LOCUS       sequence-283350-        2165 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1007
FEATURES             Location/Qualifiers
     source          1..2165
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaagcacg ttctatgctg gttccacccc aggcatctgt gtgcttcgag
       61 gatgtggcta tggcattcac acaggaggag tgggaacagc tggacctggc ccagaggaca
      121 ctgtaccgag aggtgacact ggagacctgg gagcatattg tctccctgga ctggaaagct
      181 acccttgagg agaataggtt gaattctgaa aaagatcgag ctagggaaga actatcccac
      241 cacgtggaag tgtacaggag tggaccggag gagccaccct ctttggtatt aggaaaagtg
      301 caagatcaga gcaaccagtt aagggaacac caggagaact ccttgaggtt catggtactc
      361 acctcagaga gactgtttgc tcaaagggaa cattgtgagc ttgaacttgg gggaggttat
      421 tctctacctt ctactttaag ccttctacct acaacattac ctacaagtac aggtttccct
      481 aagcccaact cacaagttaa agagttgaaa caaaattcag ctttcattaa tcatgagaaa
      541 aatggagcag atgggaagca ctgtgagagt catcagtgtg ctagagcttt ctgtcagagt
      601 atttacttga gtaaacttgg aaacgttgaa acaggaaaga aaaaccctta tgaatatatt
      661 gtcagtggtg actctctcaa ctatggttcc tccctttgtt ttcatggtag aactttttca
      721 gtgaagaaaa gtgatgactg taaggattat ggaaacctct tcagtcacag tgtgtctctg
      781 aatgaacaga agccagtgca ttttgggaaa agtcagtatg agtgtgatga gtgcagggaa
      841 acctgttctg agagtctgtg ccttgtacaa acagaaagaa gtggccctgg agagaccccc
      901 ttcagatgtg aggaacgctg tgctgccttc cccatggcct catctttttc tgactgtaac
      961 atcattcaga ctacagagaa gccatctgtg tgtaatcagt gtggaaaatc tttcagctgt
     1021 tgtaagctca tacaccagag aacacacact ggagaaaagc ccttcgaatg tactcagtgt
     1081 gggaaatctt ttagccagag ctatgacctt gtcatacatc agaggacaca cactggagag
     1141 aagccctatg agtgtgacct gtgtgggaaa tccttcaccc agagatccaa acttattaca
     1201 catcagcgaa ttcacactgg agaaaaaccg tatcagtgta ttgaatgcag aaaatccttc
     1261 aggtggaact ctaacctcat tgtacatcag agaattcata ctggagagaa accgtatgag
     1321 tgcactcact gtggaaagtc cttcagccaa agctatgagt tagttacaca taaaagaacg
     1381 cacactggag aaaaaccctt caaatgtact cagtgtggga aatctttcag ccagaagtat
     1441 gaccttgttg tacatcagag gacacacact ggagagaagc cctatgagtg caacctgtgt
     1501 gggaaatcct tctcccagag ttccaaactt attacgcatc agcgaattca cactggagaa
     1561 aaaccgtatc agtgtattga atgtgggaaa tccttcagat ggaactctaa cctcgtcata
     1621 catcagagaa ttcatactgg agagaaaccg tacgattgca ctcactgtgg aaagtccttc
     1681 agccaaagct atcagttagt tgcacataaa agaactcaca ctggagaaaa gccctatgaa
     1741 tgtaacgagt gtggaaaagc cttcaatcga agcactcagc tcatcaggca tctgcaaatt
     1801 cacactgggg agaagccgta caaatgcaat cagtgcaata aagcctttgc aaggagctcc
     1861 taccttgtga tgcatcagag aactcacact ggtgagaaac cttttgagtg tagtcagtgt
     1921 gggaaagcct tttcagggag ctctaacctt ctttcccatc acagaattca ttctggagag
     1981 aaaccctatg aatgtagtga ctgtgggaaa tccttccggc agcaatctca acttgtagtg
     2041 catcggcgga cacatactgg agagaaacct tagtaatgaa ctagtccgta acttgaaagt
     2101 atttcgattt cttggcttta tatatcttgt ggaaaggacg atagtaggtt gcgtaagccg
     2161 tccca
//
