LOCUS       sequence-283365-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1035
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagaaggg ttacagcacg gtcaccttcg acgggacgcc cagctacggt
       61 cacacgccct cgcaccatgc ggcgcagttc cccaaccact cattcaagca tgaggatccc
      121 atgggccagc agggctcgct gggtgagcag cagtactcgg tgccgccccc ggtctatggc
      181 tgccacaccc ccaccgacag ctgcaccggc agccaggctt tgctgctgag gacgccctac
      241 agcagtgaca atttatacca aatgacatcc cagcttgaat gcatgacctg gaatcagatg
      301 aacttaggag ccaccttaaa gggccacagc acagggtacg agagcgataa ccacacaacg
      361 cccatcctct gcggagccca atacagaata cacacgcacg gtgtcttcag aggcattcag
      421 gatgtgcgac gtgtgcctgg agtagccccg actcttgtac ggtcggcatc tgagaccagt
      481 gagaaacgcc ccttcatgtg tgcttaccca ggctgcaata agagatattt taagctgtcc
      541 cacttacaga tgcacagcag gaagcacact ggtgagaaac cataccagtg tgacttcaag
      601 gactgtgaac gaaggttttc tcgttcagac cagctcaaaa gacaccaaag gagacataca
      661 ggtgtgaaac cattccagtg taaaacttgt cagcgaaagt tctcccggtc cgaccacctg
      721 aagacccaca ccaggactca tacaggtaaa acaagtgaaa agcccttcag ctgtcggtgg
      781 ccaagttgtc agaaaaagtt tgcccggtca gatgaattag tccgccatca caacatgcat
      841 cagagaaaca tgaccaaact ccagctggcg ctttagtaat gaactagtcc gtaacttgaa
      901 agtatttcga tttcttggct ttatatatct tgtggaaagg acgataggat tgccgttgac
      961 atcagtac
//
