LOCUS       sequence-283311-        7424 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 7424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0705
FEATURES             Location/Qualifiers
     source          1..7424
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgccacca tgtcaagttc aggttatcct cccaaccaag gagcattcag cacagaacaa
       61 agtcgttatc ctcctcactc tgtccagtat acatttccca acacccgcca ccagcaggag
      121 ttcgcagtcc ctgattatcg ttcctctcat cttgaagtga gtcaggcatc acagcttttg
      181 cagcaacagc agcagcaaca gcttcgaagg cgaccttcct tgctttcaga atttcaccca
      241 ggttctgaca ggcctcaaga aaggagaact agttatgaac cgtttcatcc aggcccatcc
      301 ccagtggatc atgattcact ggaatcgaag cgaccacgtc tggaacaggt ttctgattct
      361 cattttcagc gtgtcagtgc tgcggttttg cctttagtgc acccgctgcc agaagggctg
      421 agggcttctg cagatgctaa gaaggatcca gcattcggag gcaaacatga agctccatcc
      481 tctccaattt cggggcaacc atgtggagat gatcaaaatg cttcaccttc aaaactctca
      541 aaggaagagt taatacagag tatggatcgt gtagatcgag aaattgcaaa agtagaacag
      601 cagatcctta aactgaaaaa gaaacaacaa cagcttgaag aagaggcagc taaacctcct
      661 gagcctgaga agcccgtgtc ccctcctcct gtggagcaga aacaccgcag tattgtccaa
      721 attatttatg atgagaatcg gaaaaaagca gaagaagctc ataaaatttt tgaaggtctt
      781 ggcccaaaag ttgaactgcc actgtataac cagccatcag ataccaaggt gtaccatgag
      841 aacatcaaga caaaccaggt gatgaggaaa aaactcattt tattttttaa aagaagaaat
      901 catgcaagaa aacaaaggga acaaaaaatc tgccagcgtt atgatcagct catggaggca
      961 tgggagaaaa aagtggacag aatagaaaat aatcctcgga ggaaagctaa agaaagcaaa
     1021 acaagggaat actatgaaaa gcagtttcca gaaattcgaa aacaaagaga acagcaagaa
     1081 agatttcagc gagttgggca gaggggagct ggtctttcag ccaccattgc taggagtgag
     1141 catgagattt ctgaaattat tgatgggctc tctgagcagg agaataatga gaaacaaatg
     1201 cggcagctct ctgtgattcc acctatgatg tttgatgcag aacaaagacg agtcaagttc
     1261 attaacatga atgggcttat ggaggaccct atgaaagtgt ataaagatag gcagtttatg
     1321 aatgtttgga ctgaccatga aaaggagatc tttaaggaca agtttatcca gcatccaaaa
     1381 aactttggac taattgcatc atacttggag aggaagagtg ttcctgattg tgttttgtat
     1441 tactatttaa ccaagaaaaa tgagaattat aaagccctcg tcagaaggaa ttatgggaaa
     1501 cgcagaggca gaaaccagca aattgctcga ccctcgcaag aagaaaaagt agaagaaaaa
     1561 gaagaggata aagcagaaaa aacagaaaaa aaagaagaag aaaagaaaga tgaagaggaa
     1621 aaagatgaaa aagaagactc caaagaaaat accaaggaaa aggacaagat agatggtaca
     1681 gcagaagaaa ctgaggaaag agagcaagcc acaccccggg ggcgaaagac tgccaacagt
     1741 cagggccgcc gtaagggccg gatcaccagg tccatgacaa acgaagctgc agctgccagt
     1801 gctgcagccg cagcggctac tgaagagccc ccaccacctc tgccaccgcc accagaaccc
     1861 atttctacag agcctgtgga gacctctcga tggacagaag aagaaatgga agttgctaaa
     1921 aaaggtctag tagaacatgg tcgtaactgg gcagcaattg ctaaaatggt gggaacgaaa
     1981 agtgaagctc aatgtaaaaa cttctatttt aactataaaa ggcgacacaa tcttgacaac
     2041 ctcttacagc agcataaaca gaaaacttca cgaaaacctc gtgaagagcg agatgtgtct
     2101 caatgtgaaa gtgtcgcttc cactgtttct gctcaggagg atgaagatat tgaagcctcc
     2161 aatgaagaag aaaatccaga agacagcgaa gttgaagctg tcaagcccag cgaggacagt
     2221 cctgaaaatg ctacttctcg aggaaacaca gaacctgcgg ttgagcttga gcccaccacg
     2281 gaaactgcac ccagtacatc tccctcctta gcagttccaa gtacaaaacc agctgaagat
