LOCUS       sequence-283269-        9794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0569
FEATURES             Location/Qualifiers
     source          1..9794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7963..7974)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accgccacca tgcagagcag cccctcccct gctcaccctc agctcccagt cctacagaca
       61 cagatggtgt cggacggcat gacaggcagc aatcctgtgt cccctgcctc atccagttcc
      121 ccagcctcta gtggggcagg cggcatctcc ccgcagcaca tagctcaaga ttcctcactg
      181 gatggacctc caggcccccc agatggtgcc acagtgcccc tggaggggtt cagcttatcc
      241 caggctgctg acctggctaa caagggcccg aagtgggaga agagccatgc cgaaattgca
      301 gaacaggcca agcatgaggc cgagatcgag actcggattg ctgagctgcg gaaggagggt
      361 ttctggtcac tgaagaggct gcctaaggtg ccagagcccc ctcgccccaa aggtcactgg
      421 gactatttgt gcgaagagat gcagtggctc tctgctgact ttgctcagga gcgccgttgg
      481 aaacggggtg tggcccggaa ggtggtgcgc atggtgatcc ggcaccacga ggagcagcgg
      541 cagaaagagg aacgggcccg gagggaggag caggccaagc tgcgtcgaat tgcttccacc
      601 atggccaagg atgtcaggca gttctggagc aatgtggaga aggtggtgca attcaagcaa
      661 cagtcccggc ttgaggaaaa gcgcaaaaaa gccctggacc tgcatttgga cttcattgtg
      721 gggcaaactg aaaagtactc ggaccttctg tctcagagcc tcaaccagcc attaacctcc
      781 agcaaagcag gctcttcccc ttgcctcggc tcttcctcag ctgcctccag tcctccaccc
      841 cctgcttctc gcctggatga tgaagatggg gactttcaac cccaagagga tgaggaagag
      901 gatgatgagg aaacgattga agttgaagaa caacaggaag gcaatgatgc agaggcccag
      961 aggcgtgaga ttgagctgct tcgccgtgag ggagaattgc cactggaaga gctgctccgt
     1021 tcccttcccc ctcagctgtt ggaagggcct tccagcccct ctcaaacccc ctcatctcat
     1081 gatagtgaca cccgagatgg gcctgaagaa ggtgctgaag aagagccccc tcaggtgttg
     1141 gagataaagc ccccaccctc tgctgtcaca cagcgcaaca aacagccttg gcatccagat
     1201 gaagatgatg aagagtttac tgccaacgaa gaggaagcgg aggatgaaga ggatactata
     1261 gcagctgagg aacagttgga aggggaggtg gatcatgcca tggagctgag cgagttggct
     1321 cgagaaggtg agctttccat ggaggagcta ttgcagcagt atgcaggagc ctatgcccca
     1381 ggctctggga gcagtgaaga tgaggatgaa gatgaggttg atgctaatag ctctgactgt
     1441 gaaccagagg ggcccgtgga agcggaagag cctcctcagg aggatagtag cagtcagtca
     1501 gactctgtgg aggaccggag tgaggatgag gaagatgaac attcagagga ggaagaaaca
     1561 agtggaagtt cagcatcaga ggaatctgag tctgaagagt ctgaggatgc ccaatcacag
     1621 agccaagcag atgaagagga ggaagatgat gattttgggg tggagtactt gcttgccagg
     1681 gatgaagagc agagtgaggc agatgcaggc agtgggcctc ctactccagg gcccactact
     1741 ctaggtccaa agaaagaaat tactgacatt gctgcagcag ctgaaagtct ccagcccaag
     1801 ggttacacgc tggccacgac ccaggtaaag acgcccattc ccctgcttct gcggggccag
     1861 ctccgggagt accagcacat tgggctagac tggctggtta ccatgtatga gaagaagctt
     1921 aatggcattc ttgctgatga gatggggctt gggaagacca tccagaccat ctctctgctt
     1981 gcccacttgg cttgtgagaa aggtaactgg ggtccccatt taatcattgt tcccaccagc
     2041 gtgatgttga actgggagat ggagttgaaa cggtggtgcc ccagctttaa aatcctcact
     2101 tactatggag cccagaaaga gaggaagctc aagcggcagg gctggaccaa gcccaatgcc
     2161 tttcatgtgt gtatcacatc ttacaagctg gtgctgcagg accaccaggc cttccgtcgc
     2221 aagaactggc gctatctcat tctggatgag gcgcagaaca tcaagaactt caagtcacag
