LOCUS       sequence-283278-        2564 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0580
FEATURES             Location/Qualifiers
     source          1..2564
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaaccact gacatttaaa gatgtcgcca tagaattctc tctggaggag
       61 tggcaatgcc tggatactgc acagcgggat ttatatagga atgtgttgtt agagaactac
      121 agaaacctgg tctttttggt aatgtctttt cattttgccc aagacctttg gccagagcag
      181 aacataaaag attctttcca gaaagtgaca ctgagaagat acggaaaatg tgaatatgag
      241 aatttacagt taagaaaagg ctgtaaacat gtggatgagt gtacggggca caaaggaggt
      301 cataatacag ttaaccaatg tttgacagct accccaagca aaatattcca gtgtaataaa
      361 tatgtgaaag tctttgataa attttcaaat tcaaatagat ataagagaag acatacagga
      421 aacaaacact tcaaatgtaa agaatgtagc aaatcatttt gcgtgctttc acaactaact
      481 cagcatagaa gaattcatac tagagtgaat tcctacaaat gtgaagaatg tggaaaagcc
      541 tttaactggt tctcaactct tactaaacat aagagaattc atactggaga aaagccctac
      601 aaatgtgaag aatgtggcaa agcctttaac cagtcctcac aacttactag gcataagata
      661 attcatactg aagagaaacc caacaaatgt gaagaatgtg gcaaggcctt taaacaggcc
      721 tcacacctta ctatacataa aataattcat actggagaaa aaccttacaa atatgaagaa
      781 tgtggcaaag tctttagcca gtcctcacac cttactacac aaaagatact tcacactgga
      841 gagaacctct acaagtgtaa agaatgtgga aaagctttta acctattctc aaatcttact
      901 aaccataaga gaattcatgc tggggagaaa ccctacaaat gtaaagaatg tggcagagct
      961 tttaacatat cctcaaacct taataaacag gagaaaattc atactggagg gaaactcaac
     1021 aaatgtgaag aatgtgacaa agcttttaac cgatccttaa aacttactgc acataagaaa
     1081 attctaatgg aagagaaacc ctacaaatgt gaagaatgtg gcaaagtctt taaccagttc
     1141 tcaactctta ctagacataa gataattcat actggagaga aaccctacaa atgtaaagaa
     1201 tgtggcaaag cttttaacca atcttcaaac cttactgaac ataagaaaat tcatactgca
     1261 gagaaatcct ataaatgtga agaatgtggc aaagctttta accaacactc aaacctaatt
     1321 aaccatagga aaatttattc tggagagaaa ccatacaaat gtgaagaatg tggaaaagct
     1381 tttaatcgat cctcaaccct tactagacat aagaaaattc atactggaga gaaaccctac
     1441 aaatgtgaag aatgtgacag agcttttagc cagtcttcaa accttactga acataagaaa
     1501 attcatactg gagagaaacc ctataaatgt gaggaatgtg gcaaagcttt taaccgattc
     1561 tcaaccctta ctaaacataa gagaattcat actggagaaa aaccctataa atgtgaagaa
     1621 tgtggaaaag cctttaatca atcctatcaa cttactagac ataagatagt tcatactaaa
     1681 gagaaactca acaaatgtga agaatttggc aaggccttta aacagtcctc acaccgtact
     1741 atacataaaa ttattcatac tggagagaaa ccctacaaat gtgaagaaca tggcaaagtt
     1801 tttaaccagt cctcaaacct tactacacaa aagataattc atactggaga gaacctctac
     1861 aaatttgaag aacatggaaa agcttttaac ctattctcaa acattactaa ccataagata
     1921 atttatactg gagagaaacc ccacaaatgt gaagaatgtg gaaaagctta taaccgattc
     1981 tcaaacctaa ctatacataa gagaattcat acgggagaga aaccttacca atgtgcagaa
     2041 tgtggcaaag cctttaactg ctcctcaacc cttaatagac ataagataat tcatactgga
     2101 gagaaacctt acaaatgtaa agaatgtggc aaagctttta acctatcctc aacccttact
     2161 gcacataaga aaattcatac tggagagaaa ccctataaat gtgaagaatg tggcaaagct
     2221 tttaaccaat cctcaaacct tactacacat aagaaaattc atacttcaga gaaaccctac
     2281 aaatgtgaag aatgtggcaa atcctttaac cagttctcat ctcttaatat acataagata
     2341 attcatactg gagagaaacc ctacaaatgt ggagattatg gcagagcttt caacctatcc
     2401 tcaaatctta ctacacataa gaaaattcat actggagaga aaccctacaa atgtgagtat
     2461 ggcaaaactt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     2521 atatcttgtg gaaaggacga ctaagtgatg agcattattg cctt
//
