LOCUS       sequence-283452-        1832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1239
FEATURES             Location/Qualifiers
     source          1..1832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacgactt gaaatatgga gtgtatcctc tcaaggaagc aagtggatgc
       61 cctggggctg agaggaatct tctagtttac tcttattttg aaaaggagac attgacattt
      121 agggatgtgg ccatagaatt ctctctggag gagtgggaat gcctgaaccc tgctcagcag
      181 aatttatata tgaatgtgat gttagaaaac tacaaaaacc tggtcttctt gggtgttgct
      241 gtctctaagc aagacccagt cacctgtctg gagcaagaaa aagagccctg gaatatgaag
      301 agacatgaga tggtggatga acccccagct atgtgttctt attttaccaa agacctttgg
      361 ccagagcaag acataaaaga ttcttttcaa caagtaatac tgagaagata tggcaaatgt
      421 gaacatgaga atttacagtt aagaaaaggc tccgcaagtg tagatgagta taaggtgcac
      481 aaagaaggtt ataatgagct aaaccagtgt ttgacaacta cccagagcaa aatatttcca
      541 tgtgataaat atgtgaaagt ctttcataaa tttttaaatg caaatagaca taagacaaga
      601 catactggaa agaaaccttt caaatgtaaa aaatgtggca aatcattttg catgctttta
      661 cacctaagtc aacataaaag aattcatatt agagagaatt cttaccaatg tgaagaatgt
      721 ggcaaagctt ttaaatggtt ctcaaccctt actagacaca agagaattca tactggagag
      781 aaacccttca aatgtgaaga atgtggcaaa gcttttaagc agtcctcaac ccttactaca
      841 cataagataa ttcatactgg ggagaaacca tatagatgtg aagaatgtgg caaagccttc
      901 aaccggtcct cacaccttac tacacataag ataattcata ctggagagaa gccctacaaa
      961 tgtgaagaat gtggcaaagc ttttaaccag tcttcaaccc ttagtacaca taagttcatt
     1021 catgctggag agaaacccta caaatgtgag gaatgtgaca aagcttttaa tcgattctca
     1081 taccttacta aacataagat aattcatact ggagaaaaat cttacaaatg tgaagaatgt
     1141 ggcaaaggct ttaattggtc ctcaaccctt actaaacata aaagaattca tactggagag
     1201 aaaccctaca aatgtgaagt gtgtggcaaa gcctttaatg agtcctcaaa ccttactaca
     1261 cataagatga ttcatactgg agagaaaccc tacaaatgtg aagaatgtgg caaagctttt
     1321 aaccggtccc cacaacttac tgcacataag ataattcata ctggagagaa accttacaaa
     1381 tgtgaagaat gtggcaaagc ttttagccag tcatcaatcc ttactacaca taagagaatt
     1441 cacactggag agaaacccta caaatgtgaa gaatgtggca aagcttttaa ccgatcctca
     1501 aatcttacta aacataagat aattcataca ggagagaaat cttacaaatg tgaagaatgt
     1561 ggtaaagcct ttaaccaatc ctcaactctt actaaacata ggaaaattca tactagacag
     1621 aaaccctaca actgtgaaga atgtgacaat acatttaacc agtcctcaaa ccttattaaa
     1681 caaaataatt catactggag agaaactcta caaatgtcaa gaatgtggga aagcctttag
     1741 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1801 aaggacgacg aatgcagacc ttgcgaaaga ca
//
