LOCUS       sequence-283299-        4625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0681
FEATURES             Location/Qualifiers
     source          1..4625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1582..1593)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accgccacca tgccccgccg ccgccccccg gcttccggtg ctgcgcagtt tccggagcgg
       61 atcgcaaccc ggagtccgga tccgatcccg ctctgcacat tccaaaggca gccgcgcgcc
      121 gccccggtcc agccgccatg ccgactgttc tttgttacat tcgccggctg cgggcaccgt
      181 tggcgatcgg agtcaaaacc cggctggatt tcccggagcc gctccgggat cgccctgcgc
      241 gccgcccgcc cgccggggtc ttcgccgccc cggcccgcgg ccccgcggcc cccgccgccg
      301 ggcggcgtcg tcgccgaggc cccaggggat gtcgttatcc cccgccctcg ggtacagccc
      361 atgcgggtcg cacggggggg tccctggacc cccaaccccg cgtttagaga agctgagtcc
      421 tggtcccaga ttgggaacca gagggtcagt gagcagctct tggaaacatc tctagggaat
      481 gaggtgtccg acactgagcc actgagccct gcgagtgcag gcctgcgacg caatcctgcc
      541 ctacctcctg gaccctttgc acaaaacttt tcctggggga accaggaaaa tctgccccca
      601 gccctgggga agattgcgaa cggaggagga actggggcag gcaaggccga atgcggctat
      661 gaaactgagt cacacttgct agagccgcac gagatacctt tgaacgtgaa tacacacaag
      721 ttcagtgact gtgagtttcc atatgagttt tgcacggtct gcttttcacc cttcaagctg
      781 ctggggatga gcggggtgga gggcgtgtgg aatcagcatt caagaagtgc cagcatgcac
      841 actttcctaa accactcagc aacgggcatc agagaagcag gttgcaggaa ggacatgccg
      901 gtgtcagaga tggctgaaga tgggagcgaa gagatcatgt tcatctggtg tgaagactgc
      961 agccagtacc acgactccga atgtcccgag ctgggcccag tggtcatggt caaagactcc
     1021 tttgtgttaa gcagggcaag gtcttggcct gccagcggac acgtgcacac ccaggcgggg
     1081 caggggatgc ggggttatga ggacagggac agggctgacc cacagcagct tccagaagca
     1141 gtccctgcag gcctggtgag gcggctcagt gggcagcagc tgccctgccg ttccaccctc
     1201 acctggggga ggctgtgcca cctggtggcc cagggcaggt catcccttcc tcccaacttg
     1261 gagatcagac gactggaaga tggagccgag ggggtgttcg ccatcactca gctcgtcaag
     1321 cggacacagt tcggtccctt tgagtccagg agggtcgcca aatgggaaaa ggagtctgca
     1381 tttcccctga aggtgttcca gaaggacggg caccccgtgt gcttcgacac ctccaacgag
     1441 gatgactgca actggatgat gctggtgcgg ccagcggcgg aggccgagca ccagaacctg
     1501 acggcctacc agcacggcag cgacgtgtac ttcaccacct ccagagacat ccccccgggt
     1561 accgagctgc gcgtgtggta tgcggccttc tatgccaaga agatggacaa gcccatgctg
     1621 aagcaggccg gctctggcgt ccacgctgca ggcaccccag aaaacagcgc ccccgtggag
     1681 tcggagccca gccagtgggc gtgtaaagtg tgttctgcca ccttcctgga gctgcagctc
     1741 ctcaatgaac atctgttggg ccacttagaa caagccaaaa gccttcctcc aggcagccaa
     1801 agcgaggcag cagctcccga gaaggagcag gacacacccc ggggggaacc ccctgcagtg
     1861 cccgagagcg agaatgttgc caccaaagaa cagaagaaaa agcctcgaag ggggagaaaa
     1921 cccaaagtgt ccaaagctga gcagcctcta gtcatcgtgg aagacaagga acccacagag
     1981 caagtggcag agatcattac cgaggtccct ccggatgagc ctgtgagtgc aacgccagat
     2041 gagcggatca tggagctggt tctggggaag ctggccacca ccaccactga caccagctcg
     2101 gttccaaagt tcacccatca tcagaataac accatcacgc tcaagaggag cttaattctc
     2161 tcaagcagac acggcatccg gcgcaagctc atcaaacagc tcggggagca caagcgggtt
