LOCUS       sequence-283317-        8258 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8258)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 8258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0751
FEATURES             Location/Qualifiers
     source          1..8258
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    6559..6570
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 accgccacca tgcctcgaac taaacaaatt catcccagaa atctaagaga caaaattgaa
       61 gaagcacaaa aagaacttaa tggggcagaa gtttcaaaaa aagaaatctt acaggctggt
      121 gttaaaggaa cttcggaatc ccttaaaggt gtgaaacgca aaaagatcgt agctgagaat
      181 cacctgaaaa aaataccaaa atccccactg agaaatcctc ttcaggcaaa acataaacaa
      241 aatacagaag agtcatcttt cgccgttctt catagtgctt cggagtctca caagaaacag
      301 aattatattc ctgtaaaaaa tgggaagcag tttaccaaac aaaatggaga aacacctgga
      361 ataattgctg aagcctcaaa atctgaagaa tctgtctccc caaagaagcc cttgtttctg
      421 cagcaaccat ctgaactgcg tagatggaga tccgaaggcg ctgatcctgc caaattcagt
      481 gacctcgatg aacaatgtga ctcaagttcc ttgtcaagta aaaccaggac tgacaatagc
      541 gaatgcatct cttctcattg tggcactacg tccccctcct atacaaacac tgcattcgat
      601 gtcttactga aagcaatgga gccagaactg agcaccttgt cacaaaaggg ctcaccttgt
      661 gcaattaaga cagaaaaact gaggccaaat aaaactgcac gttcccctcc caaattaaaa
      721 aacagttcaa tggatgcccc aaatcagact tcacaggaat tggttgctga atcacagtct
      781 tcttgtacct catacacagt ccatatgtct gctgctcaga agaatgagca aggggcaatg
      841 cagtcagctt ctcatttgta tcatcaacat gaacactttg ttcccaaatc caaccaacat
      901 aatcaacagc ttccggggtg ttcaggtttc acaggatcac tgacaaatct gcaaaatcaa
      961 gagaatgcca aacttgaaca ggtttataat atagcagtga catcatctgt aggcctaact
     1021 tcaccttcca gtagatctca ggttactcct caaaaccagc aaatggattc tgcttcacct
     1081 ttgtcaataa gtccggctaa ttctacacag tcgcccccca tgccaatcta taattcaact
     1141 catgttgcct ctgttgttaa tcaaagcgta gagcaaatgt gcaatcttct tctgaaagat
     1201 cagaagccaa aaaaacaagg aaaatatatt tgtgagtatt gcaatagagc atgtgcaaag
     1261 cctagtgtgc ttttaaagca tatccgctcc cacactggag agcgacccta tccctgtgtg
     1321 acttgtggat tttcatttaa gactaaaagt aatctgtata agcacaagaa atcccacgca
     1381 catactatca aactgggtct tgtcttgcaa ccagatgctg gtggcttgtt cttgtcccac
     1441 gagtccccca aagcacttag tattcattca gacgtagaag acagtgggga gagcgaggag
     1501 gaaggcgcca ctgatgagag acagcatgac ctgggcgcca tggagctgca gcctgtgcac
     1561 ataataaaga ggatgtcaaa tgctgaaact ttactaaaat caagcttcac tccaagcagt
     1621 ccagaaaatg tgataggtga ctttttgcta caggacagat ctgcagaatc acaagctgtg
     1681 acagagttac cgaaagttgt ggtccaccat gtcactgtgt cccccttaag aactgacagt
     1741 ccaaaggcca tggatcccaa gcctgaactt tctagtgcac aaaagcagaa ggaccttcag
