LOCUS       sequence-283287-        3233 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0619
FEATURES             Location/Qualifiers
     source          1..3233
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctatggc cctggaattg caagcccagg catctccgca gccagagcct
       61 gaagaactcc tgattgtgaa actggaagag gactcttggg gatcagaatc caaactctgg
      121 gagaaggacc gtggctctgt ctctggccca gaggcctccc gccagcgctt caggcaattc
      181 caatacaggg atgcagctgg accccacgag gccttcagcc agctctgggc tctctgctgt
      241 cgttggctga ggccggagat ccgtctcaaa gagcagatcc tggagctgct cgtgctggag
      301 cagttcctga ctatcttgcc tagggaggtc cagacctggg tgcaggcacg ccaccctgag
      361 agtggtgagg aggctgtggc cttggtggag gattggcacc gagagaccag gactgcagga
      421 cagtcgggac tggaattgca tacagaagag accaggccct taaagacagg ggaagaagct
      481 cagagcttcc agctgcagcc agtggatccc tggcctgagg gacagtccca gaagaagggg
      541 gtgaagaata catgccctga ccttcccaat cacctaaatg ccgaggtggc accacagcct
      601 ttgaaagaga gtgctgtcct cactccccga gtccctactc tcccaaagat ggggagcgtt
      661 ggagattggg aggtgacagc tgagtcccag gaagccctgg gccctggcaa acatgctgag
      721 aaggagctct gtaaagaccc cccaggagac gactgtggga acagcgtgtg cctgggagtt
      781 ccagtttcaa aaccaagtaa tacctccgag aaagagcaag gaccagagtt ttggggtcta
      841 agtcttataa attctgggaa aaggagcact gcagattaca gcctggataa tgagccagct
      901 caggcattga cctggaggga ttcaagagcc tgggaggaac aataccagtg ggatgtggag
      961 gacatgaagg tgtcaggtgt tcactggggc tatgaggaga ccaagacttt cctggcaatt
     1021 ttgagtgaat ctcctttctc tgaaaagctc cggacttgtc accagaaccg ccaggtatat
     1081 cgggccattg cagagcagct aagggcaagg ggcttcctgc ggacactgga gcaatgtcgc
     1141 tatagggtca aaaacctcct acggaattac cggaaagcca agagcagcca cccaccaggt
     1201 acctgcccct tctatgagga gctggaggcc ctggtcaggg ctcggacagc catcagagcc
     1261 acagatggcc caggagaggc cgtggcactt cccaggctcg ggtatagtga cgcagagatg
     1321 gatgagcagg aggaaggggg ctgggatcct gaagaaatgg cagaagactg taacggtgct
     1381 ggcctggtca atgttgagtc tacccagggg cccaggattg caggggcccc agctctgttc
     1441 cagagtcgta ttgcaggtgt gcactggggc tatgaggaga ccaaggcctt cctggcaatt
     1501 ctcagtgagt ccccattctc ggaaaagctt cgtacctgtc accagaacag ccaggtgtac
     1561 cgggccattg cagagcggct gtgtgctctg ggcttcctgc ggacactgga gcagtgtcgc
     1621 tacagattca aaaacctcct tcgaagctac cggaaagcca agagcagcca cccaccaggg
     1681 acatgccctt tctatgagga actggactcg ctgatgaggg ctcgggctgc agtcagggcc
     1741 atggggactg tccgagaggc tgcaggtctc cctaggtgtg ggcagagtag tgctgagact
     1801 gatgcccagg aggcctgggg tgaagtggcc aatgaagatg ctgtcaaacc ttcaaccttg
     1861 tgtcctaaag ccccagacat gggttttgaa atgaggcatg aggatgaaga ccagatttca
     1921 gagcaggaca tttttgaggg tttgcctgga gccttatcaa aatgtcctac agaagctgtt
     1981 tgccaacctc ttgactgggg agaagacagt gaaaatgaaa atgaagatga agggcagtgg
     2041 ggaaatccct cacaggaaca gtggcaagaa agttcttctg aagaggactt agaaaaactt
     2101 attgaccatc aaggcctgta ccttgcagag aaaccctaca agtgtgacac atgcatgaag
     2161 agcttcagtc ggagctccca cttcattgcc catcagcgaa tccacacagg tgagaagccc
     2221 tacaaatgcc ttgaatgtgg aaaaaacttt agtgaccgct ctaacctcaa tacccatcag
     2281 agaatccaca ctggagagaa gccctataaa tgccttgaat gtgggaaaag ctttagtgac
     2341 cattctaatc tcatcactca ccagagaatt cacacggggg aaaagcccta taaatgtgga
     2401 gaatgttgga aaagcttcaa ccagagctca aaccttctga aacatcagag aatccacttg
     2461 ggaggaaatc ctgaccagtg tagtgagcct gggggaaact ttgcccaaag cccatctttt
     2521 agtgctcact ggaggaattc tacagaagag acagctcctg aacaacctca aagtatcagt
     2581 aaggacttga attctcctgg accacacagc acaaactcag gggagaaact ttatgagtgt
     2641 tctgaatgtg gaagaagctt ctctaagagc tctgccctca ttagtcacca aagaatccat
     2701 acgggagaga aaccatatga atgtgccgaa tgtgggaaaa gcttcagtaa gagctccacc
     2761 ctggccaacc accagcgcac ccacactgga gagaagccgt ataaatgtgt ggactgtggg
     2821 aagtgcttca gtgagcgctc caagctcatc acacaccaga gagtgcacac aggagagaag
     2881 ccctacaaat gccttgagtg tggaaaattc ttccgtgacc gttctaacct cattactcac
     2941 cagaggattc atacgggaga gaagccgtat aagtgcagag agtgtgggaa atgctttaac
     3001 cagagctcca gtcttattat tcaccagaga atccacacag gggagaaacc ctacaagtgc
     3061 acagagtgtg gcaaagactt caacaacagt tcccacttca gtgctcaccg gagaacccat
     3121 gcaggaggga aggcgtcgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     3181 tggctttata tatcttgtgg aaaggacgaa agttacgagc tgtctttcca ata
//
