LOCUS       sequence-283326-        3098 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0863
FEATURES             Location/Qualifiers
     source          1..3098
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccaagt tgcaatgtcc accctccccg ttgaagatga ggagtcctcg
       61 gagagcagga tggtggtgac attcctcatg tcagctctcg agtccatgtg taaagaactg
      121 gccaagtcca aagccgaagt ggcctgcatt gcagtgtatg aaacagacgt gtttgtcgtc
      181 ggaactgaaa gaggacgtgc ttttgtcaat accagaaagg attttcaaaa agattttgta
      241 aaatattgtg ttgaagaaga agaaaaagct gcagagatgc ataaaatgaa atctacaacc
      301 caggcaaatc ggatgagtgt agatgctgta gaaattgaaa cactcagaaa aacagttgag
      361 gactatttct gcttttgcta tgggaaagct ttaggcaaat ccacagtggt acctgtacca
      421 tatgagaaga tgctgcgaga ccagtcggct gtggtagtgc aggggcttcc ggaaggtgtt
      481 gcctttaaac accccgagaa ctatgatctt gcaaccctga aatggatttt ggagaacaaa
      541 gcagggattt cattcatcat taagagacct tttttagagc caaagaagca tgtaggtggt
      601 cgtgtgatgg taacagatgc tgacaggtca atactatctc caggtggaag ttgtggcccc
      661 atcaaagtga aaactgaacc cacagaagat tctggcattt ccctggaaat ggcagctgtg
      721 acagtaaagg aagaatcaga agatcctgat tattatcaat ataacattca agcaggccct
      781 tctgaaactg atgatgttga tgaaaaacag cccctatcga agcctttgca aggaagccac
      841 cattcttcag agggcaatga aggcacagaa atggaagtac cagcagaaga ttctactcaa
      901 catgtccctt cagaaacaag tgaggaccct gaagttgagg tgactattga agatgatgat
      961 tattctccac cgtctaagag accaaaggcc aatgagctac cgcagccacc agtcccggaa
     1021 cccgccaatg ctgggaagcg gaaagtgagg gagttcaact tcgagaaatg gaatgctcgc
     1081 atcactgatc tacgtaaaca agttgaagaa ttgtttgaaa ggaaatatgc tcaagccata
     1141 aaagccaaag gtccggtgac gatcccgtac cctcttttcc agtctcatgt tgaagatctt
     1201 tatgtagaag gacttcctga aggaattcct tttagaaggc catctactta cggaattcct
     1261 cgcctggaga ggatattact tgcaaaggaa aggattcgtt ttgtgattaa gaaacatgag
     1321 cttctgaatt caacacgtga agatttacag cttgataagc cagcttcagg agtaaaggaa
     1381 gaatggtatg ccagaatcac taaattaaga aagatggtgg atcagctttt ctgcaaaaaa
     1441 tttgcggaag ccttggggag cactgaagcc aaggctgtac cgtaccaaaa atttgaggca
     1501 cacccgaatg atctgtacgt ggaaggactg ccagaaaaca ttcctttccg aagtccctca
     1561 tggtatggaa tcccaaggct ggaaaaaatc attcaagtgg gcaatcgaat taaatttgtt
     1621 attaaaagac cagaacttct gactcacagt accactgaag ttactcagcc aagaacgaat
     1681 acaccagtca aagaagattg gaatgtcaga attaccaagc tacggaagca agtggaagag
     1741 atttttaatt tgaaatttgc tcaagctctt ggactcaccg aggcagtaaa agtaccatat
     1801 cctgtgtttg aatcaaaccc ggagttcttg tatgtggaag gcttgccaga ggggattccc
     1861 ttccgaagcc ctacctggtt tggaattcca cgacttgaaa ggatcgtccg cgggagtaat
     1921 aaaatcaagt tcgttgttaa aaaacctgaa ctagttattt cctacttgcc tcctgggatg
     1981 gctagtaaaa taaacactaa agctttgcag tcccccaaaa gaccacgaag tcctgggagt
     2041 aattcaaagg ttcctgaaat tgaggtcacc gtggaaggcc ctaataacaa caatcctcaa
     2101 acctcagctg ttcgaacccc gacccagact aacggttcta acgttccctt caagccacga
     2161 gggagagagt tttcctttga ggcctggaat gccaaaatca cggacctaaa acagaaagtt
     2221 gaaaatctct tcaatgagaa atgtggggaa gctcttggcc ttaaacaagc tgtgaaggtg
     2281 ccgttcgcgt tatttgagtc tttcccggaa gacttttatg tggaaggctt acctgagggt
     2341 gtgccattcc gaagaccatc gacttttggc attccgaggc tggagaagat actcagaaac
     2401 aaagccaaaa ttaagttcat cattaaaaag cccgaaatgt ttgagacggc gattaaggag
     2461 agcacctcct ctaagagccc tcccagaaaa ataaattcat cacccaatgt taatactact
     2521 gcatcaggtg ttgaagacct taacatcatt caggtgacaa ttccagatga tgataatgaa
     2581 agactctcga aagttgaaaa agctagacag ctaagagaac aagtgaatga cctctttagt
     2641 cggaaatttg gtgaagctat tggtatgggt tttcctgtga aagttcccta caggaaaatc
     2701 acaattaacc ctggctgtgt ggtagttgat ggcatgcccc cgggggtgtc cttcaaagcc
     2761 cccagctacc tggaaatcag ctccatgaga aggatcttag actctgccga gtttatcaaa
     2821 ttcacggtca ttagaccatt tccaggactt gtgattaata accagctggt tgatcagagt
     2881 gagtcagaag gccccgtgat acaagaatca gctgaaccaa gccagttgga agttccagcc
     2941 acagaagaaa taaaagagac tgatggaagc tctcagatca agcaagaacc agaccccacg
     3001 tggtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     3061 tgtggaaagg acgaatgcgt catggatccg taactctt
//
