LOCUS       sequence-283398-        2081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1081
FEATURES             Location/Qualifiers
     source          1..2081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctctcct tttgacacta acatcaccag acctggaggg tacctgggac
       61 accagagaca aggatggctt caaagcccag gaggggccgc ccctggctgt acctgaattt
      121 cctgtgtgtg gactgtaccg gatctacgga gtctgtggct ctttcagctc cttcttcatc
      181 atccggtgca gcctttgtgc tctggagacc ctcaaatcac cccaacatga tcccttagag
      241 atacctgaac agtccctcaa actcataccc ctggtgtctg ggaaaaggga actcacaagg
      301 ggccagaaag ctggagagaa gcccctggct gcaggacccg gggaggagga actgctccgg
      361 ggctcagccc ctcatgctca ggacactcag agtgaggaac tgccaccctc ctgcaccatc
      421 tcaggagaga agaagccgcc agcagtctct ggagaagcca ccggggctga tgctgggaga
      481 ctgtgcccgc ccccccgctc cagggctccc cacaaagaca gaactctagc ccgctccagg
      541 ccccagactc agggggaaga ttgttccctc ccagtgggag aggtgaagat aggaaagagg
      601 tcctattctc cagcccccgg gaagcagaaa aagcctaatg ccatgggtct ggccccaaca
      661 tcatctccgg gtgcccctaa ctcagcccgt gccacacaca acccagtgcc ctgtgggtca
      721 ggccgggggc cctgccacct ggccaatctc ctcagtacat tggcgcagag caaccaaaac
      781 agagaccaca agcaggggcc cccggaagtg acctgccaaa ttaggaaaaa gacacgaacc
      841 ctataccgct cagatcagct ggaggagcta gagaagatat tccaagaaga ccactatcct
      901 gacagtgata aacgccgaga gattgcccag acggtggggg tgacccccca gcgcatcatg
      961 gtgtggttcc agaatcgccg ggccaagtgg cgaaaaatgg agaaactgaa tgggaaagaa
     1021 agcaaggaca atcctgcagc ccctggccct gccagcagtc aatgcagctc tgcagctgag
     1081 atcctacctg ctgtgcccat ggagccaaag cctgaccctt tccctcagga gtcccctctg
     1141 gatacctttc cagagccccc catgctgctg acttctgacc agactttggc ccccacccaa
     1201 cccagtgagg gtgctcagag ggtggtgacc cccccactct tcagcccccc acctgtgcga
     1261 agggccgatc ttcctttccc ccttggccct gtccacaccc cccaactgat gccactgctg
     1321 atggatgttg ctggcagtga cagcagccac aaggacggcc cctgtgggtc ctgggggaca
     1381 agcatcaccc tgccaccccc ctgttcatat ttggaggagc tggagcccca ggattaccaa
     1441 cagagcaacc agccaggacc cttccagttc tcccaggctc cacagccccc gcttttccag
     1501 tcccctcagc ccaagttgcc ctacctcccc actttcccct tctccatgcc cagttcactg
     1561 acgcttccac cgcccgaaga ctctctcttt atgtttccct gtggccccag cgggggcaca
     1621 tcgcagggct attgcccagg tgcctcctca ggacagatcc tgatgcaacc acctgctggg
     1681 aatataggta cagcctcctg gagtgacccc tgtttgccag agctgccctt ccctggtccg
     1741 ttctgcccac aagctctggg gcatccccca ggaggggatg gctactttcc tgatctattt
     1801 ccaactccct gcccccaggc tctgggcagg cagccttcgt cagctctctc atggatgcct
     1861 gaaggggcca gaccagggac tgggccctta ctcagcaagg caaaagagga accaccagct
     1921 gcttccctgg atcagccctc agcactggag gaggccagag gggatgacaa gaatagccat
     1981 gtcccctagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     2041 tcttgtggaa aggacgatat cctgtggaat ctcgggagga g
//
