LOCUS       sequence-283268-       18058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18058)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 18058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0569
FEATURES             Location/Qualifiers
     source          1..18058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             complement(12068..12079)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     misc_feature    13915..14503
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(13968..13988)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(14386..14397)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             14575..14808
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    14886..15007
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14923..14942)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14977..14996
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      15047..15182
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(15202..15221)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(15203..15221)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(15231..15248)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(15231..15247)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15240..15262)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      15389..15844
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15476..15495)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15686..15707
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        15870..15974
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(15975..16043,16044..16835)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(16193..16212)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      17006..17594
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     17495..17514
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     17748..17765
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    17882..17903
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(17918..17923,17924..17941,17942..17948)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    17956..17972
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     17961..17983
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     17980..17996
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     17980..17996
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     18015..18035
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        18017..18035
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgcag agcagcccct cccctgctca
     4141 ccctcagctc ccagtcctac agacacagat ggtgtcggac ggcatgacag gcagcaatcc
     4201 tgtgtcccct gcctcatcca gttccccagc ctctagtggg gcaggcggca tctccccgca
     4261 gcacatagct caagattcct cactggatgg acctccaggc cccccagatg gtgccacagt
     4321 gcccctggag gggttcagct tatcccaggc tgctgacctg gctaacaagg gcccgaagtg
     4381 ggagaagagc catgccgaaa ttgcagaaca ggccaagcat gaggccgaga tcgagactcg
     4441 gattgctgag ctgcggaagg agggtttctg gtcactgaag aggctgccta aggtgccaga
     4501 gccccctcgc cccaaaggtc actgggacta tttgtgcgaa gagatgcagt ggctctctgc
     4561 tgactttgct caggagcgcc gttggaaacg gggtgtggcc cggaaggtgg tgcgcatggt
     4621 gatccggcac cacgaggagc agcggcagaa agaggaacgg gcccggaggg aggagcaggc
     4681 caagctgcgt cgaattgctt ccaccatggc caaggatgtc aggcagttct ggagcaatgt
     4741 ggagaaggtg gtgcaattca agcaacagtc ccggcttgag gaaaagcgca aaaaagccct
     4801 ggacctgcat ttggacttca ttgtggggca aactgaaaag tactcggacc ttctgtctca
     4861 gagcctcaac cagccattaa cctccagcaa agcaggctct tccccttgcc tcggctcttc
     4921 ctcagctgcc tccagtcctc caccccctgc ttctcgcctg gatgatgaag atggggactt
     4981 tcaaccccaa gaggatgagg aagaggatga tgaggaaacg attgaagttg aagaacaaca
     5041 ggaaggcaat gatgcagagg cccagaggcg tgagattgag ctgcttcgcc gtgagggaga
     5101 attgccactg gaagagctgc tccgttccct tccccctcag ctgttggaag ggccttccag
