LOCUS       sequence-283404-        1589 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1093
FEATURES             Location/Qualifiers
     source          1..1589
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagatgca atcctattat gccaagcttt tgggggagct gaatgaacag
       61 agaaagaggg actttttctg tgactgcagc atcattgtgg aagggcggat cttcaaggcc
      121 cacaggaaca ttttgtttgc taacagcggc tacttccgag ccctgctcat tcactatatc
      181 caggacagcg ggcggcatag caccgcctcc ttggacattg tcacctctga tgccttctcc
      241 atcatcttag atttcctcta ttctgggaag ttggatttgt gtggggagaa tgtgattgaa
      301 gtgatgtcgg ctgccagcta cctgcagatg aatgacgtgg tgaacttctg caagacatac
      361 attaggtcat ccctcgacat ttgccgaaag atggagaagg aggctgctgt ggctgcagca
      421 gtggcggcgg cagcggcggc ggctgcagcg gcggcagcag cggcggctca tcaggttgac
      481 agtgaaagcc ccagttcagg ccgggagggg acctcctgtg gtaccaagag cttggtctcc
      541 tctccagccg agggagaaaa gagcgtggag tgcctgagag agtccccttg cggtgactgc
      601 ggagactgcc accccttgga actggtggtg agagacagcc ttggcggtgg ctcggctgac
      661 agcaacctct ctactccacc caaacggata gagcccaagg tggaatttga tgctgatgaa
      721 gtggaggtgg acgttggtga acagctgcag cagtatgctg ccccgctgaa cctggcccac
      781 gtggaggagg ccttgccaag cggccaggcg gttgacttgg cttacagcaa ctaccacgtg
      841 aagcagttcc tggaggcgct cttgcgcaac agcgctgccc cgagcaagga tgatgcagac
      901 catcactttt ctaggagttt ggaaggaaga ccagaaggtg caggagtagc catgagttcc
      961 atgatggatg tccaggctga ctggtatgga gaggactcag gtgatgtgct ggtggtcccc
     1021 atcaagctcc acaagtgtcc tttctgccct tacactgcca aacagaaggg catcctcaag
     1081 cggcacatcc gttcacacac aggcgagcgg ccctacccct gtgagacctg cggcaagagg
     1141 ttcactcgac aagagcacct gcggagccac gcactgagtg tccacagatc caaccgtcca
     1201 atcatctgca agggctgcag gagaacattc acgagtcacc tgtcccaggg gctgcggcgc
     1261 ttcgggctgt gtgacagctg cacctgcgtt acagacacac ccgatgatga tgatgatttg
     1321 atgcccatca accttagctt ggtggaggct tcatctgaaa gccaagaaaa gagcgacaca
     1381 gacaatgact ggccaatcta tgtggagtcg ggtgaggaaa atgaccctgc tggagatgat
     1441 tctgatgaca aaccacaaat tcagcctaac ttatcagacc gagagacact tacgtagtaa
     1501 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1561 gacgatgcac cgtacgtgtt gcccaccta
//
