LOCUS       sequence-283371-        4553 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1046
FEATURES             Location/Qualifiers
     source          1..4553
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(3655..3666)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accgccacca tggcgccgct cctgggccgc aagcccttcc cgctggtgaa gccgttgccc
       61 ggagaggagc cgctcttcac catcccgcac actcaggagg ccttccgcac ccgggaagag
      121 tatgaagccc gcttggaaag gtacagtgag cgcatttgga cgtgcaagag tactggaagc
      181 agtcagctaa cacacaagga agcctgggag gaagaacagg aagttgctga gcttttgaag
      241 gaggagtttc ctgcctggta tgagaagctt gttctggaaa tggttcacca taacacagcc
      301 tccttagaga agttagtaga tactgcttgg ttggagatca tgaccaaata tgctgtggga
      361 gaagagtgtg acttcgaggt tgggaaggag aaaatgctca aggtgaagat tgtgaagatt
      421 catcctttgg agaaagtgga tgaagaggcc actgagaaga aatctgatgg tgcctgtgat
      481 tctccatcaa gtgacaaaga gaactccagt cagattgctc aggaccatca gaagaaggag
      541 acagttgtga aagaggatga aggaaggaga gagagtatta atgacagagc acgtagatcg
      601 ccacgaaaac ttcctacttc attaaaaaaa ggagaaagga aatgggctcc tccaaaattt
      661 ctgcctcaca aatatgatgt gaaactacaa aatgaagata agatcatcag taacgtgcca
      721 gcagacagct tgattcgtac agagcgccca ccaaataagg agatagttcg atactttata
      781 cggcataatg cattacgagc tggtactggt gaaaatgcac cttgggtcgt agaagatgaa
      841 ttggtgaaga aatactctct gcccagcaag ttcagtgact ttttacttga tccatacaag
      901 tatatgactc tcaacccttc tactaagagg aagaatactg gatccccaga caggaagccc
      961 tcaaagaaat ccaagacaga caactcttct cttagttcac cactaaatcc taagttatgg
     1021 tgtcacgtac acttgaagaa gtcattgagt ggctcgccac tcaaagtgaa gaactcaaag
     1081 aattccaaat ctcctgaaga acatctagaa gaaatgatga agatgatgtc gcccaataag
     1141 ctgcacacta actttcacat tcctaaaaaa ggcccacctg ccaagaaacc agggaagcac
     1201 agtgacaagc ctttgaaggc aaagggcaga agcaaaggca tcctgaatgg acagaaatcc
     1261 acagggaatt ccaaatctcc caaaaaagga ctgaagactc ctaaaaccaa aatgaagcag
     1321 atgactttgt tggatatggc caaaggcacg cagaagatga cacgagcccc acggaattct
     1381 gggggtacac ctaggacctc tagtaaacct cataaacatc tgcctcctgc agccctacac
     1441 ctcattgcat actacaaaga aaacaaagac agggaggaca agaggagcgc cctgtcctgt
     1501 gttatctcca aaacagctcg tcttctctct agtgaagata gagctcgtct cccagaagaa
     1561 ttgcgaagtc ttgttcaaaa acgctatgaa cttctagagc acaaaaagag gtgggcttct
     1621 atgtctgaag aacaacggaa agaatatttg aaaaagaaac gggaggagct gaaaaagaag
     1681 ttgaaggaaa aagccaaaga acgaagagag aaagaaatgc ttgagagatt agaaaaacag
     1741 aagcggtatg aggaccaaga gttaactggc aaaaaccttc cagcattcag attggtggat
     1801 acccctgaag ggctgcccaa cacgctgttt ggggatgtgg ccatggtggt ggaattcttg
     1861 agctgttatt ctgggctact tttaccagat gctcagtatc ctattactgc tgtgtccctt
     1921 atggaagcct tgagtgcaga taagggtggc tttttatacc ttaacagggt gttggtcatc
     1981 ctcttacaga ccctcctaca agatgagata gcagaagact atggtgaatt gggaatgaag
     2041 ctgtcggaaa tccccttgac tctgcattct gtttcagagc tggtgcggct ctgcttgcgc
     2101 agatctgatg ttcaggagga aagcgagggc tcagacacag atgacaataa agattcagct