     2341 gaaagtgtgg agacccaggt gaatgacagc atcagtgctg agacagcaga gcagatggat
     2401 gtagatcagc aggagcacag tgctgaagag ggttctgttt gtgatccccc acccgctacc
     2461 aaagctgact ctgtggacgt tgaagtgagg gtgccagaaa accatgcatc taaagttgaa
     2521 ggtgataata ccaaagaaag agacttggat agagccagtg agaaggtgga acctagagat
     2581 gaagatttgg tggtagctca gcaaataaat gcccaaaggc ccgagcccca gtcagacaat
     2641 gattccagtg ccacgtgcag cgctgatgag gatgtggatg gagagccaga gaggcagaga
     2701 atgtttccta tggactcaaa gccttcactg ttaaacccca ctggatctat actcgtctca
     2761 tctccgttaa aaccaaatcc actggatctg ccacagcttc agcatcgagc tgctgttatc
     2821 ccaccaatgg tatcctgcac cccatgtaac ataccaattg gaaccccagt gagcggctat
     2881 gctctctacc agcgacacat taaagcaatg catgagtcag cactcctgga ggagcagcgg
     2941 cagagacaag aacagataga tttggaatgt agaagttcta caagtccatg tggcacatcc
     3001 aagagtccaa acagagagtg ggaagtcctt cagcctgctc cacatcaagt gataactaat
     3061 ctccctgaag gcgttcggct tccgacaact cgaccaacca ggccaccgcc ccctctcatc
     3121 ccgtcatcca aaaccacagt ggcttcagaa aaaccatctt ttataatggg aggctccatc
     3181 tcacagggaa caccaggcac ttatttgact tctcataatc aggcttccta cactcaagaa
     3241 acacccaagc cgtcagtggg atctatctct cttggactgc cacggcaaca ggaatctgcc
     3301 aaatcagcta ctttgcccta catcaagcag gaagaatttt ctccccgaag ccaaaactca
     3361 caacctgagg gtctgttggt cagggcccaa catgaaggtg tagtcagagg taccgcagga
     3421 gccatacaag aaggaagtat aactcgggga actccaacca gcaaaatttc agtggagagc
     3481 attccatccc tacggggctc tatcactcag ggcaccccgg ctctgcccca gactggcata
     3541 ccaacagagg ctttggtgaa ggggtccatt tcgagaatgc ccattgaaga cagcagtcct
     3601 gagaaaggca gagaggaagc tgcatccaaa ggccatgtta tttatgaagg caaaagtgga
     3661 catatcttgt catatgataa tattaagaat gcccgagaag ggactaggag tccaagaaca
     3721 gctcatgaaa tcagtttaaa gagaagctat gaatcagtgg aaggaaatat aaagcaaggg
     3781 atgtcaatga gggagtctcc tgtatcagca ccgttagagg ggctgatatg ccgagcatta
     3841 cccaggggga gtcctcattc tgacctcaaa gaaaggactg tattgtctgg ctccataatg
     3901 caggggacac caagagcaac aactgaaagc tttgaagatg gccttaaata tcccaaacaa
     3961 attaaaaggg aaagtcctcc catacgagca tttgaaggtg ccattaccaa aggaaaacca
     4021 tatgatggca tcaccaccat caaagaaatg gggcgttcca ttcatgagat tccaaggcaa
     4081 gatattttaa ctcaggaaag tcggaaaact ccagaagtgg tccagagcac acggccgata
     4141 attgagggtt ccatttccca gggcacacca ataaagtttg acaacaactc aggtcaatct
     4201 gccatcaaac acaatgtcaa atccttaatc acggggccta gcaaactatc ccgtggaatg
     4261 cctccgctgg aaattgtgcc agagaacata aaagtggtag aacggggaaa atatgaggat
     4321 gtgaaagcag gcgagaccgt gcgttcccgg cacacgtcag tggtaagctc tggcccctcc
     4381 gttcttaggt ccacactgca tgaagctccc aaagcacaac tgagccctgg gatttatgat
     4441 gacaccagtg cacggaggac ccctgtgagt tatcaaaaca ccatgtccag aggctcaccc
     4501 atgatgaaca gaacttctga tgttacaatt tcttctaaca agtctaccaa tcatgaaagg
     4561 aaatcgacac tgacccctac ccagagggaa agtatcccag cgaagtctcc agtgcctggg
     4621 gtggaccctg tcgtgagcca cagtccgttt gatccccatc acagaggcag cactgcaggc
     4681 gaggtttatc ggagccacct gcccacgcac ttggatccag ccatgccttt tcacagggct
     4741 ttggatcctg cagcggctgc ttacctgttt cagagacagc tttcaccaac tccaggttac
     4801 ccaagtcagt atcagcttta cgcaatggag aacacaagac agacaatctt aaatgattac
