LOCUS       sequence-283491-        4886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1317
FEATURES             Location/Qualifiers
     source          1..4886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2691..2702
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2934..2945
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accgccacca tgaagttata tgtatttctg gttaacactg gaactactct aacatttgac
       61 actgaactta cagtgcaaac tgtggcagac cttaagcatg ccattcaaag caaatacaag
      121 attgctattc aacaccaggt gctggtggtc aatggaggag aatgcatggc tgcagatcga
      181 agagtgtgta cctacagtgc tgggacggat acaaatccaa tttttctttt taacaaagaa
      241 atgatcttat gtgatcgtcc acctgctatt cctaaaacta ccttttcgac agaaaatgac
      301 atggaaataa aagttgaaga atctcttatg atgcctgcag tttttcatac tgttgcttca
      361 aggacacagc ttgcattgga aatgtatgaa gttgccaaga aactttgttc tttttgtgaa
      421 ggtcttgtac atgatgaaca tcttcaacac caaggctggg ctgcaatcat ggccaacctg
      481 gaggactgtt caaattcata ccaaaagcta cttttcaagt ttgaaagtat ttattcaaat
      541 tatctgcagt ccatagaaga catcaagtta aaacttactc atttaggaac tgcagtttca
      601 gtaatggcca agattccact gttggagtgc ctaaccagac atagttacag agaatgtttg
      661 ggaagactgg attctttacc tgaacatgaa gactcagaaa aagctgagat gaaaagatcc
      721 actgaactgg tgctctctcc tgatatgcct agaacaacta acgaatcttt gttaacctca
      781 tttcccaagt cagtggaaca tgtgtcccca gataccgcag atgctgaaag tggcaaagaa
      841 attagggaat cttgtcaaag tactgttcat cagcaagatg aaactacgat tgacactaaa
      901 gatggtgatc tgcccttttt taatgtctct ttgttagact ggataaatgt tcaagataga
      961 cctaatgatg tggaatcttt ggtcaggaag tgctttgatt ctatgagcag gcttgatcca
     1021 aggattattc gaccatttat agcagaatgc cgtcaaacta ttgccaaact tgataatcag
     1081 aatatgaaag ccattaaagg acttgaagat cggctctacg ccctggacca gatgattgct
     1141 agctgtggcc gactggtgaa tgaacagaaa gagcttgctc agggattttt agctaatcag
     1201 aagagagctg aaaacttaaa ggatgcatct gtattacctg atttatgcct gagtcacgca
     1261 aatcagttga tgattatgtt gcaaaatcat agaaaactgt tagatattaa gcagaagtgt
     1321 accactgcca aacaagaact agcaaataac ctacatgtca gactgaagtg gtgttgcttt
     1381 gtaatgcttc atgctgatca agatggagag aagttacaag ctttgctccg cctcgtaata
     1441 gagctgttag aaagagtcaa aattgttgaa gctcttagta cagttcctca gatgtactgc
     1501 ttagctgttg ttgaggttgt aagaagaaaa atgttcataa aacactacag ggagtgggct
     1561 ggtgctttag tcaaagatgg aaagagatta tatgaagcag aaaaatcaaa aagggaatcc
     1621 tttgggaaat tatttaggaa gtctttttta agaaatcgtc tgtttagggg actggactcc
     1681 tggccccctt ccttttgtac tcaaaagcct cgaaagtttg actgtgaact tccagatatt
     1741 tcattaaaag atttacagtt tctgcaatca ttttgtcctt cggaagttca gccattcctc
     1801 agggttccct tactttgtga ctttgaacct ctacaccagc atgtacttgc tctacataat
     1861 ttggtaaaag cagcacaaag tttggatgaa atgtcacaga ccattacaga tctactgagt
     1921 gaacaaaagg catctgtgag tcagacatcc ccacagtctg cttcttcacc aaggatggaa
     1981 agtacagcag gaattacaac tactacctca ccgagaactc ctccaccact gactgttcag
     2041 gatcccttat gtcctgcagt ttgtccctta gaagaattat ctccagatag tattgatgca
     2101 catacgtttg attttgaaac tattccccat ccaaacatag aacagactat tcaccaagtt
     2161 tctttagact tggattcatt agcagaaagt cctgaatcag attttatgtc tgctgtgaat
     2221 gagtttgtaa tagaagaaaa tttgtcgtct cctaatccta taagtgatcc acaaagccca
