LOCUS       sequence-283320-        1964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0781
FEATURES             Location/Qualifiers
     source          1..1964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggaccatt gcaatttaga gatgtggcca tagaattctc tctggaggag
       61 tggcattgcc tggacactgc acagcggaat ctatatagga atgtgatgtt agagaactac
      121 agtaacctgg tcttccttgg tattgttgtc tctaagccag acctgatcgc ccatctggag
      181 caaggaaaaa aacctttgac tatgaagaga catgagatgg tagccaaccc ctcagttata
      241 tgttctcatt ttgcccaaga tctttggcca gagcagaaca taaaagattc tttccaaaaa
      301 gtgatactga gaagatatga aaaacgtgga catggaaatt tacagttaat aaaaaggtgt
      361 gaaagtgtag atgagtgtaa ggtgcacaca ggaggttata atggacttaa ccagtgtagt
      421 acaactaccc agagcaaagt atttcaatgt gataaatatg ggaaagtctt tcataaattt
      481 tcaaattcaa atagacataa tataagacat actgaaaaaa aacctttcaa atgcatagaa
      541 tgtggcaaag cttttaacca gttctcaacc cttataacac ataagaaaat tcatactgga
      601 gagaaaccct acatttgtga agaatgtggc aaagccttta agtactcctc tgcccttaat
      661 acacataaga gaattcatac tggagagaaa ccatacaagt gtgataaatg tgacaaagcc
      721 tttattgcat cctcaaccct tagtaaacat gagatcattc atactggaaa gaaaccctac
      781 aagtgtgaag aatgtggcaa agcttttaac caatcctcga cacttactaa acataagaaa
      841 attcatactg gagagaaacc ctacaaatgt gaagaatgtg gcaaagcttt taaccaatcc
      901 tcaacactta ctaaacataa gaaaattcat actggagaga agccctacgt ttgtgaagaa
      961 tgtggcaaag cctttaagta ctcccgtatc cttactacac ataagagaat tcatactgga
     1021 gagaaaccat acaagtgtaa taaatgtggc aaagccttta ttgcatcctc aacccttagt
     1081 agacatgagt tcattcatat gggaaagaaa cattacaaat gtgaagaatg tggcaaagcc
     1141 ttcatttggt cctcagtcct aactagacat aagagagttc atactggaga gaagccctac
     1201 aaatgtgaag aatgtggcaa agcctttaag tactcctcta cccttagttc acataagaga
     1261 agtcatactg gagagaaacc ctacaaatgt gaagaatgtg gcaaagcctt tgttgcatcc
     1321 tcaaccctta gtaaacatga gatcattcat actggaaaga aaccctacaa gtgtgaagaa
     1381 tgtggcaaag cttttaacca gtcctcatcc cttactaaac ataagaaaat tcatactgga
     1441 gagaaaccct acaaatgtga agaatgtggc aaagctttta accagtcctc ttcccttact
     1501 aaacataaga aaattcatac tggagagaaa ccctacaaat gtgaagaatg tggcaaagct
     1561 tttaaccagt cctcaaccct tattaaacat aagaaaattc atactagaga gaaaccctac
     1621 aaatgtgaag aatgtggcaa agcttttcac ctatccacac accttactac acataagata
     1681 cttcatactg gagagaaacc ttatagatgt agagaatgtg gcaaagcttt taaccattct
     1741 gcaacccttt cttcacataa gaaaatccat tctggagaga aaccatacga gtgtgataaa
     1801 tgtggcaaag cctttatttc accctcaagc cttagtagac atgagataat tcatactggg
     1861 gagaaaccct agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1921 atatcttgtg gaaaggacga tcatgatgcg gtcagagcaa gccg
//
