LOCUS       sequence-283353-        1310 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1010
FEATURES             Location/Qualifiers
     source          1..1310
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagaaat gaaccctgca cagggtcatg tggtttttga agacgtggcc
       61 atatatttct cccaggagga gtgggggcat ctcgatgagg ctcagagatt gctgtaccgt
      121 gatgtgatgc tggagaattt ggcccttttg tcctcactag gttgttgcca tggagctgag
      181 gatgaggagg cacctttaga gccaggtgtt tctgtaggag tgtcacaggt catggctcca
      241 aagccctgtc tatctaccca gaatacccag ccctgtgaga catgtagctc acttctgaag
      301 gacattctgc gtctggctga gcatgacgga acacaccccg agcagggact gtacacgtgt
      361 ccagcacatc ttcaccagca ccaaaaggag cagattagag agaaactttc tagaggggat
      421 ggaggaagac cgacatttgt gaagaaccac agagttcaca tggcagggaa gaccttcttg
      481 tgcagtgaat gtgggaaagc ctttagccac aaacataaac tttctgacca tcagaaaatc
      541 cacactggag aaagaactta taagtgcagc aaatgtggga tattgtttat ggaaaggtcc
      601 acactcaata gacatcagag aactcacact ggagaaaggc cttatgagtg caatgaatgt
      661 gggaaagcct ttctttgtaa gtctcacctt gttcgtcacc agacaatcca ctctggagaa
      721 aggccttatg agtgcagtga atgtgggaaa ttgtttatgt ggagttccac actcattaca
      781 catcagaggg ttcacactgg aaagaggcct tatggttgca gtgaatgtgg gaagttcttt
      841 aagtgcaact caaacctctt taggcattac agaattcata caggaaaaag gtcttatggt
      901 tgcagtgaat gtgggaaatt ctttatggaa aggtctacac tcagtagaca tcagagagtt
      961 cacactggag aaaggcctta tgagtgcaat gaatgtggga aattcttcag cttgaaatcc
     1021 gtcctcattc aacaccaaag agttcacact ggagaacggc cttatgaatg cagtgagtgt
     1081 gggaaggcct tccttacaaa gtcccacctc atttgtcatc agacagttca cactgcagca
     1141 aagcagtgca gtgaatgtgg gaaattcttt aggtataact ctacacttct cagacatcag
     1201 aaagtccaca ctggatagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1261 ctttatatat cttgtggaaa ggacgaccag taccagcacg tctacaagct
//