     2281 gaaatgatgg tggaatcact ttattcatca gttatcaatg cgatagacag tagacgaatg
     2341 caggatacaa atgtatgtgg taaggaggat tttggagatc atacttctct gaatgtccag
     2401 ttggaaagat gtagagttgt tgcccaagac tctcacttca gtatacaaac cattaaggaa
     2461 gacctttgcc actttagaac atttgtacaa aaagaacagt gtgacttctc aaattcatta
     2521 aaatgtacag cagtagaaat aagaaacatt attgaaaaag taaaatgttc tctggaaata
     2581 acactaaaag aaaaacatca aaaagaacta ctgtctttaa aaaatgaata tgaaggtaaa
     2641 cttgacggac taataaagga aactgaagag aatgaaaaca aaattaaaaa attgaaggga
     2701 gagttagtat gccttgagga ggttttacaa aataaagata atgaatttgc tttggttaaa
     2761 catgaaaaag aagctgtaat ctgcctgcag aatgaaaagg atcagaagtt gttagagatg
     2821 gaaaatataa tgcactctca aaattgtgaa attaaagaac tgaagcagtc acgagaaata
     2881 gtgttagaag acttaaaaaa gctccatgtt gaaaatgatg agaagttaca gttattgagg
     2941 gcagaacttc agtccttgga gcaaagtcat ctaaaggaat tagaggacac acttcaggtt
     3001 aggcacatac aagagtttga gaaggttatg acagaccaca gagtttcttt ggaggaatta
     3061 aaaaaggaaa accaacaaat aattaatcaa atacaagaat ctcatgctga aattatccag
     3121 gaaaaagaaa aacagttaca ggaattaaaa ctcaaggttt ctgatttgtc agacacgaga
     3181 tgcaagttag aggttgaact tgcgttgaag gaagcagaaa ctgatgaaat aaaaattttg
     3241 ctggaagaaa gcagagccca gcagaaggag accttgaaat ctcttcttga acaagagaca
     3301 gaaaatttga gaacagaaat tagtaaactc aaccaaaaga ttcaggataa taatgaaaat
     3361 tatcaggtgg gcttagcaga gctaagaact ttaatgacaa ttgaaaaaga tcagtgtatt
     3421 tccgagttaa ttagtagaca tgaagaagaa tctaatatac ttaaagctga attaaacaaa
     3481 gtaacatctt tgcataacca agcatttgaa atagaaaaaa acctaaaaga acaaataatt
     3541 gaactgcaga gtaaattgga ttcagaattg agtgctcttg aaagacaaaa agatgaaaaa
     3601 attacccaac aagaagagaa atacgaagct attatccaga accttgagaa agacagacaa
     3661 aaattggtca gcagccagga gcaagacaga gaacagttaa ttcagaagct taattgtgaa
     3721 aaagatgaag ctattcagac tgccctaaaa gaatttaaat tggagagaga agttgttgag
     3781 aaagagttat tagaaaaagt taaacatctt gagaatcaaa tagcaaaaag tcctgccatt
     3841 gactctacca gaggagattc ttcaagctta gttgctgaac ttcaagaaaa gcttcaggaa
     3901 gaaaaagcta agtttctaga acaacttgaa gagcaagaaa aaagaaagaa tgaagaaatg
     3961 caaaatgttc gaacatcttt gattgcggaa caacagacca attttaacac tgttttaaca
     4021 agagagaaaa tgagaaaaga aaacataata aatgatctta gtgataagtt gaaaagtaca
     4081 atgcagcaac aagaacggga taaagatttg atagagtcac tttctgaaga tcgagctcgt
     4141 ttgcttgagg aaaagaaaaa gcttgaagaa gaagtcagta agttgcgtag tagcagtttt
     4201 gttccttcac catatgtagc tacagcccca gaactttatg gagcttgtgc acctgaactc
     4261 ccaggtgaat cagatagatc cgctgtggaa acagcagatg aaggaagagt ggattcagca
     4321 atggagacaa gcatgatgtc tgtacaagaa aatattcata tgttgtctga agaaaaacag
     4381 cggataatgc tgttagaacg aacattgcaa ttgaaagaag aagaaaataa acggttaaat
     4441 caaagactga tgtctcagag catgtcttca gtatcttcaa ggcattctga aaagatagct
     4501 attagagatt ttcaggtggg agatttggta ctcatcatcc tagacgaacg ccatgacaat
     4561 tatgtgttat ttactgttag tcctacttta tattttctac attcagagtc tctacctgcc
     4621 ctggatctca aaccaggtga gggtgcttca ggtgcatcta gaagaccctg ggtacttgga
     4681 aaagtaatgg aaaaagaata ctgtcaagcc aaaaaggcac aaaacagatt taaagttcct
     4741 ttggggacaa agttttacag agtgaaagcc gtatcatgga ataagaaagt atagtaatga
     4801 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     4861 gaaaagaagc ggagggatgg taagtt
//
