LOCUS       sequence-283284-        1736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0614
FEATURES             Location/Qualifiers
     source          1..1736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      349..358
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgcttaaaga gcatccagag atggcggaag ctcctcagca gcagttgggt
       61 attcctgtgg tgaaactgga gaaagagttg ccatggggca gaggaaggga ggaccctagt
      121 ccagagactt ttcggctgag gtttcggcag ttccgctacc aggaggcagc tggaccccag
      181 gaagctctta gggagctcca ggagctctgt cgtcggtggc tgaggcccga gttgcacacc
      241 aaggagcaga tcctggagct gctggtgctg gagcagttcc tcactatcct gccccgcgag
      301 ttctacgcct ggatccggga gcatggccca gagagtggca aggccctggc cgccatggtg
      361 gaggacctga cagaaagagc actggaggcc aaggcggttc catgccacag gcagggagag
      421 caggaggaaa cagcactttg cagaggcgct tgggagccag gcatccagct ggggccagtg
      481 gaggtcaagc ctgaatgggg gatgccccct ggggaaggag ttcaaggtcc agacccaggt
      541 accgaggagc agctcagtca ggaccctgga gatgagacac gggccttcca ggagcaagca
      601 ctacctgttc tgcaggcggg tcctggcctc cccgcagtga atcccagaga ccaagagatg
      661 gcagctgggt tctttactgc tggatcgcag gggttggggc catttaaaga tatggccctg
      721 gccttccctg aggaggagtg gaggcatgtg accccagccc agatagactg ctttggggag
      781 tatgtggaac cgcaggactg cagggtctct ccaggcggtg ggagcaagga aaaggaggca
      841 aaacccccac aggaagacct gaaaggggcg ctggtggcac tgacatcaga gaggtttggg
      901 gaagcctctc tccagggccc tgggctcgga agggtctgtg agcaggagcc tggtggccct
      961 gcaggcagtg cgcctgggct tcctcctccc cagcacggtg ccatccccct gcctgacgaa
     1021 gtcaaaaccc acagctcctt ctggaagcct ttccagtgcc ctgagtgtgg gaaaggattc
     1081 agtcggagct ccaatctcgt caggcaccag cgaacccacg aagagaagtc ttatggctgt
     1141 gtggagtgtg ggaagggctt taccctgaga gaatacctga tgaagcacca gagaacccac
     1201 ctgggaaaga ggccctacgt gtgcagcgag tgctggaaaa ccttcagcca gagacaccac
     1261 ctggaggtgc accagcgcag ccacactggg gagaagccct acaagtgcgg ggactgctgg
     1321 aagagcttca gccgcaggca gcacctgcag gtgcaccgga ggacgcacac cggggagaag
     1381 ccctacacct gcgagtgtgg caagagcttc agcaggaatg ccaatctggc ggtgcaccgg
     1441 cgtgcccaca ctggcgagaa gccatatggg tgccaggtgt gcgggaagcg gttcagcaaa
     1501 ggggagcggc tggtccgaca ccagagaatc catacagggg agaagcccta ccactgtcct
     1561 gcctgcgggc gaagcttcaa ccagaggtcc atcctcaacc ggcaccagaa gacccagcac
     1621 cgccaggagc cgctggtgca gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1681 tcttggcttt atatatcttg tggaaaggac gaagcgtata ggcttaggtc agtgac
//
