LOCUS       sequence-283449-        1754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1238
FEATURES             Location/Qualifiers
     source          1..1754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             549..560
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(1622..1645)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 accgccacca tggtgcgagg ctgggagccg ccgcccgggc tggactgcgc tatctctgaa
       61 gggcacaaat cagaaggcac catgcctcct aataaagagg ccagcggtct cagtagttca
      121 ccagcggggc tcatctgcct ccctccaatc tctgaggagc tacagcttgt gtggacgcaa
      181 gcagcccaga ccagtgagct agacagcaat gaacacctgc tcaaaacctt cagctacttt
      241 ccctatccca gcctagcaga cattgccctt ctctgcctac gttatgggtt gcagatggag
      301 aaagtcaaga cttggtttat ggcccagcgc ctccgctgtg gtattagctg gtcatctgaa
      361 gaaatagaag agactcgagc ccgagtagtc taccgtcggg accaactcca tttcaaatcc
      421 cttctctctt ttactcatca tgcgggacgg cccccagagg aggtgcctcc tcctccagtg
      481 ccagctccag agcaagttgg tattggaata ggtcctccaa ctcttagcaa gcctacccag
      541 acgaaaggat tgaaggtaga gcctgaggag ccctctcaga tgccaccact gccacagagt
      601 caccagaaat taaaggagtc cctgatgaca cctggcagtg gagcattccc ctaccaatca
      661 gatttttggc aacatcttca aagcagtggc ctctcaaagg agcaggcagg caggggtccc
      721 aaccagtcac atggcatagg tactgcttcc tggaaccact ccacaaccgt cccccagcca
      781 caagctcggg ataaaccccc accaattgca ttaattgcca gtagttgtaa ggaggagtca
      841 gcatctagtg ttactccctc ttcttcctct acctcttctt ctttccaggt actggctaat
      901 ggagctactg ccgcctctaa acccctccag ccactaggct gtgtcccaca gtcagtgtca
      961 cccagtgaac aggcattacc cccacatctg gaaccagcct ggccccaagg gctacggcat
     1021 aactcagtac caggtagagt tggccccaca gagtaccttt ccccagatat gcaacgccag
     1081 cgaaagacca agcgcaaaac caaagagcag ctggctatcc ttaaatcctt ttttttacag
     1141 tgccagtggg cacggcgtga ggattaccaa aagttagaac agatcactgg tttacctcgg
     1201 cctgagatca ttcagtggtt tggtgacaca cgctatgcct tgaagcatgg gcaactaaag
     1261 tggtttcggg acaacgcagt acctggtgcc cctagtttcc aagacccagc aattcctaca
     1321 ccaccaccat caacccgctc cttgaacgaa agggctgaga caccacctct gccgatccct
     1381 ccacccccac cggatataca acccttggag aggtactggg cagcccacca acagctacgg
     1441 gaaactgata tccctcaatt gagtcaggca tcaaggctta gcacccagca ggtgctggat
     1501 tggtttgact ctcgattacc tcagccagct gaggtggtag tttgtctaga tgaagaagag
     1561 gaagaggagg aggaagaact gccagaagat gatgaggaag aggaggagga ggaggaggaa
     1621 gatgatgatg atgatgatga tgatgtgatc atacaagact agtaatgaac tagtccgtaa
     1681 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga ttcgacaata
     1741 tcgcagctgg atca
//