     1801 gtgacaaacg tacagccact ttcagccaac atgtcccagg gtggagtctc caggttggag
     1861 actaatgaga attcccacca gaaaggcgac atgaatccac tggaaggaaa gcaagactct
     1921 cacgtaggaa cggtacacgc ccagctacaa aggcagcagg ctaccgatta ctcccaagag
     1981 cagcaaggaa agctcctgag tcctcgaagt ttaggaagta cggattctgg ttacttttca
     2041 cgttctgaaa gtgccgatca aacagtgagt ccaccaactc cctttgccag aaggttaccc
     2101 agcacagaac aagactctgg aaggagtaac ggaccctctg cagctcttgt caccacgtca
     2161 acaccctctg ctttgcccac aggggaaaag gcattgcttt taccaggtca gatgcgccca
     2221 cctttggcca caaaaacact tgaggagcgg atatcgaagc ttatctcaga caatgaagct
     2281 ttggtagatg acaagcaact ggatagtgtg aagccgcgga gaacctcact gtcaagacga
     2341 ggaagcattg attcccccaa atcatacata tttaaagatt ctttccagtt tgatttaaaa
     2401 ccagtgggac ggagaacaag ttcaagctct gatataccga agtcaccttt cacccctact
     2461 gaaaaatcaa agcaagtgtt tcttctgtct gtaccttcac ttgactgttt acctatcaca
     2521 agaagtaatt ccatgccgac cacaggttat tcagcagtac ctgcaaatat aatacctcct
     2581 cctcatccac taagaggaag tcagtcattt gatgacaaaa ttggcgcttt ctatgatgat
     2641 gtctttgtat cgggacctaa cgctcctgtg ccccagagtg ggcatccccg tacacttgtg
     2701 agacaagcag ccatagaaga ctcttcagca aatgaaagtc atgttcttgg tactggacag
     2761 tccctggatg agagccacca aggatgccat gctgctggtg aagccatgtc agtgaggagc
     2821 aaggcactgg cacaaggccc acatatagaa aaaaagaagt ctcatcaagg gcgagggaca
     2881 atgtttgagt gtgaaacttg tagaaacagg tataggaaac tggaaaattt tgaaaatcat
     2941 aagaaatttt actgttctga gttacatgga ccaaaaacaa aggtagccat gagagaacct
     3001 gagcacagcc ctgtgcccgg cggtctgcag cctcagattc tacactacag agtcgctggg
     3061 tcctccggca tctgggaaca gacgccccag ataagaaaaa ggaggaaaat gaaaagtgtt
     3121 ggggatgatg aagaacttca gcaaaatgaa agtggaacat ctccaaaaag ttctgaaggc
     3181 cttcagtttc agaatgctct gggctgtaat cccagtttgc ctaaacataa tgttaccata
     3241 agaagtgacc agcagcataa aaatatacag ttgcaaaact cccatattca ccttgttgcc
     3301 aggggccctg agcagaccat ggatcccaag ctgtcgacca tcatggaaca acagataagt
     3361 tcagcagccc aggacaagat agaactgcag agacacggaa ctggaatctc tgtcatccag
     3421 cacaccaact ccctgagcag gcccaactca tttgacaagc ctgagccttt tgaaagagcc
     3481 tccccagttt ctttccagga gctgaataga acggggaagt ccgggtctct aaaagtgata
     3541 ggaatctccc aagaggaaag tcacccttct cgggacgggt ctcatcctca ccagcttgca
     3601 ctatcagacg ctctcagagg agaacttcag gaaagctcca gaaagagtcc aagtgaacga
     3661 catgtgttag gacagccctc aagacttgtc cggcagcaca acatccaagt tccagagatt
     3721 ttggtcacag aagaaccaga tcgagacctg gaagctcaat gccatgatca agaaaagtca
     3781 gagaagttca gttggcccca gcgtagtgaa accttgtcaa aattgccaac agagaaactg
     3841 ccacccaaaa agaaaaggct ccgtctggct gagatagaac attcctcaac agaatcgagc
     3901 tttgattcca ctctctccag gagtctaagt agggagagca gtttatctca cacttcaagt
