LOCUS       sequence-283275-        2453 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0578
FEATURES             Location/Qualifiers
     source          1..2453
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtagccaa acccccagta atgtcttttc attttgccca agacctttgg
       61 ccagagcaga acataaaaga ttctttccag aaagtgacac tgagaagata cggaaaatgt
      121 gaatatgaga atttacagtt aagaaaaggc tgtaaacatg tggatgagtg tacggggcac
      181 aaaggaggtc ataatacagt taaccaatgt ttgacagcta ccccaagcaa aatattccag
      241 tgtaataaat atgtgaaagt ctttgataaa ttttcaaatt caaatagata taagagaaga
      301 catacaggaa acaaacactt caaatgtaaa gaatgtagca aatcattttg cgtgctttca
      361 caactaactc agcatagaag aattcatact agagtgaatt cctacaaatg tgaagaatgt
      421 ggaaaagcct ttaactggtt ctcaactctt actaaacata agagaattca tactggagaa
      481 aagccctaca aatgtgaaga atgtggcaaa gcctttaacc agtcctcaca acttactagg
      541 cataagataa ttcatactga agagaaaccc aacaaatgtg aagaatgtgg caaggccttt
      601 aaacaggcct cacaccttac tatacataaa ataattcata ctggagaaaa accttacaaa
      661 tatgaagaat gtggcaaagt ctttagccag tcctcacacc ttactacaca aaagatactt
      721 cacactggag agaacctcta caagtgtaaa gaatgtggaa aagcttttaa cctattctca
      781 aatcttacta accataagag aattcatgct ggggagaaac cctacaaatg taaagaatgt
      841 ggcagagctt ttaacatatc ctcaaacctt aataaacagg agaaaattca tactggaggg
      901 aaactcaaca aatgtgaaga atgtgacaaa gcttttaacc gatccttaaa acttactgca
      961 cataagaaaa ttctaatgga agagaaaccc tacaaatgtg aagaatgtgg caaagtcttt
     1021 aaccagttct caactcttac tagacataag ataattcata ctggagagaa accctacaaa
     1081 tgtaaagaat gtggcaaagc ttttaaccaa tcttcaaacc ttactgaaca taagaaaatt
     1141 catactgcag agaaatccta taaatgtgaa gaatgtggca aagcttttaa ccaacactca
     1201 aacctaatta accataggaa aatttattct ggagagaaac catacaaatg tgaagaatgt
     1261 ggaaaagctt ttaatcgatc ctcaaccctt actagacata agaaaattca tactggagag
     1321 aaaccctaca aatgtgaaga atgtgacaga gcttttagcc agtcttcaaa ccttactgaa
     1381 cataagaaaa ttcatactgg agagaaaccc tataaatgtg aggaatgtgg caaagctttt
     1441 aaccgattct caacccttac taaacataag agaattcata ctggagaaaa accctataaa
     1501 tgtgaagaat gtggaaaagc ctttaatcaa tcctatcaac ttactagaca taagatagtt
     1561 catactaaag agaaactcaa caaatgtgaa gaatttggca aggcctttaa acagtcctca
     1621 caccgtacta tacataaaat tattcatact ggagagaaac cctacaaatg tgaagaacat
     1681 ggcaaagttt ttaaccagtc ctcaaacctt actacacaaa agataattca tactggagag
     1741 aacctctaca aatttgaaga acatggaaaa gcttttaacc tattctcaaa cattactaac
     1801 cataagataa tttatactgg agagaaaccc cacaaatgtg aagaatgtgg aaaagcttat
     1861 aaccgattct caaacctaac tatacataag agaattcata cgggagagaa accttaccaa
     1921 tgtgcagaat gtggcaaagc ctttaactgc tcctcaaccc ttaatagaca taagataatt
     1981 catactggag agaaacctta caaatgtaaa gaatgtggca aagcttttaa cctatcctca
     2041 acccttactg cacataagaa aattcatact ggagagaaac cctataaatg tgaagaatgt
     2101 ggcaaagctt ttaaccaatc ctcaaacctt actacacata agaaaattca tacttcagag
     2161 aaaccctaca aatgtgaaga atgtggcaaa tcctttaacc agttctcatc tcttaatata
     2221 cataagataa ttcatactgg agagaaaccc tacaaatgtg gagattatgg cagagctttc
     2281 aacctatcct caaatcttac tacacataag aaaattcata ctggagagaa accctacaaa
     2341 tgtgagtatg gcaaaactta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     2401 tggctttata tatcttgtgg aaaggacgat acataacggg ataggagtct gga
//
