LOCUS       sequence-283248-        1352 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0484
FEATURES             Location/Qualifiers
     source          1..1352
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagccat tctcctgacc acgagaccca aggtgccagt atcttttgag
       61 gatgtgtccg tgtacttcac aaagacagaa tggaagcttc tggacctcag acaaaaggtc
      121 ctctacaagc gggtgatgct ggagaactat agccatttgg tgtcactggg attttccttc
      181 tccaagcctc acctgatctc ccaattggaa cgaggggaag gaccctgggt agcagacatc
      241 cccagaacct gggccaccgc aggattgcac ataggtgaca gaacacagag caagacgtcg
      301 acttcaacgc agaagcattc tggacgacaa ctccccgggg ccgatccaca aggtggcaag
      361 gaggggcagg cggcgaggtc gtctgtgctc cagagaggtg cccagggctt ggggcagagt
      421 tcggctgcgg ggccgcaggg ccccaaaggc gcggagaagc ggtacctgtg ccagcagtgt
      481 gggaaggcct tcagccgcag ctccaacctc atcaagcacc gcatcatcca cagtggcgag
      541 aagccttacg cgtgccccga gtgcggcaag ctgtttcgcc gcagcttcgc gctcctggag
      601 caccagcgca tccacagcgg cgagaagccc tacgcctgcc ccgagtgcag caagaccttc
      661 acgcgcagct ccaacctcat caagcaccag gtcatccaca gcggcgagcg gcccttcgcc
      721 tgcggcgact gcggcaaact gttccgccgc agcttcgcgc tcctggagca cgcgcgcgtg
      781 cacagcggcg agcggcccta cgcgtgccca gagtgcggca aggccttcag ccgcagctcc
      841 aacctcatcg agcaccagcg cacgcaccgc ggcgagaagc cctacgcctg cggccagtgc
      901 gccaaggcct tcaagggcgt ctcgcagctc atccaccacc agcgcagcca cagcggcgag
      961 cggcccttcg cgtgccgcga gtgcggcaag gccttccgtg gccgttcggg cctcagccag
     1021 caccggcgcg tgcacagcgg tgagaagccc tacgagtgca gcgactgcgg caaggccttc
     1081 ggccggcgcg ccaacctatt caagcaccag gcagtgcacg gcgccaggcg ccctgcgaag
     1141 gcggagacgg cgcggaggct agcgggccct gggagcaccg gccctgggag cgcggtggcg
     1201 gccaccagcc ccccgcggcc gagcacagcc gccaggcctt ccaggcccag ccgccgctag
     1261 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1321 aaggacgaca ccagcacgct tgcaagatcc ga
//
