LOCUS       sequence-28340-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-BRCC3
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..78
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     94..110
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      139..148
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             148..171
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             184..210
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    226..250
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc tctccccctt
       61 gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac tccttctcta
      121 ggcgccggaa ttagatctgc caccatggac tacaaggatg acgatgacaa gctcgatgga
      181 ggatacccat acgatgttcc agattacgct gctcgaggtt atcaaacaag tttgtacaaa
      241 aaagttggca tggcggtgca ggtggtgcag gcggtgcagg cggttcatct cgagtctgac
      301 gctttcctcg tttgtctcaa ccacgctctg agcacagaga aggaggaagt aatggggctg
      361 tgcatagggg agttgaacga tgatacaagg agtgactcca aatttgcata tactggaact
      421 gaaatgcgca cagttgctga aaaggttgat gccgtcagaa ttgttcacat tcattctgtc
      481 atcatcttac gacgttctga taagaggaag gaccgagtag aaatttctcc agagcagctg
      541 tctgcagctt caacagaggc agagaggttg gctgaactga caggccgccc catgagagtt
      601 gtgggctggt atcattccca tcctcatata actgtttggc cttcacatgt tgatgttcgc
      661 acacaagcca tgtaccagat gatggatcaa ggctttgtag gacttatttt ttcctgtttc
      721 atagaagana agaacacaaa gactggccgg gtactctaca cttgcttcca atccatacag
      781 gcccaaaaga gttcagagtc ccttcatggt ccacgagact tctggagctc cagccagcac
      841 atctccattg agggccagaa ggaagangaa aggnatgana gaatcgaaat nccaatc
//
