LOCUS       sequence-283389-        2486 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1075
FEATURES             Location/Qualifiers
     source          1..2486
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggggcggc cagctgcgag gatgaggagc tggaatttaa gctggtgttc
       61 ggggaggaaa aggaggcccc cccgctgggc gcggggggat tgggggaaga actggactca
      121 gaggatgccc cgccatgctg ccgtctggcc ttgggagagc cccctcccta tggcgctgca
      181 cctatcggta ttccccgacc tccaccccct cggcctggca tgcattcgcc accgccgcga
      241 ccagccccct cacctggcac ctgggagagc cagcccgcca ggtcggtgag gctgggagga
      301 ccaggagggg gtgctggggg tgctgggggt ggccgtgttc tcgagtgtcc cagcatccgc
      361 atcacctcca tctctcccac gccggagccg ccagcagcgc tggaggacaa ccctgatgcc
      421 tggggggacg gctctcctag agattacccc ccaccagaag gctttggggg ctacagagaa
      481 gcagggggcc agggtggggg ggccttcttc agcccaagcc ctggcagcag cagcctgtcc
      541 tcgtggagct tcttctccga tgcctctgac gaggcagccc tgtatgcagc ctgcgacgag
      601 gtggagtctg agctaaatga ggcggcctcc cgctttggcc tgggctcccc gctgccctcg
      661 ccccgggcct cccctcggcc atggaccccc gaagatccct ggagcctgta tggtccaagc
      721 cccggaggcc gagggccaga ggatagctgg ctactcctca gtgctcctgg gcccacccca
      781 gcctccccgc ggcctgcctc tccatgtggc aagcggcgct attccagctc gggaacccca
      841 tcttcagcct ccccagctct gtcccgccgt ggcagcctgg gggaagaggg gtctgagcca
      901 cctccaccac ccccattgcc tctggcccgg gacccgggct cccctggtcc ctttgactat
      961 gtgggggccc caccagctga gagcatccct cagaagacac ggcggacttc cagcgagcag
     1021 gcagtggctc tgcctcggtc tgaggagcct gcctcatgca atgggaagct gcccttggga
     1081 gcagaggagt ctgtggctcc tccaggaggt tcccggaagg aggtggctgg catggactac
     1141 ctggcagtgc cctccccact cgcttggtcc aaggcccgga ttgggggaca cagccctatc
     1201 ttcaggacct ctgccctacc cccactggac tggcctctgc ccagccaata tgagcagctg
     1261 gagctgagga tcgaggtaca gcctagagcc caccaccggg cccactatga gacagaaggc
     1321 agccgtggag ctgtcaaagc tgcccctggc ggtcaccccg tagtcaagct cctaggctac
     1381 agtgagaagc cactgaccct acagatgttc atcggcactg cagatgaaag gaacctgcgg
     1441 cctcatgcct tctatcaggt gcaccgtatc acaggcaaga tggtggccac ggccagctat
     1501 gaagccgtag tcagtggcac caaggtgttg gagatgactc tgctgcctga gaacaacatg
     1561 gcggccaaca ttgactgcgc gggaatcctg aagcttcgga attcagacat tgagcttcgg
     1621 aagggtgaga cggacatcgg gcgcaaaaac acacgtgtac ggctggtgtt ccgggtacac
     1681 gtgccccagg gcggcgggaa ggtcgtctca gtacaggcag catcggtgcc catcgagtgc
     1741 tcccagcgct cagcccagga gctgccccag gtggaggcct acagccccag tgcctgctct
     1801 gtgagaggag gcgaggaact ggtactgact ggctccaact tcctgccaga ctccaaggtg
     1861 gtgttcattg agaggggtcc tgatgggaag ctgcaatggg aggaggaggc cacagtgaac
     1921 cgactgcaga gcaacgaggt gacgctgacc ctgactgtcc ccgagtacag caacaagagg
     1981 gtttcccggc cagtccaggt ctacttttat gtctccaatg ggcggaggaa acgcagtcct
     2041 acccagagtt tcaggtttct gcctgtgatc tgcaaagagg agcccctacc ggactcatct
     2101 ctgcggggtt tcccttcagc atcggcaacc ccctttggca ctgacatgga cttctcacca
     2161 cccaggcccc cctacccctc ctatccccat gaagaccctg cttgcgaaac tccttaccta
     2221 tcagaaggct tcggctatgg catgccccct ctgtaccccc agacggggcc cccaccatcc
     2281 tacagaccgg gcctgcggat gttccctgag actaggggta ccacagtgag tgagatcatt
     2341 ggccgagacc tgagtggctt ccctgcacct cctggagaag agcctcctgc ctagtaatga
     2401 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     2461 gatatgagcg cttgcacatc ctttag
//