     2221 taccagtgca atatctgcag caagatcttc cagaacagca gcaacctgag caggcacgtg
     2281 cgctcgcatg gtgacaagct gtttaagtgc gaagagtgtg caaaattgtt cagccgcaaa
     2341 gagagcctaa agcagcacgt ttcctacaag cacagcagga acgaggtgga cggcgagtac
     2401 aggtaccgct gcggcacttg tgagaagacc ttccgcatcg agagcgcgct ggagttccac
     2461 aactgcagga cagatgacaa gacgttccaa tgtgagatgt gtttcagatt cttctccacc
     2521 aacagcaacc tctccaagca caagaagaag cacggcgaca agaagtttgc ctgtgaggtc
     2581 tgcagcaaga tgttctaccg caaggacgtc atgctggacc accagcgccg gcacctggaa
     2641 ggagtgcggc gagtgaagcg agaggacctg gaggccggtg gggagaacct ggtccgttac
     2701 aagaaggagc cttccgggtg cccggtgtgt ggcaaggtgt tctcctgccg gagcaatatg
     2761 aacaagcacc tgctcaccca cggcgacaag aagtacacct gcgagatctg cgggcgcaag
     2821 ttcttccgcg tggatgtgct cagggaccac atccatgtcc acttcaagga catcgcgttg
     2881 atggatgacc accagaggga agagtttatc ggcaagatcg ggatctcctc ggaagaaaac
     2941 gatgacaatt ctgacgagag cgcagactcg gagcctcaca agtacagctg caagcggtgc
     3001 cagctcacct tcggccgggg gaaggagtac ctgaagcaca tcatggaggt gcacaaggag
     3061 aagggctatg gctgcagcat ctgcaaccgg cgctttgcac tgaaggccac ctaccacgcc
     3121 cacatggtca tccaccgtga aaacctgccg gaccccaacg tgcagaagta catccacccc
     3181 tgcgagatct gcgggcggat cttcaacagc atcgggaacc tggagcgcca caagctcatc
     3241 cacacaggtg tgaagagcca cgcctgcgag cagtgtggga agtcctttgc caggaaggac
     3301 atgctgaagg agcacatgcg tgtgcacgac aatgtccgcg agtacctgtg tgccgagtgt
     3361 gggaaaggca tgaagaccaa gcacgcgctg cgccaccaca tgaagctgca caagggcatc
     3421 aaggagtacg agtgcaagga gtgccaccgc aggttcgcgc agaaggtcaa catgctcaag
     3481 cactgcaagc ggcacacggg gattaaagat ttcatgtgtg aattgtgtgg gaagacattc
     3541 agcgagagga acaccatgga gacccacaag ctcatccaca cagtgggcaa gcagtggacg
     3601 tgctccgtgt gcgacaagaa gtacgtgacc gagtacatgc tgcagaagca cgttcagctc
     3661 acacacgaca aggtggaggc gcagagctgc cagctgtgcg ggaccaaggt gtccaccagg
     3721 gcctccatga gccgacacat gcggcgcaag caccccgagg tgctcgcggt gaggatcgat
     3781 gacctggacc acctcccgga gaccaccacc atcgacgcct cctccattgg catcgtccag
     3841 cctgagctga ctctggagca ggaggatttg gccgaaggga agcacgggaa agctgccaag
     3901 cgaagtcaca agagaaagca gaagccagaa gaggaggcgg gtgctccggt gcccgaggac
     3961 gccaccttca gcgaatactc agagaaagag acggagttca caggcagtgt aggcgacgag
     4021 accaattccg cagtacagag cattcagcag gtagtggtga ccctgggtga cccaaatgtg
     4081 accacaccat cgagctcagt cggcttaacc aacatcaccg tgacccccat caccactgcg
     4141 gccgcgactc agtttaccaa tctccagccg gtggccgtgg ggcaccttac cacccctgaa
     4201 cgccagttac agctggacaa ctcaatcctg accgtgacct ttgataccgt cagcggctct
     4261 gccatgttgc acaaccgcca aaatgacgtc cagatccacc cccagccgga agcctcgaac
     4321 ccacagtctg tggcccattt catcaacctg acgaccctgg tcaactccat cacgcccctg
     4381 gggagccagc ttagtgacca gcacccgctc acgtggcggg cagtgcccca gactgacgtc
     4441 ttgccaccct cgcagccgca ggcaccccca cagcaggcgg cccagcccca ggtgcaggcg
     4501 gagcagcagc agcagcagat gtacagctac tagtaatgaa ctagtccgta acttgaaagt
     4561 atttcgattt cttggcttta tatatcttgt ggaaaggacg accatgaggt aggccaagtg
     4621 accca
//