     4861 attacctcac aacagatgca agtgaacttg cgtccagatg tggccagagg actctcccca
     4921 agagagcagc cactgggtct cccataccca gcaacgagag gaatcattga cctgaccaat
     4981 atgcctccaa caattttagt gcctcatcca gggggaacaa gcactcctcc catggacaga
     5041 atcacttata ttcctggtac acagattact ttccctccca ggccgtacaa ctctgcttcc
     5101 atgtctccag gacacccaac acaccttgca gctgctgcaa gtgctgagag ggaacgggaa
     5161 cgggagcggg agaaggagcg ggagcgggaa cggattgctg cagcttcctc cgacctctac
     5221 ctgcggccag gctcagaaca gcctggccga cctggcagtc atggatatgt tcgctcccct
     5281 tccccttcag taagaactca ggagaccatg ttgcaacaga gacccagtgt tttccaagga
     5341 accaatggaa ccagtgtaat cacacctttg gatccaactg ctcagctacg aatcatgcca
     5401 ctgcctgctg ggggcccttc aataagccaa ggcctgccag cctcccgtta caacactgct
     5461 gcggatgccc tggctgctct tgtggatgct gcagcttctg caccccagat ggatgtgtcc
     5521 aaaacaaaag agagtaagca tgaagctgcc aggttagaag aaaatttgag aagcaggtca
     5581 gcagcagtta gtgaacagca gcagctagag cagaaaaccc tggaggtgga gaagagatct
     5641 gttcagtgtt tatacacttc ttcagccttt ccaagtggca agccccagcc tcattcttca
     5701 gtagtttatt ctgaggctgg gaaagataaa gggcctcctc caaaatccag atatgaggaa
     5761 gagctaagga ccagagggaa gactaccatt actgcagcta acttcataga cgtgatcatc
     5821 acccggcaaa ttgcctcgga caaggatgcg agggaacgtg gctctcaaag ttcagactct
     5881 tctagtagct tatcttctca caggtatgaa acacctagcg atgctattga ggtgataagt
     5941 cctgccagct cacctgcgcc accccaggag aaactgcaga cctatcagcc agaggttgtt
     6001 aaggcaaatc aagcggaaaa tgatcctacc agacaatatg aaggaccatt acatcactat
     6061 cgaccacagc aggaatcacc atctccccaa caacagctgc ccccttcttc acaggcagag
     6121 ggaatggggc aagtgcccag gacccatcgg ctgatcacac ttgctgatca catctgtcaa
     6181 attatcacac aagattttgc tagaaatcaa gtttcctcgc agactcccca gcagcctcct
     6241 acttctacat tccagaactc accttctgct ttggtatcta cacctgtgag gactaaaaca
     6301 tcaaaccgtt acagcccaga atcccaggct cagtctgtcc atcatcaaag accaggttca
     6361 agggtctctc cagaaaatct tgtggacaaa tccaggggaa gtaggcctgg aaaatcccca
     6421 gagaggagtc acgtctcttc ggagccctac gagcccatct ccccacccca ggttccggtt
     6481 gtgcatgaga aacaggacag cttgctgctc ttgtctcaga ggggcgcaga gcctgcagag
     6541 cagaggaatg atgcccgctc accagggagt ataagctact tgccttcatt cttcaccaag
     6601 cttgaaaata catcacccat ggttaaatca aagaagcagg agatttttcg taagttgaac
     6661 tcctctggtg gaggtgactc tgatatggca gctgctcagc caggaactga gatctttaat
     6721 ctgccagcag ttactacgtc aggctcagtt agctctagag gccattcttt tgctgatcct
     6781 gccagtaatc ttgggctgga agacattatc aggaaggctc tcatgggaag ctttgatgac
     6841 aaagttgagg atcatggagt tgtcatgtcc cagcctatgg gagtagtgcc tggtactgcc
     6901 aacacctcag ttgtgaccag tggtgagaca cgaagagagg aaggggaccc atcacctcat
     6961 tcaggaggag tttgcaaacc aaagctgatc agcaagtcaa acagcaggaa atctaagtct
     7021 cctatacctg ggcaaggcta cttaggaacg gaacggccct cttcagtctc ctctgtacat
     7081 tcagaagggg attaccatag gcagacgcca gggtgggcct gggaagacag gccctcttca
     7141 acaggctcaa ctcagtttcc ttataaccct ctgactatgc ggatgctcag cagtactcca
     7201 ccaacaccga ttgcatgtgc tccctctgcg gtgaaccaag cagctcctca ccaacagaac
     7261 aggatctggg agcgagagcc tgccccactg ctctcagcac agtacgagac cctgtcggat
     7321 agtgatgact agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     7381 atatcttgtg gaaaggacga tgcacggtgg ccaataacgg agac
//
