LOCUS       sequence-283293-        3485 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0635
FEATURES             Location/Qualifiers
     source          1..3485
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1826..1837)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accgccacca tggcggaaaa caaaggcggc ggcgaggctg agagcggcgg cgggggcagc
       61 ggcagcgcgc cggtaactgc cggggccgcc gggcccgccg cgcaggaggc ggagccgcct
      121 ctcaccgcgg tgctggtgga ggaggaggag gaggaaggcg gcagggccgg cgctgagggc
      181 ggcgcggccg ggcccgacga cgggggggtg gccgcggcct cctcgggctc ggcccaggct
      241 gcttcatctc ctgcggcctc agtgggcact ggagttgccg ggggcgcagt atcgacgccg
      301 gctccagctc cagcctcggc tcccgctccg ggtccctcgg cagggccgcc tcctggaccg
      361 ccagcctcgc tcctggacac ctgcgccgtg tgtcagcaga gcttgcagag ccggcgtgag
      421 gcggagccca agctgctgcc ctgtcttcac tccttctgcc tgcgctgcct gcccgagccg
      481 gagcgccagc tcagcgtgcc catcccgggg ggcagcaacg gcgacatcca gcaagttggt
      541 gtaatacggt gcccagtatg ccgccaagaa tgcagacaga tagaccttgt ggataattat
      601 tttgtgaaag acacatctga agctcctagc agttctgatg aaaaatcaga acaggtatgt
      661 actagttgtg aagacaatgc aagtgcagtt ggcttttgtg tagaatgtgg agagtggcta
      721 tgtaagacat gtatcgaagc acatcaaaga gtaaaattta ctaaagatca cttgatcagg
      781 aagaaagaag atgtctcaga gtctgttgga gcatctggtc aacgccctgt tttctgccct
      841 gtacacaaac aagaacagtt gaaacttttc tgtgaaacat gtgatagatt gacatgtaga
      901 gactgtcagc tattggaaca caaagaacat aggtatcagt ttttggaaga agcttttcaa
      961 aatcagaagg gtgcaattga gaatctactg gcgaaacttc ttgagaagaa gaattatgtt
     1021 cattttgcag ctactcaggt gcagaatagg ataaaagaag taaatgagac taacaaacga
     1081 gtagaacagg aaattaaagt ggccattttc acccttatca atgaaattaa taagaaagga
     1141 aaatctctct tacaacagct agagaatgtt acaaaggaaa gacagatgaa gttactacag
     1201 cagcagaatg acatcacagg cctttcccgg caggtgaagc atgttatgaa cttcacaaat
     1261 tgggcaattg caagtggcag cagcacagca ctactataca gcaagcgact gattactttc
     1321 cagttgcgtc atattttgaa agcacggtgt gatcctgtcc ctgctgctaa tggagcaata
     1381 cgtttccatt gtgatcccac cttctgggca aagaatgtag tcaatttagg taatctagta
     1441 atagagagta aaccagctcc tggttatact cctaatgttg tagttgggca agttcctcca
     1501 gggacaaacc acattagtaa aacccctgga cagattaact tagcacagct tcgactccag
     1561 cacatgcaac aacaagtata tgcacagaaa catcagcagt tgcaacagat gaggatgcag
     1621 caaccaccag cacctgtacc aactacaaca acaacaacac aacagcatcc tagacaagca
     1681 gcccctcaga tgttacaaca acagcctcct cgattgatca gtgtgcaaac aatgcaaaga
     1741 ggcaacatga actgtggagc ttttcaagcc catcagatga gactggctca gaatgctgcc
     1801 agaataccag ggatacccag gcacagcggc cctcaatatt ccatgatgca gccacacctc
     1861 caaagacaac actcaaaccc agggcatgct ggaccctttc ccgtagtatc ggtacacaac
     1921 accacaatca acccaacgag ccctactaca gcaactatgg caaatgcaaa ccgaggtccc
     1981 accagcccat ctgttacagc aatagagcta atcccctcag ttaccaatcc agaaaacctt
     2041 ccatcgctgc cagatattcc acccatacag ttggaagatg ctggctcaag tagtttagat
     2101 aatctactaa gtagatacat ctcaggcagt cacctacccc cacagcctac aagcaccatg
     2161 aatccttctc caggtccctc tgccctttct ccgggatcat caggtttatc caattctcac
     2221 acacctgtga gacccccaag tacttctagt actggcagtc gaggcagctg tgggtcatca
     2281 ggaagaactg ctgagaagac aagtcttagt ttcaaatctg atcaggtgaa ggtcaagcaa
     2341 gaacctggga ctgaagatga aatatgtagc ttttcaggag gtgtaaaaca agaaaaaaca
     2401 gaggatggca ggaggagtgc ttgcatgttg agcagtcctg agagtagctt gacaccacct
     2461 ctctcaacca acctgcatct agaaagtgaa ttggatgcat tggcaagcct ggaaaaccat
     2521 gtgaaaattg aacctgcaga tatgaatgaa agctgcaaac agtcagggct cagcagcctt
     2581 gttaatggaa agtccccaat tcgaagcctc atgcacaggt cggcaaggat tggaggagat
     2641 ggcaacaata aagatgatga cccaaatgaa gactggtgtg ctgtctgcca aaacggagga
     2701 gatctcttgt gctgcgaaaa atgtccaaag gtctttcatc taacttgtca tgttccaaca
     2761 ctacttagct ttccaagtgg ggactggata tgcacatttt gtagagatat tggaaagcca
     2821 gaagttgaat atgattgtga taatttgcaa catagtaaga aggggaaaac tgcgcagggg
     2881 ttaagccccg tggaccaaag gaaatgtgaa cgtcttctgc tttacctcta ttgccatgaa
     2941 ttaagtattg aattccagga gcctgttcct gcttcgatac caaactacta taaaattata
     3001 aagaaaccaa tggatttatc caccgtgaaa aagaagcttc agaaaaaaca ttcccaacac
     3061 taccaaatcc cggatgactt tgtggccgat gtccgtttga tcttcaagaa ctgtgaaagg
     3121 tttaatgaaa tgatgaaagt tgttcaagtt tatgcagaca cacaagagat taatttgaag
     3181 gctgattcag aagtagctca ggcagggaaa gcagttgcat tgtactttga agataaactc
     3241 acagagatct actcagacag gaccttcgca cctttgccag agtttgagca ggaagaggat
     3301 gatggtgagg taactgagga ctctgatgaa gactttatac agccccgcag aaaacgccta
     3361 aagtcagatg agagaccagt acatataaag tagtaatgaa ctagtccgta acttgaaagt
     3421 atttcgattt cttggcttta tatatcttgt ggaaaggacg acctgaaata cctgctgagg
     3481 aagtc
//