     5161 cccctctcaa accccctcat ctcatgatag tgacacccga gatgggcctg aagaaggtgc
     5221 tgaagaagag ccccctcagg tgttggagat aaagccccca ccctctgctg tcacacagcg
     5281 caacaaacag ccttggcatc cagatgaaga tgatgaagag tttactgcca acgaagagga
     5341 agcggaggat gaagaggata ctatagcagc tgaggaacag ttggaagggg aggtggatca
     5401 tgccatggag ctgagcgagt tggctcgaga aggtgagctt tccatggagg agctattgca
     5461 gcagtatgca ggagcctatg ccccaggctc tgggagcagt gaagatgagg atgaagatga
     5521 ggttgatgct aatagctctg actgtgaacc agaggggccc gtggaagcgg aagagcctcc
     5581 tcaggaggat agtagcagtc agtcagactc tgtggaggac cggagtgagg atgaggaaga
     5641 tgaacattca gaggaggaag aaacaagtgg aagttcagca tcagaggaat ctgagtctga
     5701 agagtctgag gatgcccaat cacagagcca agcagatgaa gaggaggaag atgatgattt
     5761 tggggtggag tacttgcttg ccagggatga agagcagagt gaggcagatg caggcagtgg
     5821 gcctcctact ccagggccca ctactctagg tccaaagaaa gaaattactg acattgctgc
     5881 agcagctgaa agtctccagc ccaagggtta cacgctggcc acgacccagg taaagacgcc
     5941 cattcccctg cttctgcggg gccagctccg ggagtaccag cacattgggc tagactggct
     6001 ggttaccatg tatgagaaga agcttaatgg cattcttgct gatgagatgg ggcttgggaa
     6061 gaccatccag accatctctc tgcttgccca cttggcttgt gagaaaggta actggggtcc
     6121 ccatttaatc attgttccca ccagcgtgat gttgaactgg gagatggagt tgaaacggtg
     6181 gtgccccagc tttaaaatcc tcacttacta tggagcccag aaagagagga agctcaagcg
     6241 gcagggctgg accaagccca atgcctttca tgtgtgtatc acatcttaca agctggtgct
     6301 gcaggaccac caggccttcc gtcgcaagaa ctggcgctat ctcattctgg atgaggcgca
     6361 gaacatcaag aacttcaagt cacagcgctg gcagtcactc ctcaacttca acagccagag
     6421 acgcctgctc ctgacaggaa ctcccttgca gaacagcctc atggagctgt ggtccttgat
     6481 gcactttttg atgccccatg tcttccagtc tcatcgcgag ttcaaggagt ggttctctaa
     6541 tcccctaact ggcatgattg agggcagcca agagtataat gaaggtctag tcaaacgcct
     6601 ccacaaggtt ttgaggcctt ttttactgcg ccgagttaag gtggatgttg agaagcagat
     6661 gcccaaaaag tacgagcatg ttatccgctg caggctctcc aagcgtcaac gctgtctcta
     6721 tgatgacttc atggcacaga ccacaactaa ggagacacta gccacaggcc atttcatgag
     6781 cgtcatcaac attttgatgc agctgagaaa agtttgcaat catccaaatc tgttcgaccc
     6841 tcgaccggtt acctcccctt tcatcacccc aggcatctgc ttcagcaccg cctctctggt
     6901 gctaagggcc acggatgtcc atcccctcca gcggatagac atgggtcgat ttgaccttat
     6961 tggcctggaa ggtcgtgtct ctcgatatga ggcagacaca tttctgcccc ggcaccgcct
     7021 ctctcgccgg gtactgttag aagtggctac tgctcctgac cccccacccc ggcccaagcc
     7081 agtcaagatg aaggtcaaca ggatgctgca gccagtacct aagcaagaag gccggacagt
     7141 ggtggtggtg aacaacccac gggcgcccct gggccctgtc ccagttcgac ctcctccagg
     7201 tcctgagctc tcagcccagc ccacccctgg cccagtcccc caagtgctgc cagcatcact
     7261 gatggtttca gcctcacctg ccgggccccc gcttattcct gcatctcggc ctcctggccc
     7321 tgtcctcttg cctccactgc agcccaacag tggttctctc ccccaggtgt tgccatcccc
     7381 cctgggggtc ctgagtggga cctcacggcc tcccacgcca accttgtccc taaagccaac
     7441 accacctgcc ccagttcgcc tgagcccagc cccacctcca ggctcctcta gcctgttgaa
     7501 gcccctgaca gtgccaccag gctacacctt ccctcctgct gctgccacca ccacttctac
     7561 caccacggca actgctacca ccacagcagt gccagctccg actcctgcac cacagcgcct
     7621 cattctatct cccgatatgc aggctcgcct gccctcaggc gaagtggtca gcatcgggca
     7681 gttagcctca ctggcacaac gtccagtggc taatgcaggg ggaagcaaac ctctcacctt
     7741 ccaaatccag ggcaacaagc tgactttgac tggtgcccag gtgcgccagc ttgctgtggg
     7801 gcagccccgc ccgctgcaaa ggaatgtggt gcacctcgtg tcagcagggg ggcagcacca
     7861 tctcatcagc cagcctgccc atgtggccct catccaggcc gtggccccga cccctggccc
     7921 tacccctgtc tctgtgctgc cttcttcgac ccccagcacc acccctgccc ctactggcct
     7981 cagccttccg cttgctgcta accaggtgcc accaaccatg gtgaataata caggcgtggt
     8041 gaagattgta gtgagacaag cccctcggga tggactgact cctgttcctc cattggcccc
     8101 agcaccccgg cctccgagct ctgggcttcc agctgtgttg aatccacgcc ccacgttaac
     8161 ccctggccgg ctacccacac ctactctggg tactgctcga gcccccatgc ccacacccac