     3961 ttctcagcct ctttagacat agaggacgtt tctaaaacgg aggcttcccc caaaatcgat
     4021 tttctaaata aagccgagtt tcttatgatt ccagctggct tgaatactct gaatgttcct
     4081 ggatgtcacc gggaaatgag gcgtactgca tcagaacaga ttaattgcac gcaaacgtca
     4141 atggaggtct ctgatctcag aagcaaatca ttcgattgtg gaagcatcac cccaccccag
     4201 acaacaccac ttactgaatt gcagcctcca tcttcacctt ctcgagtggg agtgactggg
     4261 catgtgcctc tcttagaaag aaggagaggc ccactggtac ggcaaatatc tttaaacata
     4321 gccccagata gtcatctgtc tcctgtacac ccaacatctt tccaaaatac tgctcttccc
     4381 agtgtgaatg cagtgccata tcaggggcct cagctcacta gtacatcttt agctgagttt
     4441 tctgcaaata ctttgcactc tcagactcag gttaaggatc tgcaggcaga aacatcaaac
     4501 tccagctcta ccaacgtttt tcctgttcaa cagctctgtg atatcaattt gttaaatcaa
     4561 atccatgcac cgcctagcca ccagagcaca cagctatctc tgcaagtgtc tacgcagggt
     4621 agcaagccag ataaaaattc tgttttatct gggtcttcta aaagtgagga ttgctttgct
     4681 cccaaatacc aattgcattg tcaggttttc acttcaggcc catcttgctc ttctaatcct
     4741 gtgcattctt tgccaaatca agttatttca gatccagttg gaacagatca ttgtgtgaca
     4801 tcagcaacat taccaaccaa attaattgac agcatgtcta attcgcatcc tctgctacca
     4861 ccagagctca ggccccttgg aagtcaggtg cagaaggtgc catcatcatt catgctgccc
     4921 atacgcctgc agagtagtgt tcctgcttac tgttttgcta cactcacatc cctgccacaa
     4981 atactagtga cccaagatct gcccaatcag ccaatttgcc agactaatca tagtgtagtg
     5041 ccaatcagtg aagaacaaaa ttctgtgcca acattacaaa aaggtcatca gaatgctttg
     5101 ccaaacccag agaaggaatt tctatgtgaa aatgtttttt cagagatgag ccaaaattct
     5161 tctctatcag aatccttgcc cataactcag aaaatatctg ttggtcgact ttcccctcaa
     5221 caagaatctt cagcttcgag taaaaggatg ctttccccag caaatagttt agacattgcc
     5281 atggaaaagc accagaagcg ggccaaagat gaaaatggag ctgtttgtgc aacagacgtg
     5341 agacctttag aggctttgag ttcgagagtt aatgaagcta gtaaacagaa gaagcctatt
     5401 ttagtgagac aggtttgtac tacagagccc ctggacggtg tgatgttgga aaaggatgtt
     5461 ttttctcaac ctgaaattag taatgaggct gttaatttga caaatgtttt accagctgat
     5521 aattcatcaa caggatgctc taaatttgtc gttatagaac ctataagtga attgcaggaa
     5581 tttgaaaaca tcaagtcatc cacatcatta actcttacag ttcgaagttc acctgctcct
     5641 tcagaaaata ctcatatttc tcctttgaaa tgtacagaca ataaccaaga aaggaagtct
     5701 ccaggggtta aaaatcaagg tgacaaagtg aacatccaag agcaaagtca acagccagtc
     5761 acttctcttt cattgtttaa catcaaggac acccagcagc tggctttccc tagcctgaaa
     5821 actacaacca actttacatg gtgttatctc ttaaggcaga agtcgttgca tttgcctcag
     5881 aaggaccaga aaacttcagc ctatactgat tggacagtaa gcgccagtaa tccaaatcca
     5941 ctcggtttgc ccacaaaagt tgcacttgct ctccttaatt caaaacagaa cactggaaaa
     6001 tcactatact gtcaagcaat aactacccat tccaagtcag acttattggt ctattcaagc
     6061 aagtggaaaa gcagcttaag caagagagca ttaggtaatc aaaagtccac agtagttgaa
