LOCUS       sequence-283281-        1970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0602
FEATURES             Location/Qualifiers
     source          1..1970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggatcgtt gacatttcgg gatgtggcca tacaattctc tctggaggag
       61 tggcaatgcc tggacactgc acagcagaat ttatatagga atgtgatgtt agagaactac
      121 agaaacctgg tcttcctggg tattgctgcc cctaagccag acctgatcat ttttctggag
      181 caaggaaaag agccctggaa tatgaagaga catgagctgg tgaaagaacc gccagttata
      241 tgttctcatt ttgctcaaga cctttggcca gagcagggca gagaagattc tttccaaaaa
      301 gtgatattga gaagatatga aaaatgtgga catgagaatt tacagttaaa aattggttgt
      361 accaatgtgg atgagtgtaa ggtgcacaaa aaaggttata ataagcttaa ccagagtttg
      421 acaactacac aaagcaaagt atttcaatgt ggcaaatatg caaacatctt tcataaatgt
      481 tcaaattcaa aaagacataa gataaggcat actggaaaga aacttttgaa atgtaaagaa
      541 tatgtcagat cattttgcat gctttcacac ctatctcaac ataaaagaat ttatactaga
      601 gagaattcct acaaaagtga agaacatggc aaagccttta actggtcctc agcccttact
      661 tataagagaa ttcatactgg agagaaaccc tgcaaatgtg aagaatgtgg caaagccttt
      721 agtaagttct caatccttac taagcataag gtaattcata ctggagagaa acattacaaa
      781 tgtgaagaat gtggcaaagc cttcacccgg tcctcaagcc ttattgaaca taagagaagt
      841 catgctggag agaaacccta caaatgtgaa gaatgtggca aagcctttag taaggcctca
      901 acccttactg cacataagac aattcatgct ggagagaaac cctacaaatg tgaagaatgt
      961 ggcaaagctt tcaaccggtc ctcaaacctt atggaacata agagaattca tactggagag
     1021 aagccctgca aatgtgaaga atgtggcaaa gcctttggta acttctcaac ccttactaaa
     1081 cataaggtaa ttcatactgg agagaaaccc tacaaatgtg aagaatgtgg caaagccttc
     1141 agctggccct caagccttac tgaacacaag agaattcatg ctggagacaa accttacaaa
     1201 tgtgaagaat gtggcaaaac ctttaagtgg tcctcaacac ttactaaaca taagataatt
     1261 catactggag agaaacccta caaatgtgaa gaatgtggca aagcctttac tacattttcg
     1321 agccttacta aacataaagt aattcatact ggagagaaac actacaaatg tgaagaatgt
     1381 ggcaaagtct tcagctggtc ctcaagcctt actacacata aggcaattca tgctggagag
     1441 aaactctaca aatgtgaaga atgtggcaaa gcctttaagt ggtcctcaaa ccttatggaa
     1501 cataagagaa ttcatactgg agagaaaccc tacaaatgtg aagaatgtgg caaagccttc
     1561 agtaaggttg caaaccttac taaacataag gtaattcata ctggagagaa acaatacaaa
     1621 tgtgaagaat gtggcaaagc cttcatctgg tcctcaagac ttagtgaaca taagagaatt
     1681 catactggag agaaacccta caaatgtgaa gaatgtggca aagcctttag ctgggtctca
     1741 gtccttaaca aacataagaa aattcatgct ggaaagaaat tctacaaatg tgaagaatgt
     1801 ggtaaagact tcaaccaatc ctcacacctt actactcata agagaattca tactggagga
     1861 aaaaccctac aaatgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1921 ctttatatat cttgtggaaa ggacgaccga atcccaagag ttaatgcgcg
//