     8221 tctggtgagg cctcttctca agctggtcca cagtccttca cctgaagtca gtgcttcagc
     8281 ccccggagct gcccccttga ccatctcttc tcctctccac gtgccatcct ccctccctgg
     8341 gccagcctct tctccaatgc caattcccaa ctcctctccc cttgctagtc ctgtgtcctc
     8401 tacagtctca gttccattgt catcttcact ccccatctct gtccccacca cacttcctgc
     8461 cccagcctcg gctccactca ccatccccat ctcagccccc ttgactgttt ctgcttcggg
     8521 cccagctctg ttgaccagtg tgactccacc attggcacct gttgtcccag cggctcctgg
     8581 acctccctcc ttggcaccat ctggtgcttc cccgtcagca tcagccttga ctctaggttt
     8641 ggccacagct ccatccctgt cttcatctca gacacctggt caccctctgt tgttggctcc
     8701 cacctcttca catgttccag ggttgaactc aaccgtggcc ccagcatgct cacctgtcct
     8761 ggtgccagct tcggctctgg ccagtccttt tccgtcagca ccaaatccag ctccagctca
     8821 ggcttccctt ctggctccag catcttctgc atctcaggct ctagccaccc ctctggctcc
     8881 tatggcggct ccacagacag caattctggc tccttctcca gctcctcctc tggctcctct
     8941 tccggtcctg gcaccatcgc caggtgctgc tcctgtcctg gcttcatcac agactccggt
     9001 tccagttatg gctccatcgt ctactccagg aacctcttta gcctcagctt caccggtacc
     9061 agctccaacc cctgtgttgg ctccatcatc aactcaaact atgctaccag ccccggttcc
     9121 gtcacctctc ccgagcccgg cttctacgca gacactggcc ctagccccag ctttagcacc
     9181 cactcttgga ggctcatctc catctcagac actctctttg ggaacgggga acccccaggg
     9241 accctttcca actcagacat tgtcattaac tccagcatca tccctggtac caactccagc
     9301 ccagacactg tctttggcac caggaccacc actgggtcca actcagacgc tgtctctggc
     9361 tccagcaccc cctctggctc cagcttctcc agtgggccca gccccagctc acacgctgac
     9421 tttggctcca gcatcgtcat ctgcttcact cctggcccca gcttcagtgc agacactgac
     9481 cttgagccct gccccagttc ctaccctggg cccggccgca gctcagacct tggcgctggc
     9541 cccagcctcc acacagtccc cagcttccca ggcatcttcc cttgtggttt cggcatctgg
     9601 tgccgctccc ttgcctgtca ccatggtatc ccggctgcct gtttccaagg atgagcctga
     9661 cacactgaca ttgcgctctg gtccccccag ccctccctcc actgctacct cgtttggtgg
     9721 cccccggcct cgacgccagc cccccccacc acctcgttcc cctttttatc tggactccct
     9781 ggaggaaaag cggaagcggc agcggtctga acgcctggaa cggattttcc aacttagtga
     9841 ggctcatggg gccctggcac ctgtgtatgg gactgaagtc ctggatttct gtaccctgcc
     9901 ccaacctgtt gccagcccca tcggccctcg ttctcctggc cccagccacc ccaccttttg
     9961 gacttatacc gaggctgccc accgggctgt actgtttccc cagcagcgac tagaccagct
    10021 gtcagaaatc attgagaggt tcatctttgt catgcctcct gtggaggcac ctcccccttc
    10081 cctgcatgcc tgccacccac ctccttggct ggccccacgt caggcagcct tccaggagca
    10141 attggcctct gagctctggc cccgggctcg tcctttgcac cgtattgtgt gtaacatgcg
    10201 cacccagttc cctgacttaa gactcatcca gtatgattgc ggaaagttgc agacgttggc
    10261 agtgctgttg cggcagctca aggcagaggg ccaccgagtg ctcatcttca cccagatgac
    10321 ccgaatgctg gatgtattgg agcagtttct cacctaccat ggccatctct acctgcgcct
    10381 ggatggatct actagagttg aacagagaca ggccttgatg gaacggttca atgcagacaa
    10441 acgcatattc tgcttcatcc tttcaactcg gagtgggggt gtgggcgtga acctgacagg
    10501 agcagacact gttgtttttt atgacagcga ctggaatccc accatggatg ctcaggccca
    10561 ggaccgctgt caccgaattg gccagacccg ggatgtccac atatataggc ttatcagtga
    10621 acggacagtg gaggagaaca tcctaaaaaa ggcaaatcag aagagaatgt tgggggacat
    10681 ggccattgag ggaggcaact tcaccacagc ctatttcaaa cagcagacca tccgagagct
    10741 gtttgatatg cccctggagg aaccttctag ctcatccgtg ccctctgccc ctgaagagga
    10801 ggaagagact gtggccagca agcagactca tattctggag caggcattgt gtcgggcaga
    10861 agatgaagag gatatccgtg cagccaccca ggccaaggct gaacaggtgg ctgagcttgc
    10921 agaatttaat gagaacgatg ggtttcctgc tggtgaggga gaggaagctg gccggcctgg
    10981 ggctgaggat gaggagatgt cccgggctga gcaggaaatt gctgccctcg tagaacagct
    11041 gacccccatt gagcgctatg ccatgaaatt cctggaggcc tcactggagg aggtgagccg
    11101 agaggagctc aaacaggcag aagagcaagt ggaagctgcc cgcaaagacc tggaccaagc
    11161 caaggaggag gtgttccgcc taccccaaga ggaggaggag gggccggggg ctggggatga
    11221 gagttcctgt gggactggtg gaggcaccca ccggcgcagt aaaaaggcca aagcccctga
