LOCUS       sequence-283422-        2837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1124
FEATURES             Location/Qualifiers
     source          1..2837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggtaaa ttgtttaaca ctaaaagacc tgatcagccc caggcagccc
       61 agactagatt ttgcagttga agatggggaa aatgcacaaa aggaaaatat atttgttgat
      121 cgatcaagga tggccccgaa gactccaata aaaaatgaac caattgattt atcgaagcaa
      181 aaaaaattta ctccagaaag aaatcccatt actccagtta agtttgttga cagacagcaa
      241 gcggaaccat ggacacccac agctaacctg aagatgctca ttagtgctgc cagcccagat
      301 ataagggacc gggagaagaa aaagggacta ttccgaccca ttgaaaacaa ggacgatgca
      361 tttacagatt ctctacagct tgatgttgtt ggggacagtg ctgtggacga atttgaaaag
      421 caaaggccaa gcagaaaaca gaaaagttta ggactcctgt gccagaagtt tctagctcgc
      481 tatccaagtt atcccttgtc aactgagaaa actaccatct ccctagatga agttgctgtc
      541 agtcttggtg tggaaaggag acgcatctat gacattgtaa atgtgctgga gtcgctgcat
      601 ctggtcagcc gggtggctaa gaatcagtat ggctggcatg gacggcacag cctgccaaaa
      661 accctgagga acctccagag actaggagag gagcagaaat atgaagagca aatggcctac
      721 ctccaacaga aagagctgga cctgatagat tataaatttg gagaacgtaa aaaagatggt
      781 gatccagatt cccaggaaca acagttactg gatttctctg aacccgactg tccctcttca
      841 tctgcaaaca gtagaaaaga caagtctctg agaattatga gccagaagtt tgtcatgctg
      901 ttcctcgtct ccaaaaccaa gattgtcact ctggatgtgg ctgccaaaat actgatagaa
      961 gaaagccaag atgccccaga ccatagtaaa tttaaaacaa aggtacgacg cctctatgac
     1021 atagccaatg ttctgaccag cttggctctg ataaagaaag tgcatgtaac agaagagcga
     1081 ggtcgtaaac cagccttcaa gtggatcggg cctgtggact tcagctcaag tgatgaagaa
     1141 ctggtggatg tttctgcatc tgtcttacca gaattgaaaa gagaaacata tggccagatt
     1201 caagtctgtg caaaacagaa gctggctcgc catggttctt ttaacacagt tcaggcttct
     1261 gagaggatcc agaggaaagt gaactcagaa ccgagcagcc cgtacagaga agaacaagga
     1321 tcaggtggct actctttaga aattggaagc ctggcagctg tctatagaca gaaaatagaa
     1381 gacaattcac agggaaaagc ctttgccagt aagagagtgg tgcctccatc aagcagcttg
     1441 gaccctgttg ctcctttccc tgtcctctct gttgacccag aatattgtgt taatccttta
     1501 gcccacccag tattttctgt tgctcagacg gacctgcagg cattctccat gcagaacggt
     1561 ctgaatggac aagtggatgt ctcacttgct tctgcagcct ctgctgtgga gagcctgaag
     1621 ccagcactcc ttgctggcca gcctctagtg tatgtgccct ctgcctcact gttcatgctg
     1681 tatggaagtc tgcaggaggg accagcgtca gggtcagggt cagagaggga tgacagaagc
     1741 tcagaagccc cagccacagt agagctgtca tctgcaccct cagctcagaa gcgcctctgt
     1801 gaggagagga aacctcagga ggaggatgag ccagccacta aaaggcaaag tagggaatat
     1861 gaagacggcc cgctgtcgct tgtcatgccc aagaaaccct cagattccac agaccttgcc
     1921 tctcccaaga ctatgggtaa cagggcatct atacccctca aagacattca tgtgaatggc
     1981 caactccctg ctgcagaaga gatttcagga aaggcaacag caaactctct tgtttcttct
     2041 gagtggggaa atccttcaag aaatacagat gttgaaaagc cttcaaaaga aaatgaaagc
     2101 accaaagagc cttctttgct acaatatctt tgtgtgcagt ctcctgcagg attaaatggt
     2161 ttcaatgtac ttttatctgg cagtcaaacc ccccctactg tgggcccgtc ctcaggtcag
     2221 ctgccgtctt tcagtgtccc ttgcatggtc ttaccatctc cacctctggg cccttttcct
     2281 gttctctatt ctcctgcaat gccgggcccg gtttcttcta ctcttggtgc tctcccaaac
     2341 acaggacctg tgaatttcag cttgcctggc cttggatcaa tagcccagct tctcgtcggc
     2401 cccacagctg tggttaatcc aaagtcgtcc acactccctt ctgcagaccc tcagcttcag
     2461 agtcagccct cactaaacct aagtccagtg atgtcaaggt cacacagtgt cgtccaacaa
     2521 cctgagtccc ccgtttacgt gggacatcca gtctcagtag taaaattaca tcagtcacca
     2581 gttccagtga cccccaagag catccaacgc acacatcgtg agacgttttt caagacaccc
     2641 ggcagccttg gagaccctgt cctgaagaga agagaaagga accagtcacg aaacaccagc
     2701 tcggcccaga ggagactaga aatccccagc ggcggcgctg actagtaatg aactagtccg
     2761 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgacaggttt
     2821 gcgtaagtgc gcggctg
//
