LOCUS       sequence-283302-        3527 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0682
FEATURES             Location/Qualifiers
     source          1..3527
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(484..495)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accgccacca tggctgaaga tgggagcgaa gagatcatgt tcatctggtg tgaagactgc
       61 agccagtacc acgactccga atgtcccgag ctgggcccag tggtcatggt caaagactcc
      121 tttgtgttaa gcagggcaag gtcatccctt cctcccaact tggagatcag acgactggaa
      181 gatggagccg agggggtgtt cgccatcact cagctcgtca agcggacaca gttcggtccc
      241 tttgagtcca ggagggtcgc caaatgggaa aaggagtctg catttcccct gaaggtgttc
      301 cagaaggacg ggcaccccgt gtgcttcgac acctccaacg aggatgactg caactggatg
      361 atgctggtgc ggccagcggc ggaggccgag caccagaacc tgacggccta ccagcacggc
      421 agcgacgtgt acttcaccac ctccagagac atccccccgg gtaccgagct gcgcgtgtgg
      481 tatgcggcct tctatgccaa gaagatggac aagcccatgc tgaagcaggc cggctctggc
      541 gtccacgctg caggcacccc agaaaacagc gcccccgtgg agtcggagcc cagccagtgg
      601 gcgtgtaaag tgtgttctgc caccttcctg gagctgcagc tcctcaatga acatctgttg
      661 ggccacttag aacaagccaa aagccttcct ccaggcagcc aaagcgaggc agcagctccc
      721 gagaaggagc aggacacacc ccggggggaa ccccctgcag tgcccgagag cgagaatgtt
      781 gccaccaaag aacagaagaa aaagcctcga agggggagaa aacccaaagt gtccaaagct
      841 gagcagcctc tagtcatcgt ggaagacaag gaacccacag agcaagtggc agagatcatt
      901 accgaggtcc ctccggatga gcctgtgagt gcaacgccag atgagcggat catggagctg
      961 gttctgggga agctggccac caccaccact gacaccagct cggttccaaa gttcacccat
     1021 catcagaata acaccatcac gctcaagagg agcttaattc tctcaagcag acacggcatc
     1081 cggcgcaagc tcatcaaaca gctcggggag cacaagcggg tttaccagtg caatatctgc
     1141 agcaagatct tccagaacag cagcaacctg agcaggcacg tgcgctcgca tggtgacaag
     1201 ctgtttaagt gcgaagagtg tgcaaaattg ttcagccgca aagagagcct aaagcagcac
     1261 gtttcctaca agcacagcag gaacgaggtg gacggcgagt acaggtaccg ctgcggcact
     1321 tgtgagaaga ccttccgcat cgagagcgcg ctggagttcc acaactgcag gacagatgac
     1381 aagacgttcc aatgtgagat gtgtttcaga ttcttctcca ccaacagcaa cctctccaag
     1441 cacaagaaga agcacggcga caagaagttt gcctgtgagg tctgcagcaa gatgttctac
     1501 cgcaaggacg tcatgctgga ccaccagcgc cggcacctgg aaggagtgcg gcgagtgaag
     1561 cgagaggacc tggaggccgg tggggagaac ctggtccgtt acaagaagga gccttccggg
     1621 tgcccggtgt gtggcaaggt gttctcctgc cggagcaata tgaacaagca cctgctcacc
     1681 cacggcgaca agaagtacac ctgcgagatc tgcgggcgca agttcttccg cgtggatgtg
     1741 ctcagggacc acatccatgt ccacttcaag gacatcgcgt tgatggatga ccaccagagg
     1801 gaagagttta tcggcaagat cgggatctcc tcggaagaaa acgatgacaa ttctgacgag
     1861 agcgcagact cggagcctca caagtacagc tgcaagcggt gccagctcac cttcggccgg
     1921 gggaaggagt acctgaagca catcatggag gtgcacaagg agaagggcta tggctgcagc
     1981 atctgcaacc ggcgctttgc actgaaggcc acctaccacg cccacatggt catccaccgt
     2041 gaaaacctgc cggaccccaa cgtgcagaag tacatccacc cctgcgagat ctgcgggcgg
     2101 atcttcaaca gcatcgggaa cctggagcgc cacaagctca tccacacagg tgtgaagagc
     2161 cacgcctgcg agcagtgtgg gaagtccttt gccaggaagg acatgctgaa ggagcacatg
     2221 cgtgtgcacg acaatgtccg cgagtacctg tgtgccgagt gtgggaaagg catgaagacc
     2281 aagcacgcgc tgcgccacca catgaagctg cacaagggca tcaaggagta cgagtgcaag
     2341 gagtgccacc gcaggttcgc gcagaaggtc aacatgctca agcactgcaa gcggcacacg
     2401 gggattaaag atttcatgtg tgaattgtgt gggaagacat tcagcgagag gaacaccatg
     2461 gagacccaca agctcatcca cacagtgggc aagcagtgga cgtgctccgt gtgcgacaag
     2521 aagtacgtga ccgagtacat gctgcagaag cacgttcagc tcacacacga caaggtggag
     2581 gcgcagagct gccagctgtg cgggaccaag gtgtccacca gggcctccat gagccgacac
     2641 atgcggcgca agcaccccga ggtgctcgcg gtgaggatcg atgacctgga ccacctcccg
     2701 gagaccacca ccatcgacgc ctcctccatt ggcatcgtcc agcctgagct gactctggag
     2761 caggaggatt tggccgaagg gaagcacggg aaagctgcca agcgaagtca caagagaaag
     2821 cagaagccag aagaggaggc gggtgctccg gtgcccgagg acgccacctt cagcgaatac
     2881 tcagagaaag agacggagtt cacaggcagt gtaggcgacg agaccaattc cgcagtacag
     2941 agcattcagc aggtagtggt gaccctgggt gacccaaatg tgaccacacc atcgagctca
     3001 gtcggcttaa ccaacatcac cgtgaccccc atcaccactg cggccgcgac tcagtttacc
     3061 aatctccagc cggtggccgt ggggcacctt accacccctg aacgccagtt acagctggac
     3121 aactcaatcc tgaccgtgac ctttgatacc gtcagcggct ctgccatgtt gcacaaccgc
     3181 caaaatgacg tccagatcca cccccagccg gaagcctcga acccacagtc tgtggcccat
     3241 ttcatcaacc tgacgaccct ggtcaactcc atcacgcccc tggggagcca gcttagtgac
     3301 cagcacccgc tcacgtggcg ggcagtgccc cagactgacg tcttgccacc ctcgcagccg
     3361 caggcacccc cacagcaggc ggcccagccc caggtgcagg cggagcagca gcagcagcag
     3421 atgtacagct actagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     3481 tatatatctt gtggaaagga cgaacgagcc ctgcgatgtc gagtata
//