    11281 gaggccgggg actcgtgtca gtgagcgtct tcgtggagcc cgggctgaga ctcaaggggc
    11341 aaaccacact cctgtcatat ccgcccatca aactcgcagc accaccacac caccccgctg
    11401 cagtcctgcc agggagcgag ttcccaggcc agcacctagg cctcgaccca ctccagcttc
    11461 agctccggct gcaattcctg cccttgttcc tgtcccagtt tctgccccag tacccatttc
    11521 agccccaaat ccaataacca ttctccctgt ccatatcttg ccttctcctc cccctccttc
    11581 acagattcct ccttgttctt ctcctgcctg cacccctcct cctgcctgta cccctccacc
    11641 agctcataca ccgcctccag cccaaacctg tcttgtaact ccttcctctc ctctcttgct
    11701 tggtccacct tctgtgccca tctctgcctc agtcactaat ctccccttgg gcttgaggcc
    11761 tgaggcagag ctgtgtgccc aggcattggc atctccagag tccctggagc tggcttctgt
    11821 ggccagttca gaaacctcct cactttctct tgtgccccct aaagatctgt tgccagttgc
    11881 tgtggagatc ctgcctgtgt cagagaagaa cctttctctc accccttctg cacccagcct
    11941 gaccttggag gctggcagca tccccaatgg tcaagagcag gaggcaccag attctgctga
    12001 ggggaccacc cttacagtgc tgcctgaagg tgaggagttg cccctgtgtg tgagtgagag
    12061 caatggcctg gagctcccac cctcagcagc atctgatgag ccacttcagg agccactgga
    12121 ggctgacagg acctcggaag agctgacaga ggccaagacc ccaacctcca gcccagagaa
    12181 gccacaggaa ctcgttacag ctgaggttgc agctccatcc acctcatctt cagccacttc
    12241 ctcgcctgag ggtccttcac ctgcccgacc tcctcggcgt cgcaccagtg ctgatgtgga
    12301 aattaggggt caagggactg gtcggccagg acaaccacca ggccccaaag tgcttcgaaa
    12361 gctgccagga cggctggtaa ctgtggtaga ggaaaaggaa ctggtgcggc ggcggcggca
    12421 gcagcgggga gctgccagca ccctagtgcc tggggtctct gagactagtg ccagcccggg
    12481 aagcccgtct gtccgcagca tgtcagggcc agaatcctcc cctcccattg gtgggccctg
    12541 tgaagctgct ccttcatcct cactgcccac tccaccccag cagcccttca ttgctcgccg
    12601 tcacattgag ctgggggtga ctggtggtgg cagccccgag aatggagacg gagcactgct
    12661 cgccatcacc ccacctgctg tgaaacgtcg gagggggagg ccccccaaga agaacaggtc
    12721 tccagcagat gctgggagag gtgtggatga ggcaccctca tccaccttga agggaaaaac
    12781 caatggggct gacccagtcc ctgggcctga gaccctaatt gttgcagatc ctgtcctgga
    12841 accacagctt attcctgggc cccagcctct tggaccccag ccagttcaca gacccaatcc
    12901 cctcctgtca cctgtggaga aaagaaggcg aggacgaccc cctaaagcac gagatttgcc
    12961 catccctggg accatttcct ctgcagggga tggcaactcc gaaagtcgga cacagccacc
    13021 cccacaccca tcacccctaa ccccactccc accactgcta gtttgtccca ctgctactgt
    13081 tgccaacact gtcaccactg tcaccatttc aacgtcccca cccaaacgga agaggggccg
    13141 acctcccaag aatcctccat cacctcggcc cagccagctc cccgtcttgg accgtgacag
    13201 cacttctgtt ctcgagagct gtggattggg gaggcgacgg caaccccagg gccaagggga
    13261 gagtgagggt agttcctctg atgaggatgg aagccgcccc ctcacccgcc tggcccgcct
    13321 tcggcttgaa gcagaaggaa tgcgaggacg gaagagtgga gggtccatgg tggtggctgt
    13381 aattcaggat gacctggact tagcagatag cgggccaggc gggttggaat tgacaccacc
    13441 tgtggtctca ctaaccccaa aactgcgctc gacccggctg cgtccagggt ctctagtccc
    13501 cccactagag actgagaagt tgcctcgcaa acgagcaggg gccccagttg gtgggagtcc
    13561 tgggctggca aagcggggcc gcctacagcc cccaagtccc ctggggcctg agggttcagt
    13621 agaggagtct gaggctgaag cctcaggtga ggaggaggaa ggggatggga ccccacgccg
    13681 acgtcctggc ccccgccggc ttgttgggac caccaaccaa ggggaccagc gcatcctgcg
    13741 cagcagcgcc cctccctccc tggctggccc tgctgttagt cacagaggcc gcaaggccaa
    13801 gacgtagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
    13861 ttgtggaaag gacgataaag taaacaactt ggaactagaa cgcgttaagt cgacaatcaa
    13921 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
    13981 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
    14041 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
    14101 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
    14161 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
    14221 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
    14281 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
    14341 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
    14401 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
    14461 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
    14521 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
    14581 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
    14641 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
    14701 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
    14761 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
    14821 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
    14881 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
    14941 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    15001 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
    15061 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
    15121 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    15181 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
    15241 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
    15301 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
    15361 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
    15421 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
    15481 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
    15541 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
    15601 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
    15661 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
    15721 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
    15781 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
    15841 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
    15901 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    15961 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
    16021 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
    16081 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
    16141 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
    16201 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
    16261 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    16321 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    16381 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    16441 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    16501 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
    16561 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    16621 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    16681 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    16741 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    16801 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    16861 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    16921 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    16981 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    17041 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    17101 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    17161 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    17221 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    17281 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    17341 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    17401 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    17461 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    17521 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    17581 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    17641 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    17701 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    17761 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    17821 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    17881 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    17941 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    18001 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagc
//
