LOCUS       sequence-283257-        2498 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0500
FEATURES             Location/Qualifiers
     source          1..2498
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgtggga catgtgcaac caggactctg agtctgtatg gagtgacatc
       61 gagtgtgctg ctctggttgg tgaagaccag cctctttgcc cagatcttcc tgaacttgat
      121 ctttctgaac tagatgtgaa cgacttggat acagacagct ttctgggtgg actcaagtgg
      181 tgcagtgacc aatcagaaat aatatccaat cagtacaaca atgagccttc aaacatattt
      241 gagaagatag atgaagagaa tgaggcaaac ttgctagcag tcctcacaga gacactagac
      301 agtctccctg tggatgaaga cggattgccc tcatttgatg cgctgacaga tggagacgtg
      361 accactgaca atgaggctag tccttcctcc atgcctgacg gcacccctcc accccaggag
      421 gcagaagagc cgtctctact taagaagctc ttactggcac cagccaacac tcagctaagt
      481 tataatgaat gcagtggtct cagtacccag aaccatgcaa atcacaatca caggatcaga
      541 acaaaccctg caattgttaa gactgagaat tcatggagca ataaagcgaa gagtatttgt
      601 caacagcaaa agccacaaag acgtccctgc tcggagcttc tcaaatatct gaccacaaac
      661 gatgaccctc ctcacaccaa acccacagag aacagaaaca gcagcagaga caaatgcacc
      721 tccaaaaaga agtcccacac acagtcgcag tcacaacact tacaagccaa accaacaact
      781 ttatctcttc ctctgacccc agagtcacca aatgacccca agggttcccc atttgagaac
      841 aagactattg aacgcacctt aagtgtggaa ctctctggaa ctgcaggcct aactccaccc
      901 accactcctc ctcataaagc caaccaagat aaccctttta gggcttctcc aaagctgaag
      961 tcctcttgca agactgtggt gccaccacca tcaaagaagc ccaggtacag tgagtcttct
     1021 ggtacacaag gcaataactc caccaagaaa gggccggagc aatccgagtt gtatgcacaa
     1081 ctcagcaagt cctcagtcct cactggtgga cacgaggaaa ggaagaccaa gcggcccagt
     1141 ctgcggctgt ttggtgacca tgactattgc cagtcaatta attccaaaac agaaatactc
     1201 attaatatat cacaggagct ccaagactct agacaactag aaaataaaga tgtctcctct
     1261 gattggcagg ggcagatttg ttcttccaca gattcagacc agtgctacct gagagagact
     1321 ttggaggcaa gcaagcaggt ctctccttgc agcacaagaa aacagctcca agaccaggaa
     1381 atccgagccg agctgaacaa gcacttcggt catcccagtc aagctgtttt tgacgacgaa
     1441 gcagacaaga ccggtgaact gagggacagt gatttcagta atgaacaatt ctccaaacta
     1501 cctatgttta taaattcagg actagccatg gatggcctgt ttgatgacag cgaagatgaa
     1561 agtgataaac tgagctaccc ttgggatggc acgcaatcct attcattgtt caatgtgtct
     1621 ccttcttgtt cttcttttaa ctctccatgt agagattctg tgtcaccacc caaatcctta
     1681 ttttctcaaa gaccccaaag gatgcgctct cgttcaaggt ccttttctcg acacaggtcg
     1741 tgttcccgat caccatattc caggtcaaga tcaaggtctc caggcagtag atcctcttca
     1801 agatcctgct attactatga gtcaagccac tacagacacc gcacgcaccg aaattctccc
     1861 ttgtatgtga gatcacgttc aagatcgccc tacagccgtc ggcccaggta tgacagctac
     1921 gaggaatatc agcacgagag gctgaagagg gaagaatatc gcagagagta tgagaagcga
     1981 gagtctgaga gggccaagca aagggagagg cagaggcaga aggcaattga agagcgccgt
     2041 gtgatttatg tcggtaaaat cagacctgac acaacacgga cagaactgag ggaccgtttt
     2101 gaagtttttg gtgaaattga ggagtgcaca gtaaatctgc gggatgatgg agacagctat
     2161 ggtttcatta cctaccgtta tacctgtgat gcttttgctg ctcttgaaaa tggatacact
     2221 ttgcgcaggt caaacgaaac tgactttgag ctgtactttt gtggacgcaa gcaatttttc
     2281 aagtctaact atgcagacct agattcaaac tcagatgact ttgaccctgc ttccaccaag
     2341 agcaagtatg actctctgga ttttgatagt ttactgaaag aagctcagag aagcttgcgc
     2401 aggtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     2461 tgtggaaagg acgattcccg gagtgtgagg cgcattgg
//