     2281 cgctggcagt cactcctcaa cttcaacagc cagagacgcc tgctcctgac aggaactccc
     2341 ttgcagaaca gcctcatgga gctgtggtcc ttgatgcact ttttgatgcc ccatgtcttc
     2401 cagtctcatc gcgagttcaa ggagtggttc tctaatcccc taactggcat gattgagggc
     2461 agccaagagt ataatgaagg tctagtcaaa cgcctccaca aggttttgag gcctttttta
     2521 ctgcgccgag ttaaggtgga tgttgagaag cagatgccca aaaagtacga gcatgttatc
     2581 cgctgcaggc tctccaagcg tcaacgctgt ctctatgatg acttcatggc acagaccaca
     2641 actaaggaga cactagccac aggccatttc atgagcgtca tcaacatttt gatgcagctg
     2701 agaaaagttt gcaatcatcc aaatctgttc gaccctcgac cggttacctc ccctttcatc
     2761 accccaggca tctgcttcag caccgcctct ctggtgctaa gggccacgga tgtccatccc
     2821 ctccagcgga tagacatggg tcgatttgac cttattggcc tggaaggtcg tgtctctcga
     2881 tatgaggcag acacatttct gccccggcac cgcctctctc gccgggtact gttagaagtg
     2941 gctactgctc ctgacccccc accccggccc aagccagtca agatgaaggt caacaggatg
     3001 ctgcagccag tacctaagca agaaggccgg acagtggtgg tggtgaacaa cccacgggcg
     3061 cccctgggcc ctgtcccagt tcgacctcct ccaggtcctg agctctcagc ccagcccacc
     3121 cctggcccag tcccccaagt gctgccagca tcactgatgg tttcagcctc acctgccggg
     3181 cccccgctta ttcctgcatc tcggcctcct ggccctgtcc tcttgcctcc actgcagccc
     3241 aacagtggtt ctctccccca ggtgttgcca tcccccctgg gggtcctgag tgggacctca
     3301 cggcctccca cgccaacctt gtccctaaag ccaacaccac ctgccccagt tcgcctgagc
     3361 ccagccccac ctccaggctc ctctagcctg ttgaagcccc tgacagtgcc accaggctac
     3421 accttccctc ctgctgctgc caccaccact tctaccacca cggcaactgc taccaccaca
     3481 gcagtgccag ctccgactcc tgcaccacag cgcctcattc tatctcccga tatgcaggct
     3541 cgcctgccct caggcgaagt ggtcagcatc gggcagttag cctcactggc acaacgtcca
     3601 gtggctaatg cagggggaag caaacctctc accttccaaa tccagggcaa caagctgact
     3661 ttgactggtg cccaggtgcg ccagcttgct gtggggcagc cccgcccgct gcaaaggaat
     3721 gtggtgcacc tcgtgtcagc aggggggcag caccatctca tcagccagcc tgcccatgtg
     3781 gccctcatcc aggccgtggc cccgacccct ggccctaccc ctgtctctgt gctgccttct
     3841 tcgaccccca gcaccacccc tgcccctact ggcctcagcc ttccgcttgc tgctaaccag
     3901 gtgccaccaa ccatggtgaa taatacaggc gtggtgaaga ttgtagtgag acaagcccct
     3961 cgggatggac tgactcctgt tcctccattg gccccagcac cccggcctcc gagctctggg
     4021 cttccagctg tgttgaatcc acgccccacg ttaacccctg gccggctacc cacacctact
     4081 ctgggtactg ctcgagcccc catgcccaca cccactctgg tgaggcctct tctcaagctg
     4141 gtccacagtc cttcacctga agtcagtgct tcagcccccg gagctgcccc cttgaccatc
     4201 tcttctcctc tccacgtgcc atcctccctc cctgggccag cctcttctcc aatgccaatt
     4261 cccaactcct ctccccttgc tagtcctgtg tcctctacag tctcagttcc attgtcatct
     4321 tcactcccca tctctgtccc caccacactt cctgccccag cctcggctcc actcaccatc
     4381 cccatctcag cccccttgac tgtttctgct tcgggcccag ctctgttgac cagtgtgact
     4441 ccaccattgg cacctgttgt cccagcggct cctggacctc cctccttggc accatctggt
     4501 gcttccccgt cagcatcagc cttgactcta ggtttggcca cagctccatc cctgtcttca
     4561 tctcagacac ctggtcaccc tctgttgttg gctcccacct cttcacatgt tccagggttg
     4621 aactcaaccg tggccccagc atgctcacct gtcctggtgc cagcttcggc tctggccagt
     4681 ccttttccgt cagcaccaaa tccagctcca gctcaggctt cccttctggc tccagcatct
     4741 tctgcatctc aggctctagc cacccctctg gctcctatgg cggctccaca gacagcaatt
