgat ttccttggat
      661 gatgctggca aaatagaaca cgatggttct tctggaatga ccatggacac agagtcggaa
      721 attgatcctt gtaaagtgga tggcacttgc cctgaggtca tcaaggtgta catttttaaa
      781 gctgaccctg gagaagatga cttaggtgga actgtagaca ttgtggagag tgagcctgag
      841 aatgatcatg gagttgaact gcttgatcag aacagcagta ttcgtgttcc cagggaaaag
      901 atggtttata tgactgtcaa tgactctcag ccagaagatg aagatttaaa tgttgctgaa
      961 atcgctgacg aagtttatat ggaagtgatc gtaggagagg aggatgctgc agcagcagcg
     1021 gcagccgccg ccgtgcacga gcagcaaatg gatgacaatg aaatcaaaac cttcatgccg
     1081 attgcatggg cagcagctta tggtaataat tctgatggaa ttgaaaaccg gaatggcact
     1141 gcaagtgccc tcttgcacat agatgagtct gctggcctcg gcagactggc taaacaaaaa
     1201 ccaaagaaaa ggagaagacc tgattccagg cagtaccaaa cagcaataat tattggccct
     1261 gatggacatc ctttgactgt ctatccttgc atgatttgtg ggaagaagtt taagtcgaga
     1321 ggttttttga aaaggcacat gaaaaaccat cccgaacacc ttgccaagaa gaaataccgc
     1381 tgtactgact gtgattacac taccaacaag aagataagtt tacacaacca cctggagagc
     1441 cacaagctga ccagcaaggc agagaaggcc attgaatgcg atgagtgtgg gaagcatttc
     1501 tctcatgcag gggctttgtt tactcacaaa atggtgcata aggaaaaagg agccaacaaa
     1561 atgcacaagt gtaaattctg tgaatacgag acagctgaac aagggttatt gaatcgccac
     1621 ctcttggcag tccacagcaa gaactttcct catatttgtg tggagtgtgg taagggtttt
     1681 cgtcacccgt cagagctcaa aaagcacatg agaatccata ctggggagaa gccgtaccaa
     1741 tgccagtact gcgaatatag gtctgcagac tcttctaact tgaaaacgca tgtcaaaact
     1801 aagcatagta aagagatgcc attcaagtgt gacatttgtc ttctgacttt ctcggatacc
     1861 aaagaggtgc agcaacatgc tcttatccac caagaaagca aaacacacca gtgtttgcat
     1921 tgcgaccaca agagttcgaa ctcaagtgat ttgaaacgac acataatttc agttcacacg
     1981 aaagactacc cccataagtg tgacatgtgt gataaaggct ttcacaggcc ttcagaactc
     2041 aagaaacacg tggctgccca caagggcaaa aaaatgcacc agtgtagaca ttgtgacttt
     2101 aagattgcag atccatttgt tctaagtcgc catattctct cagttcacac aaaggatctt
     2161 ccatttaggt gcaagagatg tagaaaggga tttaggcaac agagtgagct taaaaagcat
     2221 atgaagacac acagtggcag gaaagtgtat cagtgtgagt actgtgagta tagcactaca
     2281 gatgcctcag gctttaaacg gcacgttatt tccattcaca cgaaagacta tcctcaccgg
     2341 tgtgagtact gcaagaaagg cttccgaaga ccttcagaaa agaaccagca cataatgcga
     2401 catcataaag aagttggcct gccctagtaa tgaactagtc cgtaacttga aagtatttcg
     2461 atttcttggc tttatatatc ttgtggaaag gacgaacttg tataacgctt tgtcctctc
//