     2161 gcatttgagg ataatgaggt acaagatgag ttcctagaaa agctggagac ctctgaattt
     2221 tttgagctga cgtcagagga gaagctacag atcttgacag cactgtgcca ccggatcctc
     2281 atgacatact cagtgcaaga ccacatggag accagacagc agatgtctgc agagttgtgg
     2341 aaggaacggc ttgctgtgtt gaaggaagaa aatgataaga agagagcaga gaaacagaaa
     2401 cggaaagaaa tggaagccaa aaataaagaa aatggaaaag ttgagaatgg gttaggcaaa
     2461 actgatagga aaaaagaaat tgtgaagttt gagccccaag tagatacaga agctgaagac
     2521 atgattagtg ctgtgaagag cagaaggttg cttgccattc aagctaagaa ggaacgggaa
     2581 atccaggaaa gagaaatgaa agtgaaactg gaacgccaag ctgaagaaga acgaatacgg
     2641 aagcacaaag cagctgctga gaaagctttc caggaaggga ttgccaaggc caaactagtc
     2701 atgcgcagga ctcctattgg cacagatcga aaccataata gatactggct cttctcagat
     2761 gaagttccag gattattcat tgaaaaaggc tgggtacatg acagcattga ctaccgattc
     2821 aaccatcact gcaaagacca cacagtctct ggtgatgagg attactgtcc tcgcagtaag
     2881 aaagcaaact taggtaaaaa tgcaagcatg aacacacaac atggaacagc aacagaagtt
     2941 gctgtagaga caaccacacc caaacaagga cagaacctat ggtttttatg tgatagtcaa
     3001 aaggagctgg atgagttgct aaactgtctt caccctcagg gaataagaga aagtcaactt
     3061 aaagagagac tagagaagag gtaccaggac attattcact ctattcatct agcacggaag
     3121 ccaaatttgg gtctaaaatc ttgtgatggc aaccaggagc ttttaaactt ccttcgtagt
     3181 gatctcattg aagttgcaac aaggttacaa aaaggaggac ttggatatgt ggaagaaaca
     3241 tcagaatttg aagcccgggt catttcatta gagaaattga aggattttgg tgagtgtgtg
     3301 attgcccttc aggccagtgt cataaagaaa tttctccaag gcttcatggc tcccaagcaa
     3361 aagagaagaa aactccaaag tgaagattca gcaaaaactg aggaagtgga tgaagagaag
     3421 aaaatggtag aggaagcaaa ggttgcatct gcactggaga aatggaagac agcaatccgg
     3481 gaagctcaga ctttctccag gatgcacgtg ctgcttggga tgcttgatgc ctgtatcaag
     3541 tgggatatgt ccgcagaaaa tgctaggtgc aaagtttgtc gaaagaaagg tgaggatgac
     3601 aaattgatct tgtgtgatga gtgtaataaa gccttccacc tgttttgtct gaggccggcc
     3661 ctctatgaag taccagatgg tgagtggcag tgcccagctt gccagcccgc tactgccagg
     3721 cgcaactccc gtggcaggaa ctatactgaa gagtctgctt ctgaggacag tgaagatgat
     3781 gagagtgatg aagaggagga ggaggaagaa gaggaggagg aggaagaaga ttatgaggtg
     3841 gctggtttgc gattgagacc tcgaaagacc atccggggca agcacagcgt catcccccct
     3901 gcagcaaggt caggccggcg cccgggtaag aagccacact ctaccaggag gtctcagccc
     3961 aaggcaccac ctgtggatga tgctgaggtg gatgagctgg tgcttcagac caagcggagc
     4021 tcccggaggc aaagcctgga gctgcagaag tgtgaagaga tcctccacaa gatcgtgaag
     4081 taccgcttca gctggccctt cagggagcct gtgaccagag atgaggccga ggactactat
     4141 gatgtgatca cgcaccccat ggactttcag acagtgcaga acaaatgttc ctgtgggagc
     4201 taccgctctg tgcaggagtt tcttactgac atgaagcaag tgtttaccaa tgctgaggtt
     4261 tacaactgcc gtggcagcca tgtgctaagc tgcatggtga agacagaaca gtgtctagtg
     4321 gctctgttgc ataaacacct tcctggccac ccatatgtcc gcaggaagcg caagaagttt
     4381 cctgataggc ttgctgaaga tgaaggggac agtgagccag aggccgttgg acagtccagg
     4441 ggacgaagac agaagaagta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     4501 tggctttata tatcttgtgg aaaggacgaa agctaacaat aggaaatggc att
//
