LOCUS       sequence-28339-e         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-BAP1
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..25
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             38..64
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    80..104
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggactacaag gatgacgatg acaagctcga tggaggatac ccatacgatg ttccagatta
       61 cgctgctcga ggttatcaaa caagtttgta caaaaaagtt ggcatgaata agggctggct
      121 ggagctggag agcgacccag gcctcttcac cctgctcgtg gaagatttcg gtgtcaaggg
      181 ggtgcaagtg gaggagatct acgaccttca gagcaaatgt cagggccctg tatatggatt
      241 tatcttcctg ttcaaatgga tcgaagagcg ccggtcccgg cgaaaggtct ctaccttggt
      301 ggatgatacg tccgtgattg atgatgatat tgtgaataac atgttctttg cccaccagct
      361 gatacccaac tcttgtgcaa ctcatgcctt gctgagcgtg ctcctgaact gcagcagcgt
      421 ggacctggga cccaccctga gtcgcatgaa ggacttcacc aagggtttta gccctgagag
      481 caaaggatat gcgattggca atgccccgga gttggccaag gcccataata gccatgccag
      541 gcccgagcca cgccacctcc ctgagaagca gaatggcctt agtgcagtgc ggaccatgga
      601 ggcattccac tttgtcagct atgtgcctat cacaggccgg ctctttgagc tggatgggct
      661 gaaggtctac cccattgacc atgggccctg ggggggagga cgaggagtgg acaga
//