     6121 ttcagcaata aagatgcctc tgaaattaac agtgagcaag ataaagaaaa ttccttaatc
     6181 aaaagtgaac caagaagaat taaaatattt gatggaggat ataagtcaaa tgaagagtat
     6241 gtatatgtcc gaggcagggg aagaggaaaa tacatttgtg aagaatgtgg aatacgttgt
     6301 aagaaaccta gcatgttaaa gaaacacata cgaacccata cagatgtccg cccctaccac
     6361 tgcacttact gtaacttctc ctttaagact aaaggaaatc tgacaaaaca catgaagtcc
     6421 aaggcacata gcaagaaatg tgtggattta ggcgtctcag taggtttaat agatgaacag
     6481 gatacagaag aatcagatga aaaacagaga ttcagttatg agcgatctgg atatgatctt
     6541 gaagaatctg atggcccaga tgaggatgac aatgaaaatg aagacgatga tgaggacagc
     6601 caggctgaat cagtcctgtc agccacaccc tcagtcacag ctagcccgca gcaccttcca
     6661 tctagaagta gccttcagga ccctgtgagt actgacgagg atgtcaggat caccgattgc
     6721 ttttctgggg tacacacgga cccaatggac gttctgccca gggcgctgct caccagaatg
     6781 actgtcctga gcacagcaca gtctgactac aataggaaga cactctctcc ggggaaggcc
     6841 aggcagcgtg ctgcgagaga tgaaaacgac acaattccgt ctgtagacac ttccaggtcc
     6901 ccgtgtcatc agatgtctgt ggactaccct gagtcagaag aaattctgag aagttctatg
     6961 gcaggaaaag ctgttgctat aacacagagc ccatcatctg taagacttcc tcctgctgca
     7021 gctgagcaca gcccccagac agcagcgggg atgccttctg tggcctcacc acatcctgac
     7081 cctcaagaac agaagcagca aataactcta cagccgactc caggcttgcc ttctccccac
     7141 actcatttgt ttagccacct tcctttgcat tcccagcagc aatcgaggac accttataat
     7201 atggttccag ttggggggat ccatgtggta cctgctggcc tcacatactc cacgtttgtg
     7261 ccccttcagg ctggaccagt gcagctcacg atccctgctg tcagtgtcgt tcacagaact
     7321 ttgggtactc ataggaatac ggtcacagaa gtgtctggca ctacaaaccc tgctggagtg
     7381 gctgaattaa gcagtgttgt gccatgtatt cctatcggcc aaatccgcgt gccaggcctt
     7441 cagaacctaa gtaccccagg cttgcagtca ctcccctcgt taagcatgga aaccgtcaat
     7501 attgtaggcc tagccaatac aaatatggcc ccacaagtcc atccaccagg actggctctg
     7561 aatgctgtcg gactgcaggt tctgactgca aacccttcat cacaaagcag ccccgcccct
     7621 caggcacaca ttccaggtct ccagatcttg aacatagcat tgcccacctt aatcccctca
     7681 gtcagtcaag tagccgttga tgcacaggga gctccagaaa tgccagcttc ccaaagcaaa
     7741 gcatgcgaga cacaacccaa gcagacttct gtagccagcg caaaccaggt cagcaggacc
     7801 gagtctcctc aggggttacc tacagtccag cgggaaaatg caaaaaaagt tctgaatcca
     7861 cctgcccctg caggtgacca tgcaaggctt gatggcctga gtaaaatgga cacagagaag
     7921 gctgcctcgg caaatcacgt gaagcccaag cctgaactca cttccataca gggccaacca
     7981 gcgtccacgt cacaacctct gctgaaggca cattctgaag tttttacaaa gccctcaggc
     8041 cagcagactc tctctccaga cagacaggtt cccaggccca cagcactacc gcggaggcag
     8101 cccactgtgc acttcagcga cgtgagcagc gatgatgacg aggacaggct tgtgatagca
     8161 acctagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     8221 tgtggaaagg acgatctgca tacattacgg tgttgtac
//
