LOCUS       sequence-283254-        1151 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0498
FEATURES             Location/Qualifiers
     source          1..1151
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagccatg tgcggcgtgg agtccccgcg gtgggagaga gaatgtaatg
       61 gacatcggtc tgaccaacga gaagcccaac ccggaactct cttactccgg ctccttccag
      121 ccagcccccg gcaacaagac cgtgacctac ttgggaaagt tcgccttcga ctccccttcc
      181 aactggtgcc aggacaacat cattagcctc atgagcgccg gcatcttggg ggtgcccccg
      241 gcttcagggg cgctcagcac gcagacgtcc acggccagca tggtgcagcc accgcagggt
      301 gacgtggagg ccatgtatcc cgcgctaccc ccctactcca actgcggcga cctctactca
      361 gagcccgtgt ctttccacga cccccagggc aatcccgggc tcgcctattc cccccaggat
      421 taccaatcgg ccaagccggc gttggacagc aatctcttcc ccatgattcc tgactacaac
      481 ctctaccacc accccaacga catgggctcc attccggagc acaagccctt ccagggcatg
      541 gaccccatcc gggtcaaccc gccccctatt acccctctgg agaccatcaa ggcattcaaa
      601 gacaagcaga tccacccggg ctttggcagc ctgccccagc cgccgctcac cctcaagccc
      661 atccggcccc gcaagtaccc caaccggcct agcaagacac cgctccacga acggccccac
      721 gcgtgcccgg ccgagggctg cgaccgccgt ttcagccgtt cggacgagct gacccggcac
      781 ctgcgcatcc acacgggcca caagcccttc cagtgccgga tctgcatgcg gagcttcagc
      841 cgcagcgacc acctcaccac tcacatccgc actcatacgg gcgagaagcc ctttgcctgc
      901 gagttctgcg ggcgcaagtt tgcgcgcagc gacgagcgca agcgccacgc caagatccac
      961 ctcaagcaaa aggagaagaa ggcggagaag ggcggtgcac cctctgcatc ctcggcgccc
     1021 cccgtgtcgc tggcccccgt ggtcaccacc tgcgcctagt aatgaactag tccgtaactt
     1081 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgagca aacatgtgta
     1141 ggtgtagtga g
//