     4801 ctggctcctt ctccagctcc tcctctggct cctcttccgg tcctggcacc atcgccaggt
     4861 gctgctcctg tcctggcttc atcacagact ccggttccag ttatggctcc atcgtctact
     4921 ccaggaacct ctttagcctc agcttcaccg gtaccagctc caacccctgt gttggctcca
     4981 tcatcaactc aaactatgct accagccccg gttccgtcac ctctcccgag cccggcttct
     5041 acgcagacac tggccctagc cccagcttta gcacccactc ttggaggctc atctccatct
     5101 cagacactct ctttgggaac ggggaacccc cagggaccct ttccaactca gacattgtca
     5161 ttaactccag catcatccct ggtaccaact ccagcccaga cactgtcttt ggcaccagga
     5221 ccaccactgg gtccaactca gacgctgtct ctggctccag caccccctct ggctccagct
     5281 tctccagtgg gcccagcccc agctcacacg ctgactttgg ctccagcatc gtcatctgct
     5341 tcactcctgg ccccagcttc agtgcagaca ctgaccttga gccctgcccc agttcctacc
     5401 ctgggcccgg ccgcagctca gaccttggcg ctggccccag cctccacaca gtccccagct
     5461 tcccaggcat cttcccttgt ggtttcggca tctggtgccg ctcccttgcc tgtcaccatg
     5521 gtatcccggc tgcctgtttc caaggatgag cctgacacac tgacattgcg ctctggtccc
     5581 cccagccctc cctccactgc tacctcgttt ggtggccccc ggcctcgacg ccagcccccc
     5641 ccaccacctc gttccccttt ttatctggac tccctggagg aaaagcggaa gcggcagcgg
     5701 tctgaacgcc tggaacggat tttccaactt agtgaggctc atggggccct ggcacctgtg
     5761 tatgggactg aagtcctgga tttctgtacc ctgccccaac ctgttgccag ccccatcggc
     5821 cctcgttctc ctggccccag ccaccccacc ttttggactt ataccgaggc tgcccaccgg
     5881 gctgtactgt ttccccagca gcgactagac cagctgtcag aaatcattga gaggttcatc
     5941 tttgtcatgc ctcctgtgga ggcacctccc ccttccctgc atgcctgcca cccacctcct
     6001 tggctggccc cacgtcaggc agccttccag gagcaattgg cctctgagct ctggccccgg
     6061 gctcgtcctt tgcaccgtat tgtgtgtaac atgcgcaccc agttccctga cttaagactc
     6121 atccagtatg attgcggaaa gttgcagacg ttggcagtgc tgttgcggca gctcaaggca
     6181 gagggccacc gagtgctcat cttcacccag atgacccgaa tgctggatgt attggagcag
     6241 tttctcacct accatggcca tctctacctg cgcctggatg gatctactag agttgaacag
     6301 agacaggcct tgatggaacg gttcaatgca gacaaacgca tattctgctt catcctttca
     6361 actcggagtg ggggtgtggg cgtgaacctg acaggagcag acactgttgt tttttatgac
     6421 agcgactgga atcccaccat ggatgctcag gcccaggacc gctgtcaccg aattggccag
     6481 acccgggatg tccacatata taggcttatc agtgaacgga cagtggagga gaacatccta
     6541 aaaaaggcaa atcagaagag aatgttgggg gacatggcca ttgagggagg caacttcacc
     6601 acagcctatt tcaaacagca gaccatccga gagctgtttg atatgcccct ggaggaacct
     6661 tctagctcat ccgtgccctc tgcccctgaa gaggaggaag agactgtggc cagcaagcag
     6721 actcatattc tggagcaggc attgtgtcgg gcagaagatg aagaggatat ccgtgcagcc
     6781 acccaggcca aggctgaaca ggtggctgag cttgcagaat ttaatgagaa cgatgggttt
     6841 cctgctggtg agggagagga agctggccgg cctggggctg aggatgagga gatgtcccgg
     6901 gctgagcagg aaattgctgc cctcgtagaa cagctgaccc ccattgagcg ctatgccatg
     6961 aaattcctgg aggcctcact ggaggaggtg agccgagagg agctcaaaca ggcagaagag
     7021 caagtggaag ctgcccgcaa agacctggac caagccaagg aggaggtgtt ccgcctaccc
     7081 caagaggagg aggaggggcc gggggctggg gatgagagtt cctgtgggac tggtggaggc
     7141 acccaccggc gcagtaaaaa ggccaaagcc cctgagaggc cggggactcg tgtcagtgag
     7201 cgtcttcgtg gagcccgggc tgagactcaa ggggcaaacc acactcctgt catatccgcc
     7261 catcaaactc gcagcaccac cacaccaccc cgctgcagtc ctgccaggga gcgagttccc
