LOCUS       sequence-283266-        2447 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0561
FEATURES             Location/Qualifiers
     source          1..2447
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctactca ggggcatttg acattcaagg atgtagccat agaattctct
       61 caggaggagt ggaaatgcct ggagcctgtg cagaaagctt tgtacaagga tgtgatgttg
      121 gagaactata ggaacctggt cttcctaggt atctctccta aatgtgtgat caaggaatta
      181 ccaccaacag agaacagtaa tacaggagaa aggttccaaa cagtggcact ggaaagacat
      241 caaagctatg atattgaaaa tttatacttc agggaaatac agaaacatct acatgacctt
      301 gaatttcaat ggaaagatgg tgaaacaaat gataaagaag tgccagtgcc ccatgaaaac
      361 aatcttactg gtaaaagaga tcaacatagt caaggggatg tagaaaacaa tcatattgaa
      421 aaccagctta catcaaactt tgagtcacgt ctggctgaac tgcagaaagt tcaaactgaa
      481 gggagacttt atgaatgtaa tgaaacggag aagacaggta ataatggttg tttagtttct
      541 ccacacatta gggaaaaaac gtatgtatgt aatgaatgtg gcaaagcctt taaagcgtct
      601 tccagcctta ttaatcatca gaggatacat actacagaga aaccttacaa atgcaatgag
      661 tgtggcaaag cctttcatcg ggcctcacta ctaactgtac acaaggtagt ccatacaaga
      721 gggaaatcat atcaatgtga tgtatgtggc aagatcttca gaaaaaattc atattttgta
      781 agacaccaaa ggagtcacac tggacagaaa ccctacatat gtaatgaatg tggcaagtcc
      841 tttagtaaaa gttcccacct tgcagttcat cagagaattc ataccggtga aaaaccttac
      901 aaatgtaatc tgtgtgggaa atcctttagt cagcgtgtcc atcttagact tcatcagaca
      961 gttcatactg gagagagacc cttcaaatgt aatgagtgtg gcaaaacctt taaacggagc
     1021 tcaaacctca ctgtacatca ggtaatccat gcaggaaaga aaccatataa atgtgatgta
     1081 tgtggcaagg cattcagaca tagatcaaat cttgtatgtc accggagaat ccacagtgga
     1141 gagaaacaat acaaatgcaa tgaatgtggc aaggtcttca gtaaacgttc aagtcttgca
     1201 gtgcatcgac gaattcacac tgtagagaaa ccttgcaaat gcaatgaatg tggcaaggtc
     1261 ttcagtaaac gttcaagtct tgcagtgcat cagagaattc atactggaca gaaaacttac
     1321 aaatgcaata aatgtggcaa ggtgtacagt aagcattcac atcttgcagt gcattggaga
     1381 attcataccg gcgagaaagc ttataaatgc aatgaatgtg gcaaagtttt cagcatacat
     1441 tcacgacttg cagctcatca gagaattcat actggagaga aaccttacaa atgcaatgaa
     1501 tgtggcaagg tcttcagtca acattcacgt cttgcagtgc atcggagaat tcatactgga
     1561 gagaaacctt acaaatgcaa agaatgtggc aaggtcttca gtgaccgttc agcttttgca
     1621 aggcatcgga gaattcatac tggagagaag ccttacaaat gcaaagaatg tggcaaggtc
     1681 ttcagtcaat gttcacgtct tacagtgcat cggagaattc atagtggaga gaaaccttac
     1741 aaatgcaatg aatgcggcaa ggtctacagt cagtattcac atcttgtagg gcatcgaaga
     1801 gttcatactg gagagaaacc atacaaatgt catgaatgtg gcaaagcctt taatcagggc
     1861 tccacactca atagacatca gagaattcat accggagaga aaccttacaa atgcaatcag
     1921 tgtgggaatt cctttagtca gcgtgtccat cttagacttc atcagactgt tcatactgga
     1981 gacagacctt acaaatgtaa tgagtgtggc aaaaccttta aacggagctc aaacctcact
     2041 gcacatcaga taattcatgc aggaaagaaa ccatataaat gtgatgaatg tggcaaggta
     2101 ttcaggcata gttcacatct tgtaagtcac cagagaatcc acactggaga gaaaagatac
     2161 aaatgtattg aatgtggcaa agcctttggg cggttgtttt ccctcagcaa acaccaaaga
     2221 attcattctg gcaaaaaacc ttataaatgt aatgagtgtg ggaaatcttt tatttgtcgc
     2281 tcaggcctca ctaaacatcg aataagacat actggagaga gccttacaac taaactcaat
     2341 gtgacaaggc cttagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     2401 tatatatctt gtggaaagga cgagtggcct catctgtagc atcgtgt
//
