LOCUS       sequence-28345-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-CYLD
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     63..85
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     101..117
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      146..155
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             155..178
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             191..217
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    233..257
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gggtcagccc tttgtacncc ctaagcctcc gcctcctctt cctccatccg ccccgtctct
       61 cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag ccctcactcc
      121 ttctctaggc gccggaatta gatctgccac catggactac aaggatgacg atgacaagct
      181 cgatggagga tacccatacg atgttccaga ttacgctgct cgaggttatc aaacaagttt
      241 gtacaaaaaa gttggcgcgc ccaccatgag ttcaggctta tggagccaag aaaaagtcac
      301 ttcaccctac tgggaagagc ggatttttta cttgcttctt caagaatgca gcgttacaga
      361 caaacaaaca caaaagctcc ttaaagtacc gaagggaagt ataggacagt atattcaaga
      421 tcgttctgtg gggcattcaa ggattccttc tgcaaaaggc aagaaaaatc agattggatt
      481 aaaaattcta gagcaacctc atgcagttct ctttgttgat gaaaaggatg ttgtagagat
      541 aaatgaaaag ttcacagagt tacttttggc aattaccaat tgtgaggaga ggttcagcct
      601 gtttaaaaac agaaacagac taagtaaagg cctccaaata gacgtgggct gtcctgtgaa
      661 agtacagctg agatctgggg aagaaaaatt tcctggagtt gtacgcttca gagganccct
      721 gttagcagag aggacagtct ccggaatatt ctttggagtt gaattgctgg aaga
//