     7321 aggccagcac ctaggcctcg acccactcca gcttcagctc cggctgcaat tcctgccctt
     7381 gttcctgtcc cagtttctgc cccagtaccc atttcagccc caaatccaat aaccattctc
     7441 cctgtccata tcttgccttc tcctccccct ccttcacaga ttcctccttg ttcttctcct
     7501 gcctgcaccc ctcctcctgc ctgtacccct ccaccagctc atacaccgcc tccagcccaa
     7561 acctgtcttg taactccttc ctctcctctc ttgcttggtc caccttctgt gcccatctct
     7621 gcctcagtca ctaatctccc cttgggcttg aggcctgagg cagagctgtg tgcccaggca
     7681 ttggcatctc cagagtccct ggagctggct tctgtggcca gttcagaaac ctcctcactt
     7741 tctcttgtgc cccctaaaga tctgttgcca gttgctgtgg agatcctgcc tgtgtcagag
     7801 aagaaccttt ctctcacccc ttctgcaccc agcctgacct tggaggctgg cagcatcccc
     7861 aatggtcaag agcaggaggc accagattct gctgagggga ccacccttac agtgctgcct
     7921 gaaggtgagg agttgcccct gtgtgtgagt gagagcaatg gcctggagct cccaccctca
     7981 gcagcatctg atgagccact tcaggagcca ctggaggctg acaggacctc ggaagagctg
     8041 acagaggcca agaccccaac ctccagccca gagaagccac aggaactcgt tacagctgag
     8101 gttgcagctc catccacctc atcttcagcc acttcctcgc ctgagggtcc ttcacctgcc
     8161 cgacctcctc ggcgtcgcac cagtgctgat gtggaaatta ggggtcaagg gactggtcgg
     8221 ccaggacaac caccaggccc caaagtgctt cgaaagctgc caggacggct ggtaactgtg
     8281 gtagaggaaa aggaactggt gcggcggcgg cggcagcagc ggggagctgc cagcacccta
     8341 gtgcctgggg tctctgagac tagtgccagc ccgggaagcc cgtctgtccg cagcatgtca
     8401 gggccagaat cctcccctcc cattggtggg ccctgtgaag ctgctccttc atcctcactg
     8461 cccactccac cccagcagcc cttcattgct cgccgtcaca ttgagctggg ggtgactggt
     8521 ggtggcagcc ccgagaatgg agacggagca ctgctcgcca tcaccccacc tgctgtgaaa
     8581 cgtcggaggg ggaggccccc caagaagaac aggtctccag cagatgctgg gagaggtgtg
     8641 gatgaggcac cctcatccac cttgaaggga aaaaccaatg gggctgaccc agtccctggg
     8701 cctgagaccc taattgttgc agatcctgtc ctggaaccac agcttattcc tgggccccag
     8761 cctcttggac cccagccagt tcacagaccc aatcccctcc tgtcacctgt ggagaaaaga
     8821 aggcgaggac gaccccctaa agcacgagat ttgcccatcc ctgggaccat ttcctctgca
     8881 ggggatggca actccgaaag tcggacacag ccacccccac acccatcacc cctaacccca
     8941 ctcccaccac tgctagtttg tcccactgct actgttgcca acactgtcac cactgtcacc
     9001 atttcaacgt ccccacccaa acggaagagg ggccgacctc ccaagaatcc tccatcacct
     9061 cggcccagcc agctccccgt cttggaccgt gacagcactt ctgttctcga gagctgtgga
     9121 ttggggaggc gacggcaacc ccagggccaa ggggagagtg agggtagttc ctctgatgag
     9181 gatggaagcc gccccctcac ccgcctggcc cgccttcggc ttgaagcaga aggaatgcga
     9241 ggacggaaga gtggagggtc catggtggtg gctgtaattc aggatgacct ggacttagca
     9301 gatagcgggc caggcgggtt ggaattgaca ccacctgtgg tctcactaac cccaaaactg
     9361 cgctcgaccc ggctgcgtcc agggtctcta gtccccccac tagagactga gaagttgcct
     9421 cgcaaacgag caggggcccc agttggtggg agtcctgggc tggcaaagcg gggccgccta
     9481 cagcccccaa gtcccctggg gcctgagggt tcagtagagg agtctgaggc tgaagcctca
     9541 ggtgaggagg aggaagggga tgggacccca cgccgacgtc ctggcccccg ccggcttgtt
     9601 gggaccacca accaagggga ccagcgcatc ctgcgcagca gcgcccctcc ctccctggct
     9661 ggccctgctg ttagtcacag aggccgcaag gccaagacgt agtaatgaac tagtccgtaa
     9721 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga taaagtaaac
     9781 aacttggaac taga
//
