LOCUS       sequence-283485-        1322 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1311
FEATURES             Location/Qualifiers
     source          1..1322
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggcaaagt gtggaaacag cagatgtacc ctcagtacgc cacctactat
       61 tacccccagt atctgcaagc caagcagtct ctggtcccag cccaccccat ggcccctccc
      121 agtcccagca ccaccagcag taataacaac agtagcagca gtagcaactc aggatgggat
      181 cagctcagca aaacgaacct ctatatccga ggactgcctc cccacaccac cgaccaggac
      241 ctggtgaagc tctgtcaacc atatgggaaa atagtctcca caaaggcaat tttggataag
      301 acaacgaaca aatgcaaagg ttatggtttt gtcgactttg acagccctgc agcagctcaa
      361 aaagctgtgt ctgccctgaa ggccagtggg gttcaagctc aaatggcaaa gcaacaggaa
      421 caagatccta ccaacctcta catttctaat ttgccactct ccatggatga gcaagaacta
      481 gaaaatatgc tcaaaccatt tggacaagtt atttctacaa ggatactacg tgattccagt
      541 ggtacaagtc gtggtgttgg ctttgctagg atggaatcaa cagaaaaatg tgaagctgtt
      601 attggtcatt ttaatggaaa atttattaag acaccaccag gagtttctgc ccccacagaa
      661 cctttattgt gtaagtttgc tgatggagga cagaaaaaga gacagaaccc aaacaaatac
      721 atccctaatg gaagaccatg gcatagagaa ggagaggtga gacttgctgg aatgacactt
      781 acttacgacc caactacagc tgctatacag aacggatttt atccttcacc atacagtatt
      841 gctacaaacc gaatgatcac tcaaacttct attacaccct atattgcatc tcctgtatct
      901 gcctaccagg tgcaaagtcc ttcgtggatg caacctcaac catatattct acagcaccct
      961 ggtgccgtgt taactccctc aatggagcac accatgtcac tacagcccgc atcaatgatc
     1021 agccctctgg cccagcagat gagtcatctg tcactaggca gcaccggaac atacatgcct
     1081 gcaacgtcag ctatgcaagg agcctacttg ccacagtatg cacatatgca gacgacagcg
     1141 gttcctgttg aggaggcaag tggtcaacag caggtggctg tcgagacgtc taatgaccat
     1201 tctccatata cctttcaacc taataagtag taatgaacta gtccgtaact tgaaagtatt
     1261 tcgatttctt ggctttatat atcttgtgga aaggacgact ccacgctgtt gcgatgttgg
     1321 ac
//
