LOCUS       sequence-283344-        1475 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0983
FEATURES             Location/Qualifiers
     source          1..1475
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctacaag ggtgctgagc atgagcgccc gcctgggacc cgtgccccag
       61 ccgccggcgc cgcaggacga gccggtgttc gcgcagctca agccggtgct gggcgccgcg
      121 aatccggccc gcgacgcggc gctcttcccc ggcgaggagc tgaagcacgc gcaccaccgc
      181 ccgcaggcgc agcccgcgcc cgcgcaggcc ccgcagccgg cccagccgcc cgccaccggc
      241 ccgcggctgc ctccagagga cctggtccag acaagatgtg aaatggagaa gtatctgaca
      301 cctcagcttc ctccagttcc tataattcca gagcataaaa agtatagacg agacagtgcc
      361 tcagtcgtag accagttctt cactgacact gaagggttac cttacagtat caacatgaac
      421 gtcttcctcc ctgacatcac tcacctgaga actggcctct acaaatccca gagaccgtgc
      481 gtaacacaca tcaagacaga acctgttgcc attttcagcc accagagtga aacgactgcc
      541 cctcctccgg ccccgaccca ggccctccct gagttcacca gtatattcag ctcacaccag
      601 accgcagctc cagaggtgaa caatattttc atcaaacaag aacttcctac accagatctt
      661 catctttctg tccctaccca gcagggccac ctgtaccagc tactgaatac accggatcta
      721 gatatgccca gttctacaaa tcagacagca gcaatggaca ctcttaatgt ttctatgtca
      781 gctgccatgg caggccttaa cacacacacc tctgctgttc cgcagactgc agtgaaacaa
      841 ttccagggca tgcccccttg cacatacaca atgccaagtc agtttcttcc acaacaggcc
      901 acttactttc ccccgtcacc accaagctca gagcctggaa gtccagatag acaagcagag
      961 atgctccaga atttaacccc acctccatcc tatgctgcta caattgcttc taaactggca
     1021 attcacaatc caaatttacc caccaccctg ccagttaact cacaaaacat ccaacctgtc
     1081 agatacaata gaaggagtaa ccccgatttg gagaaacgac gcatccacta ctgcgattac
     1141 cctggttgca caaaagttta taccaagtct tctcatttaa aagctcacct gaggactcac
     1201 actggtgaaa agccatacaa gtgtacctgg gaaggctgcg actggaggtt cgcgcgatcg
     1261 gatgagctga cccgccacta ccggaagcac acaggcgcca agcccttcca gtgcggggtg
     1321 tgcaaccgca gcttctcgcg ctctgaccac ctggccctgc atatgaagag gcaccagaac
     1381 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1441 ggaaaggacg agggttatcc gctcgtcgga agtta
//
